A spatially resolved nucleic acid biochip based on a gradient of density of immobilized probe oligonucleotide

Park, Sung Han; Krull, Ulrich J.

doi:10.1016/j.aca.2006.01.107

Cited by 18 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various protocols describing the immobilization of oligonucleotides onto surfaces can be found in the literature 23–24, 32–33. Unfortunately, lack of experimental details or characterization aspects prevented us from preparing suitable surfaces for our purposes based on these studies.…”

Section: Resultssupporting

confidence: 90%

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Cottenye

Teixeira

Ponche

et al. 2008

Macromolecular Bioscience

View full text Add to dashboard Cite

Oligonucleotide model surfaces allowing independent variation of topography and chemical composition were designed to study the adhesion and biofilm growth of E.coli. Surfaces were produced by covalent binding of oligonucleotides and immobilization of nucleotide-based vesicles. Their properties were confirmed through a combination of fluorescence microscopy, XPS, ellipsometry, AFM and wettability studies at each step of the process. These surfaces were then used to study the response of three different strains of E.coli quantified in a static biofilm growth mode. This study led to convincing evidence that oligonucleotide-modified surfaces, independent of the topographical feature used in this study, enhanced curli expression without an increase in the number of adherent bacteria.

show abstract

Section: Resultssupporting

confidence: 90%

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Cottenye

Teixeira

Ponche

et al. 2008

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Although the conventional microarray technology employs spatial registration to achieve unprecedented parallelism, it suffers from disadvantages such as additional processing required to print spots on a surface and the associated spatial variation in terms of the quality of the probe immobilization across discrete spots [ 9 , 29 ]. In addition, microarray manufacturing typically involves time-consuming processes and a laboratory environment.…”

Section: Challenges and Recent Progress In Fluorescence Detectionmentioning

confidence: 99%

CMOS Time-Resolved, Contact, and Multispectral Fluorescence Imaging for DNA Molecular Diagnostics

Guo

Cheung

Wong

et al. 2014

Sensors

View full text Add to dashboard Cite

Instrumental limitations such as bulkiness and high cost prevent the fluorescence technique from becoming ubiquitous for point-of-care deoxyribonucleic acid (DNA) detection and other in-field molecular diagnostics applications. The complimentary metal-oxide-semiconductor (CMOS) technology, as benefited from process scaling, provides several advanced capabilities such as high integration density, high-resolution signal processing, and low power consumption, enabling sensitive, integrated, and low-cost fluorescence analytical platforms. In this paper, CMOS time-resolved, contact, and multispectral imaging are reviewed. Recently reported CMOS fluorescence analysis microsystem prototypes are surveyed to highlight the present state of the art.

show abstract

“…The absorptivity or the fraction of the excitation light absorbed by the fluorophores can be expressed as (3) The excitation photon rate, , is the rate at which excitation photons are successfully absorbed by the fluorophore sample, given by (4) where is the power of the excitation light that falls onto the fluorophore sample area , given by (5) where and are the power and the total illuminated area of the excitation source, respectively.…”

Section: A Microsystem Modelmentioning

confidence: 99%

“…Although the conventional microarray technology employs spatial registration to achieve unprecedented parallelism, it suffers from disadvantages such as additional processing required to print spots on a surface and the associated spatial variation in terms of the quality of the probe immobilization across discrete spots [2], [3]. Also, the hybridization chemistry requires the washing away of samples after introducing them to the probes.…”

Section: Introductionmentioning

confidence: 99%

CMOS Spectrally-Multiplexed FRET-on-a-Chip for DNA Analysis

Noor

Krull

et al. 2013

IEEE Trans. Biomed. Circuits Syst.

View full text Add to dashboard Cite

A spectral-multiplexed fluorescence contact imaging microsystem for DNA analysis is presented. The microsystem integrates a filterless CMOS Color PhotoGate (CPG) sensor that exploits the polysilicon gate as an optical filter, and therefore does not require an external color filter. The CPG is applied to fluorescence-based transduction in a spectrally multiplexed format by differentiating among multiple emission bands, hence replacing the functionality of a bank of emission filters. A microsystem has been quantitatively modeled and prototyped based on the CPG fabricated in a standard 0.35 μm CMOS technology. The multi-color imaging capability of the microsystem in analyzing DNA targets has been validated in the detection of marker gene sequences for spinal muscular atropy disease and Escherichia coli (E. coli). Spectral-multiplexing enables the two DNA targets to be simultaneously detected with a measured detection limits of 240 nM and 210 nM for the two target concentrations at a sample volume of 10 μL for the green and red transduction channels, respectively.

show abstract

A spatially resolved nucleic acid biochip based on a gradient of density of immobilized probe oligonucleotide

Cited by 18 publications

References 21 publications

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

CMOS Time-Resolved, Contact, and Multispectral Fluorescence Imaging for DNA Molecular Diagnostics

CMOS Spectrally-Multiplexed FRET-on-a-Chip for DNA Analysis

Contact Info

Product

Resources

About