A soluble form of the human CD8 alpha chain expressed in the baculovirus system: biochemical characterization and binding to MHC class I

Tyrosine 319 in the Interdomain B of ZAP-70 Is a Binding Site for the Src Homology 2 Domain of Lck

“…31-13 T-cell line, a derivative of the Jurkat T-cell line, which does not express the TCR due to the lack of the TCR ␤ chain transcript (22), was maintained in complete RPMI 1640 medium. ␤WT160 was obtained from 31-13 cells by stable transfection of the TCR ␤-chain (22) and maintained in complete RPMI 1640 medium also containing 2 g/liter of G-418 (Life Technologies, Inc.). Jurkat stable transfectants expressing ZAP-70-WT (clone 15.8), ZAP-70-Y319F (clones 1.40 and 1.60), and ZAP-70Y315F (clones 2.21/14 and 2.21/2) were previously described in Ref.…”

T-cell receptor gene homologs are present in the most primitive jawed vertebrates.

“…The highest number of identities is found in the extracellular domain. A functionally significant lysine residue, which interacts with CD3 in mammals (24), is present at position 269 in HF6, as well as in all TCR genes.…”

Crystal structure of a human CD3-ε/δ dimer in complex with a UCHT1 single-chain antibody fragment

“…TCR͞CD3 chains assemble into a minimal eight-subunit complex in the endoplasmic reticulum through a series of dimeric and trimeric interactions with a stoichiometry of one TCR-␣͞␤ heterodimer, one CD3-͞␥ heterodimer, one CD3-͞␦ heterodimer, and a TCR-homodimer (4)(5)(6)(7)(8). Whereas extracellular contacts are sufficient for interactions within TCR and CD3 heterodimers (9)(10)(11)(12), transmembrane interactions are necessary for assembly and surface expression of intact TCR͞CD3 complexes (7,(13)(14)(15)(16)(17)(18). Extracellular domains of CD3 may provide additional specificity to mandatory transmembrane interactions (8,19,20), but specific extracellular interactions between TCR and CD3-͞␦ or CD3-͞␥ have yet to be determined.…”