A soluble inhibitor of B and T cell proliferation and antibody synthesis produced by dividing human T cells

Wolf, Raoul L.; Whitsed, Helen; Rosen, Fred S.; Merler, Ezio

doi:10.1016/0008-8749(78)90267-8

Cited by 17 publications

(13 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity was destroyed by heating to 60" C for 15 min, as we have noted for SIF (14). During ion-exchange chromatography of placental cell culture fluids, SIF-like activity eluted only in the range of 40-50 mM POP buffer; i.e.…”

Section: Resultsmentioning

confidence: 71%

“…There was no increase in suppressor activity beyond the level generated in the first 24-h period. Release of SIF by placental giant cells without prior activation is distinct from MLR, where 5-6 days of culture are required to generate SIF (14). This time interval correlates with activation of lymphocytes by MLR Shou et al (20) have demonstrated that unstimulated lymphocytes in culture produce a suppressor substance that inhibits MLR in the proliferative phase, indicating that lymphocytes can release suppressor substances without stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…MNC settling at the interface were washed three times in HBSS and resuspended in complete medium at lo6 cells/ml. SIF-containing culture fluid was prepared by MLR as described elsewhere (14). Briefly, MNC were derived from unrelated donors and each resuspended in complete medium at 5 X lo6 cells/ml.…”

Section: Placental Cell Culturesmentioning

confidence: 99%

“…Uptake of ['HITdR into new DNA was measured by counts remaining on the filter discs using a fluor-based cocktail (RPI-3a, Research Products International, Mount Prospect, IL). LSS activity was measured with a shorter culture period of 48 h, as we have previously noted peak suppression by LSS at this time (14).…”

Section: Placental Cell Culturesmentioning

confidence: 99%

“…SIF nonspecifically suppresses lymphocyte proliferation and antibody synthesis (14). It is released by dividing T and null cells, but not by B cells or continually growing B or T cell lines.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Human Placental Cells that Regulate Lymphocyte Function

Wolf

1988

Pediatr Res

View full text Add to dashboard Cite

A B S T R A a . We examined normal human placenta for an immunologic function by measuring the release of soluble inhibitory factor (SIF). SIF is a product of normal T lymphocytes and of the JEG-3 choriocarcinoma cell line, and blocks proliferative and antibody-producing responses of mononuclear cells. SIF can be further characterized by a noncovalently linked subcomponent, lipid suppressor substance. The villous surfaces of six normal human placentae were digested with collagenase to obtain a population of predominantly multinucleated giant cells. These cells were maintained in standard culture for 5 days after which the cell-free conditioned culture medium was assayed for SIF content by measuring suppression of VH] thymidine incorporation into lymphocytes stimulated by low-dose phytohemagglutinin. Undiluted placental SIF induced 88% inhibition of this response (p < 0.001). The placental SIF was found to contain lipid suppressor substance, as does SIF from mononuclear cells. We determined this by thin-layer chromatography where a peak of suppressive activity occurred at Rf 0.32 (PHjthymidine incorporation reduced from 21810 + 308 to 4121 2 214 cpm); this is the position on thin-layer chromatography to which mononuclear cell lipid suppressor substance migrates. Ion exchange chromatography comparing the elution patterns of lymphocyte-SIF and placental-SIF indi: cated that both eluted in the fraction of 40-50 mM P W buffer, further suggesting identity between these two substances. SIF from placental and lymphocyte sources functioned by inducing the presence of suppressor cells in culture. Mononuclear cells were incubated for 48 h in SIF; the resultant cell population reduced [H3]thymidine incorporation into phytohemagglutinin-stimulated lymphocytes from 21170 + 1721 to 8612 2 311 cpm (60% inhibition, p < 0.001). Adherent cells were not required for function of these cells and they were not sensitive to mitomycin C. The placenta helps prevent immunologic rejection of the fetus by several mechanisms; it functions as a barrier, it is devoid of human histocompatibility leukocyte antigen markers and it has immunologic functions. The studies presented herein indicate that the placenta releases a substance that induces the formation of suppressor cells. These data support the hypothesis that placental cells have an immunoregulatory function. (Pediatr Res 23: 212-218, 1988) Abbreviations Ec, effector cells FCS, fetal calf serum PH]TdR, tritiated thymidine

show abstract

Section: Resultsmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Placental Cell Culturesmentioning

confidence: 99%

Section: Placental Cell Culturesmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Human Placental Cells that Regulate Lymphocyte Function

Wolf

1988

Pediatr Res

View full text Add to dashboard Cite

show abstract

Suppression of mitogen‐induced peripheral B cell differentiation by soluble Fcγ receptors released from lymphocytes

et al. 1980

View full text Add to dashboard Cite

Soluble receptors for FcIgG released from unstimulated human peripheral blood lymphocytes were isolated by affinity chromatography on Sepharose 4B-IgG. This material was shown to interfere with the differentiation of peripheral blood B cells into Ig-secreting cells in cultures stimulated with pokeweek or Nocardia opaca extracts. Neither cell viability nor [3H]thymidine incorporation were altered, but the number of Ig-containing cells and that of Ig-secreting cells were decreased. These effects were dose-related. They were found to be associated with Fc IgG-binding soluble material, since absorption on Sepharose 4B-IgG but not on pepsin-digested F(ab')2 fragments removed the inhibitory activity. This suppressor factor, released by unstimulated lymphocytes, may represent a human analogue of murine immunoglobulin-binding factor (IBF) produced by alloactivated T cells.

show abstract

Human nonspecific suppressive lymphokines

Halpern

1991

J Clin Immunol

View full text Add to dashboard Cite

Since the term "lymphokine" first appeared in print over 20 years ago, a tremendous number of these soluble mediators of the immune system have been described. Within the past few years, many human nonspecific suppressive lymphokines have been identified. This review discusses the historical basis of immunologic suppression and suppressor factors. Later reports describing suppressive human lymphokines are then grouped into four categories: primarily stimulatory lymphokines that also mediate certain suppressive activities, suppressive lymphokines produced during altered states of immunity, suppressive lymphokines produced by exogenously stimulated lymphocytes, and suppressive lymphokines produced by unstimulated lymphocytes. Recent work I have been involved in focusing on the human suppressive lymphokine soluble suppressor factor (SSF) is also discussed.

show abstract

A soluble inhibitor of B and T cell proliferation and antibody synthesis produced by dividing human T cells

Cited by 17 publications

References 18 publications

Human Placental Cells that Regulate Lymphocyte Function

Human Placental Cells that Regulate Lymphocyte Function

Suppression of mitogen‐induced peripheral B cell differentiation by soluble Fcγ receptors released from lymphocytes

Human nonspecific suppressive lymphokines

Contact Info

Product

Resources

About