A soluble recombinant fusion protein of the transmembrane envelope protein of equine infectious anaemia virus for ELISA

Antibody response to Epstein-Barr virus Rta protein in patients with nasopharyngeal carcinoma

“…Furthermore, another fragment of Rta, R185I, that could bind to the antibodies, as described previously, 13 also was included. The E. coli-produced, recombinant fusion proteins retained the Rta antigenicity, and, similar to previous reports, 21,22 the GST fragment in the fusion protein did not reveal any reactivity to the sera in our results and, thus, was not removed from the recombinant protein fragments.…”

Equine arteritis virus-neutralizing antibody in the horse is induced by a determinant on the large envelope glycoprotein GL

“…The pGEX bacterial expression system allows the production of abundant, easily purified fusion proteins (Smith & Johnson, 1988) which are useful for defining virus epitopes (Lopez de Turiso et al, 1991) and as ELISA and vaccine antigens (Johnson et al, 1989;Thomas et al, 1992). In this study we have utilized GST fusion proteins to identify the EAV G L protein as an important viral antigen; post-infection, VN equine sera predominantly recognized bacterial fusion proteins containing GL.…”

Detection of Antibodies to Ovine Lentivirus using Recombinant Capsid and Transmembrane Proteins

“…Higher sensitivity of the rTM antigen could be explained by the aforementioned¯uctuation in production of antibodies against different viral proteins. Furthermore, transmembrane glycoprotein was found to be an immunodominant component of human and animal lentiviruses (Mcguire et al, 1992 7 ; Thomas et al, 1992;8 Bertoni et al, 1994;Eberle et al, 1997). In conclusion, recombinant histidine-tagged capsid and transmembrane proteins showed suf®cient sensitivity and speci®city in detecting antibodies against OvLV compared to whole virus immunoblot.…”