A spatial–temporal understanding of gene regulatory networks and NtARF-mediated regulation of potassium accumulation in tobacco

Wang, Xueqing; Wang, Bing‐Wu; Song, Zhiwei; Zhao, Lu; Ruan, Wenyuan; Gao, Yulong; Jia, Xianqing; Yi, Keke

doi:10.1007/s00425-021-03790-2

Cited by 6 publications

(6 citation statements)

References 50 publications

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“…An increase in potassium content can significantly improve the elasticity and combustibility of tobacco, reduce the content of harmful substances such as tar and CO in smoke, and improve the quality of tobacco. 4 , 5 …”

Section: Introductionmentioning

confidence: 99%

The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco

Xu,

Lu,

Jiang

et al. 2024

Plant Signaling & Behavior

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Section: Introductionmentioning

confidence: 99%

The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco

Xu,

Lu,

Jiang

et al. 2024

Plant Signaling & Behavior

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“…Potassium ion (K + ) is a macroelement required for plant growth and affects the yield, quality, and stress resistance of plants by participating in activities such as protein synthesis, cell osmotic regulation, photosynthesis, stomatal movement, and enzyme activation (Pettigrew, 2008;Ragel et al, 2019). Tobacco is a potassium-loving crop, and potassium can not only significantly improve the color, combustion power, and firepower of tobacco leaves but also increase their flexibility and softness (Wang et al, 2022). Therefore, high potassium content in tobacco leaves increases the yield and quality of the leaves.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis between inbred lines and hybrids provides molecular insights into K+ content heterosis of tobacco (Nicotiana tabacum L.)

Luo

et al. 2022

Front. Plant Sci.

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Potassium (K+) is essential for crop growth. Increasing the K+ content can often directly promote the improvement of crop yield and quality. Heterosis plays an important role in genetic improvement and leads to genetic gains. We found that the K+ content of tobacco showed significant heterosis, which is highly significant for cultivating tobacco varieties with high K+ content. However, the mechanism by which K+ content heterosis occurs in tobacco leaves is not clear. In this study, a comprehensive comparative transcriptome sequencing analysis of root samples from the hybrid G70 × GDH11 and its parental inbred lines G70 and GDH11 was performed to elucidate the importance of the root uptake capacity of K+ in the formation of heterosis. The results showed that 29.53% and 60.49% of the differentially expressed genes (DEGs) exhibited dominant and over-dominant expression patterns, respectively. These non-additive upregulated DEGs were significantly enriched in GO terms, such as metal ion transport and reaction, ion balance and homeostasis, ion channel activity, root meristem growth, and regulation of root hairs. The KEGG annotation results indicated that these genes were mainly involved in the pathways such as energy metabolism, carbohydrate formation, amino acid metabolism, and signal transduction. Further analysis showed that probable potassium transporter 17 (NtKT17) and potassium transporter 5-like (NtKT5), associated with potassium ion absorption, glutamate receptor 2.2-like and glutamate receptor 2.8-like, associated with ion channel activity, LOC107782957, protein detoxification 42-like, and probable glutamate carboxypeptidase 2, associated with root configuration, showed a significantly higher expression in the hybrids. These results indicated that the over-dominant expression pattern of DEGs played a key role in the heterosis of K+ content in tobacco leaves, and the overexpression of the genes related to K+ uptake, transport, and root development in hybrids helped to improve the K+ content of plants, thus showing the phenomenon of heterosis.

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“…Wang and Wu revealed that K + influences plant yield, quality, and stress resistance [ 7 ] through its involvement in protein synthesis, cellular osmoregulation, photosynthesis, stomatal movement, and enzyme activation [ 8 ]. Moreover, we were informed that K + was crucial for tobacco leaf quality, which could significantly improve tobacco leaves’ color, combustion force, and fire maintenance power, moderate tobacco leaves’ identity, and increase their elasticity and softness [ 9 ]. The potassium content of tobacco leaves changes abruptly during the topping period and decreases rapidly after topping, resulting in a low potassium content in mature tobacco leaves, which is the focus of research on tobacco quality [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, we were informed that K + was crucial for tobacco leaf quality, which could significantly improve tobacco leaves’ color, combustion force, and fire maintenance power, moderate tobacco leaves’ identity, and increase their elasticity and softness [ 9 ]. The potassium content of tobacco leaves changes abruptly during the topping period and decreases rapidly after topping, resulting in a low potassium content in mature tobacco leaves, which is the focus of research on tobacco quality [ 9 ]. Potassium fertilizer application is a common technology to improve crop yield and quality, but the high input of K + fertilizer and low utilization rate increase the production cost and cause environmental problems [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Overdominant expression of related genes of ion homeostasis improves K+ content advantage in hybrid tobacco leaves

Luo

et al. 2022

BMC Plant Biol

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Background Potassium(K+) plays a vital role in improving the quality of tobacco leaves. However, how to improve the potassium content of tobacco leaves has always been a difficult problem in tobacco planting. K+ content in tobacco hybrid is characterized by heterosis, which can improve the quality of tobacco leaves, but its underlying molecular genetic mechanisms remain unclear. Results Through a two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 with weak heterosis were screened out. Transcriptome analyses revealed that 80.89% and 57.28% of the differentially expressed genes (DEGs) in the strong and weak heterosis combinations exhibited an overdominant expression pattern, respectively. The genes that up-regulated the overdominant expression in the strong heterosis hybrids were significantly enriched in the ion homeostasis. Genes involved in K+ transport (KAT1/2, GORK, AKT2, and KEA3), activity regulation complex (CBL-CIPK5/6), and vacuole (TPKs) genes were overdominant expressed in strong heterosis hybrids, which contributed to K+ homeostasis and heterosis in tobacco leaves. Conclusions K+ homeostasis and accumulation in tobacco hybrids were collectively improved. The overdominant expression of K+ transport and homeostasis-related genes conducted a crucial role in the heterosis of K+ content in tobacco leaves.

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A spatial–temporal understanding of gene regulatory networks and NtARF-mediated regulation of potassium accumulation in tobacco

Cited by 6 publications

References 50 publications

The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco

The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco

Comparative transcriptome analysis between inbred lines and hybrids provides molecular insights into K+ content heterosis of tobacco (Nicotiana tabacum L.)

Overdominant expression of related genes of ion homeostasis improves K+ content advantage in hybrid tobacco leaves

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