A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy

Michalet, Xavier; Siegmund, Oswald H. W.; Vallerga, J. V.; Jelinsky, Patrick; Millaud, J.; Weiss, Shimon

doi:10.1117/12.646482

Cited by 18 publications

(22 citation statements)

References 17 publications

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“…The H33D detector is based on a previously described custom-designed S20 photocathode+MCP stack+XDL anode assembly sealed in a vacuum tube 8,10,11 . Its principle is schematized in Fig.…”

Section: High-throughput High Temporal and High Spatial Wide-field Smentioning

confidence: 99%

Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector

Michalet

Siegmund

Vallerga

et al. 2006

Advanced Photon Counting Techniques

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We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and high-spatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.

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“…The H33D detector is based on a previously described custom-designed S20 photocathode+MCP stack+XDL anode assembly sealed in a vacuum tube 8,10,11 . Its principle is schematized in Fig.…”

Section: High-throughput High Temporal and High Spatial Wide-field Smentioning

confidence: 99%

Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector

Michalet

Siegmund

Vallerga

et al. 2006

Advanced Photon Counting Techniques

Self Cite

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“…Microchannel plate (MCP) imaging detector schemes [1][2][3][4][5][6][7][8] have been used in many NASA missions (e.g. FUSE, GALEX and COS on Hubble) as well as other non-astronomical applications.…”

Section: Introductionmentioning

confidence: 99%

Large-format high-spatial resolution cross-strip readout MCP detectors for UV astronomy

Vallerga

Raffanti²,

Tremsin

et al. 2010

Space Telescopes and Instrumentation 2010: Ultraviolet to Gamma Ray

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We have implemented cross strip readout microchannel plate detectors in a 40mm diameter active area format, open face (UV/particle) configuration. These have been tested with a field programmable gate array based parallel channel electronics for event encoding which can process high input event rates (> 5 MHz) with high spatial resolution. Using small pore MCPs (6 m) operated in a pair, we achieved spatial resolution of < 20 m FHWM at MCP gain of 1x10 6 eper event. Future large aperture UV missions require detectors to have large formats (> 100 mm) with high event rate throughput (~ MHz) while retaining high spatial resolution. We will discuss our plans to scale our 47 mm square anodes to 100 mm and our ideas for the next front end ASIC that combines a state-of-the-art, fast charge sensitive amplifier with fast sampling analog storage and built in ADCs.

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“…Microchannel plate (MCP) imaging detector schemes [1][2][3][4][5][6][7][8] have found extensive uses in many fields. We are developing a new generation of MCP detector using the Cross Strip (XS) anode.…”

Section: Introductionmentioning

confidence: 99%

Cross strip microchannel plate imaging photon counters with high time resolution

et al. 2010

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We have implemented cross strip readout microchannel plate detectors in 18 mm active area format including open face (UV/particle) and sealed tube (optical) configurations. These have been tested with a field programmable gate array based parallel channel electronics for event encoding which can process high input event rates (> 5 MHz) with high spatial resolution. Using small pore MCPs (6 µm) operated in a pair, we achieve gains of >5 x 10 5 which is sufficient to provide spatial resolution of <35 µm FHWM, with self triggered event timing accuracy of ~2 ns for sealed tube optical sensors. A peak quantum efficiency of ~19% at 500 nm has been achieved with SuperGenII photocathodes that have response over the 400 nm to 900 nm range. Local area counting rates of up to >200 events/mcp pore sec -1 have been attained, along with image linearity and stability to better than 50 µm.

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A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy

Cited by 18 publications

References 17 publications

Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector

Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector

Large-format high-spatial resolution cross-strip readout MCP detectors for UV astronomy

Cross strip microchannel plate imaging photon counters with high time resolution

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