A solid-solution theory of anesthetic interaction with lipid membranes: temperature span of the main phase transition

Antimicrobial Nonapeptide Leucinostatin A-Dependent Effects on the Physical Properties of Phospholipid Model Membranes

“…Correlations between the previously described weak ionophore properties of the drug (13) and its interactions with membrane bilayers are supported by comparing the influence induced by Leu A concentration on 45 Ca 2+ uptake by mouse thymocytes and artificial membranes (Fig. 11).…”

“…Eagle's minimum essential medium was from Behring Werke A.G. (Marburg, Germany). 45 CaCl 2 (4.3 Gbq/mmol) was purchased from Amersham International plc (Buckinghamshire, U.K.). Hepes buffer was obtained from Sigma Chemical Co. (St. Louis, Missouri).…”

“…A Smoluchowsky constant, F(K a ), of 1.5 was used to achieve ζ -potential values from electrophoretic mobility. 45 Ca 2+ Uptake 45 Ca 2+ uptake determination was carried out elsewhere as reported (35). Briefly, mouse thymocytes, prepared as previously described (36), were incubated in Eagle's minimum essential medium supplemented with 20 mM Hepes (pH 7.4) at a cell density of 10 7 cells/ml, while DPPC artificial membranes were suspended in 50 mM Hepes buffer (pH 7.4).…”

“…Briefly, mouse thymocytes, prepared as previously described (36), were incubated in Eagle's minimum essential medium supplemented with 20 mM Hepes (pH 7.4) at a cell density of 10 7 cells/ml, while DPPC artificial membranes were suspended in 50 mM Hepes buffer (pH 7.4). Suitable Leu A amounts and 40 kBq/ml 45 CaCl 2 were added to the cells or liposome suspensions and incubated at 37…”

“…The final sample volume was 1 ml and the final 45 Ca 2+ concentration was 1. sions were then centrifuged (25,000× g for 30 min at 5…”