A solid-phase immunofluorescence assay (SIFA) for screening antigen-specific hybridomas

Micheel, Burkhard; Karsten, Uwe; Fiebach, H

doi:10.1016/0022-1759(81)90330-6

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Solid Phase Immunoassay1

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1982

1990

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Cited by 9 publications

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“…Purified subunits A or B (2 ]lg/50 jl) from either E.coZi or B.subtiZis were incubated in polyvinyl chloride wells (16). After 2 h at 370C the wells were washed several times with phosphate-saline buffer containing 1 mg/ml bovine serum albumin (buffer A).…”

Section: Solid Phase Immunoassaymentioning

confidence: 99%

DNA binding and antigenic specifications of DNA gyrase

Lother

Orr

1984

Nucl Acids Res

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Complexes of DNA gyrase and minichromosomal DNA containing the origin of replication of Escherichia coli (oriC) can be formed without metabolic energy and visualised by electron microscopy. The A subunit, part of the A2B2-DNA gyrase complex is the binding protein. Various binding sites are scattered around the minichromosomal DNA including oriC. The minimal origin contains the only prominent and reproducible binding site. Binding to this site is suppressed by oxolinic acid and the ATP analogue beta-y-imido ATP. If gyrase isolated from the gram-positive bacterium Bacillus subtilis is used no binding to oriC is seen. This observation is consistent with antigenic differences between the A subunits of the two microorganisms. The binding to oriC might reflect a requirement for DNA gyrase during the initiation of DNA replication.

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Section: Solid Phase Immunoassaymentioning

confidence: 99%