A snoRNA modulates mRNA 3′ end processing and regulates the expression of a subset of mRNAs

Huang, Chunliu; Shi, Junjie; Guo, Yinglu; Huang, Weijun; Huang, Shanshan; Ming, Siqi; Wu, Xingui; Zhang, Rui; Ding, Junjun; Zhao, Wei; Jia, Jinsheng; Huang, Xi; Xiang, Andy Peng; Shi, Yongsheng; Yao, Chengguo

doi:10.1093/nar/gkx651

Cited by 75 publications

(89 citation statements)

References 61 publications

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“…The role of noncoding RNAs in both 3′ end processing and DDR has not been extensively studied. Recently, a small nucleolar RNA (snoRNA), normally associated with rRNA maturation, was shown to compete with CpA component Fip1 for binding to AAUAAA signal (Huang et al, ; J. Shi, Huang, Huang, & Yao, ). SNORD50A inhibits mRNA 3′ processing by blocking the Fip1‐poly(A) site interaction, and SNORD50A depletion changed APA profiles genome‐wide.…”

Section: Alternative Polyadenylationmentioning

confidence: 99%

Connections between 3′ end processing and DNA damage response: Ten years later

Murphy

Kleiman

2019

WIREs RNA

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Ten years ago we reviewed how the cellular DNA damage response (DDR) is controlled by changes in the functional and structural properties of nuclear proteins, resulting in a timely coordinated control of gene expression that allows DNA repair. Expression of genes that play a role in DDR is regulated not only at transcriptional level during mRNA biosynthesis but also by changing steady‐state levels due to turnover of the transcripts. The 3′ end processing machinery, which is important in the regulation of mRNA stability, is involved in these gene‐specific responses to DNA damage. Here, we review the latest mechanistic connections described between 3′ end processing and DDR, with a special emphasis on alternative polyadenylation, microRNA and RNA binding proteins‐mediated deadenylation, and discuss the implications of deregulation of these steps in DDR and human disease. This article is categorized under: RNA Processing > 3′ End Processing RNA‐Based Catalysis > Miscellaneous RNA‐Catalyzed Reactions RNA in Disease and Development > RNA in Disease

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Section: Alternative Polyadenylationmentioning

confidence: 99%

Connections between 3′ end processing and DNA damage response: Ten years later

Murphy

Kleiman

2019

WIREs RNA

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“…Thus, snoRNAs play a vital role in the genesis and processing of the ribosome. Modification of the ribosome has been implicated in cancer development, indicating that the classical functions of snoRNAs might contribute to cancer occurrence and development, and this is a new field that requires further researches [5-7]. …”

Section: Introductionmentioning

confidence: 99%

C/D-Box Snord105b Promotes Tumorigenesis in Gastric Cancer via ALDOA/C-Myc Pathway

Zhang¹,

Zhao²,

Wu³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Small nucleolar RNAs (snoRNAs) play an important role in carcinogenesis. In this study, we identified a C/D box snoRNA, snord105b, and further investigated the function and mechanism of the snord105b in gastric cancer (GC). Methods: The expression level of snord105b in GC tissures, sera and cell lines were detected by qRT-PCR. Cell viability was assessed using MTS assay. Transwell and wound healing assay were performed to evaluate migration and invasion, and protein expression was examined by western blotting. ChIRP and MS analysis was used to seek for the special binding protein of snord105b. Results: The snord105b was upregulated and associated with tumor size, differentiation, and pathological stage in GC. Snord105b affected proliferation, migration and invasion in multiple GC cell lines. The oncoqenic activity of snord105b was also confirmed with in vivo data. Mechanistically, snord105b specifically bound to ALDOA and affected C-myc, which plays a key role in carcinogenesis and tumor development. Conclusion: Snord105b appears to be a novel oncogene and is clinically and functionally involved in the development of GC. Targeting snord105b and its pathway may provide new biomarkers or potential treatments for patients with GC.

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“…Notably, almost all protocols that had been previously applied for 3 0 processing factors purification do not include the identification of trans-acting RNAs [3,10], if there exist any. To systematically characterize mRNA 3 0 processing complex, we have recently purified RNAs that are associated with this complex [11]. Strikingly, snoRNAs were almost all the high-confidence candidates.…”

Section: Introductionmentioning

confidence: 99%

snoRNAs associate with mRNA 3′ processing complex: New wine in old bottles

Shi

Huang

et al. 2017

RNA Biology

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3' end processing is required for the maturation of all eukaryotic RNAs. Current model suggests that canonical mRNA 3' processing is carried out exclusively within a protein complex termed mRNA 3' processing complex. In a recent study, by using RNA-biotin based pull-down assay and high-throughput sequencing, we reported that a subset of small nucleolar RNAs (snoRNAs) were physically associated with this macromolecular machinery. Through detailed characterization of one of these snoRNAs, SNORD50A, we revealed that non-coding RNA, such as snoRNA, may play a regulatory role in mRNA 3' processing. Our results provided novel insight into both the regulatory mechanism of mRNA 3' processing and the non-canonical functions of snoRNAs.

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A snoRNA modulates mRNA 3′ end processing and regulates the expression of a subset of mRNAs

Cited by 75 publications

References 61 publications

Connections between 3′ end processing and DNA damage response: Ten years later

Connections between 3′ end processing and DNA damage response: Ten years later

C/D-Box Snord105b Promotes Tumorigenesis in Gastric Cancer via ALDOA/C-Myc Pathway

snoRNAs associate with mRNA 3′ processing complex: New wine in old bottles

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