A Snapshot of Direct Cell–Cell Communications in Wound Healing and Scarring

Ehrlich, H. Paul

doi:10.1089/wound.2012.0414

Cited by 28 publications

(22 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cell aggregates on the Bare‐PDMS and PAA‐PDMS emitted strong fluorescence signals, while the fibroblasts confined within the micropatterns showed significantly suppressed α‐SMA expression (Figure ). The aggregation of the fibroblasts was known to promote transformation to the myofibroblasts as described in previous studies . Therefore, the inhibition of cell aggregation could have a positive effect in reducing FBRs.…”

Section: Discussionmentioning

confidence: 68%

“…The aggregation of the fibroblasts was known to promote transformation to the myofibroblasts as described in previous studies. [15,43] Therefore, the inhibition of cell aggregation could have a positive effect in reducing FBRs. The hole and stripe patterns successfully prevent increase in the cell aggregation by confining the fibroblasts inside holes and grooves, respectively.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Modulation of Foreign Body Reaction against PDMS Implant by Grafting Topographically Different Poly(acrylic acid) Micropatterns

Lee

Shin

Yoo

et al. 2019

Macromolecular Bioscience

View full text Add to dashboard Cite

The surface of poly(dimethylsiloxane) (PDMS) is grafted with poly(acrylic acid) (PAA) layers via surface‐initiated photopolymerization to suppress the capsular contracture resulting from a foreign body reaction. Owing to the nature of photo‐induced polymerization, various PAA micropatterns can be fabricated using photolithography. Hole and stripe micropatterns ≈100‐µm wide and 3‐µm thick are grafted onto the PDMS surface without delamination. The incorporation of PAA micropatterns provides not only chemical cues by hydrophilic PAA microdomains but also topographical cues by hole or stripe micropatterns. In vitro studies reveal that a PAA‐grafted PDMS surface has a lower proliferation of both macrophages (Raw 264.7) and fibroblasts (NIH 3T3) regardless of the pattern presence. However, PDMS with PAA micropatterns, especially stripe micropatterns, minimizes the aggregation of fibroblasts and their subsequent differentiation into myofibroblasts. An in vivo study also shows that PDMS samples with stripe micropatterns polarized macrophages into anti‐inflammatory M2 macrophages and most effectively inhibits capsular contracture, which is demonstrated by investigation of inflammation score, transforming‐growth‐factor‐β expression, number of macrophages, and myofibroblasts as well as the collagen density and capsule thickness.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Modulation of Foreign Body Reaction against PDMS Implant by Grafting Topographically Different Poly(acrylic acid) Micropatterns

Lee

Shin

Yoo

et al. 2019

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…During scar development, myofibroblasts accumulate quickly and became the dominant cell phenotype. After scar development, the myofibroblasts either undergo apoptosis or revert into fibroblasts over time [26]. However, healing of severe tissue loss or conditions of inflammation can induce abnormal myofibroblast formation and can result in excessive scarring or even organ or tissue contraction.…”

Section: Discussionmentioning

confidence: 99%

Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomal MicroRNAs Suppress Myofibroblast Differentiation by Inhibiting the Transforming Growth Factor-β/SMAD2 Pathway During Wound Healing

Fang

Zhang

et al. 2016

Stem Cells Translational Medicine

458

331

View full text Add to dashboard Cite

Excessive scar formation caused by myofibroblast aggregations is of great clinical importance during skin wound healing. Studies have shown that mesenchymal stem cells (MSCs) can promote skin regeneration, but whether MSCs contribute to scar formation remains undefined. We found that umbilical cord-derived MSCs (uMSCs) reduced scar formation and myofibroblast accumulation in a skin-defect mouse model. We found that these functions were mainly dependent on uMSC-derived exosomes (uMSC-Exos) and especially exosomal microRNAs. Through high-throughput RNA sequencing and functional analysis, we demonstrated that a group of uMSC-Exos enriched in specific microRNAs (miR-21, -23a, -125b, and -145) played key roles in suppressing myofibroblast formation by inhibiting the transforming growth factor-b2/SMAD2 pathway. Finally, using the strategy we established to block miRNAs inside the exosomes, we showed that these specific exosomal miRNAs were essential for the myofibroblast-suppressing and anti-scarring functions of uMSCs both in vitro and in vivo. Our study revealed a novel role of exosomal miRNAs in uMSC-mediated therapy, suggesting that the clinical application of uMSC-derived exosomes might represent a strategy to prevent scar formation during wound healing. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:1425-1439 SIGNIFICANCEExosomes have been identified as a new type of major paracrine factor released by umbilical cordderived mesenchymal stem cells (uMSCs). They have been reported to be an important mediator of cell-to-cell communication. However, it is still unclear precisely which molecule or group of molecules carried within MSC-derived exosomes can mediate myofibroblast functions, especially in the process of wound repair. The present study explored the functional roles of uMSC-exosomal microRNAs in the process of myofibroblast formation, which can cause excessive scarring. This is an unreported function of uMSC exosomes. Also, for the first time, the uMSC-exosomal microRNAs were examined by high-throughput sequencing, with a group of specific microRNAs (miR-21, miR-23a, miR-125b, and miR-145) found to play key roles in suppressing myofibroblast formation by inhibiting excess a-smooth muscle actin and collagen deposition associated with activity of the transforming growth factor-b/SMAD2 signaling pathway.

show abstract

“…For example a key aspect to promote cell growth is the pore size at different scales. The new research developed to understand cell-cell and cell-ECM interactions [11], have emphasized the need to preserve the properties of the ECM of a normal wound. In this sense, it is also important the use of mixing material better than single materials [10].…”

Section: Skin Scaffoldsmentioning

confidence: 99%

Computational Modelling of Wound Healing Insights to Develop New Treatments

Gómez‐Benito

Valero

García-Aznar

et al. 2019

New Developments in Tissue Engineering and Regeneration

View full text Add to dashboard Cite

About 1% of the population will suffer a severe wound during their life. Thus, it is really important to develop new techniques in order to properly treat these injuries due to the high socioeconomically impact they suppose. Skin substitutes and pressure based therapies are currently the most promising techniques to heal these injuries. Nevertheless, we are still far from finding a definitive skin substitute for the treatment of all chronic wounds. As a first step in developing new tissue engineering tools and treatment techniques for wound healing, in silico models could help in understanding the mechanisms and factors implicated in wound healing. Here, we review mathematical models of wound healing. These models include different tissue and cell types involved in healing, as well as biochemical and mechanical factors which determine this process. Special attention is paid to the contraction mechanism of cells as an answer to the tissue mechanical state. Other cell processes such as differentiation and proliferation are also included in the models together with extracellular matrix production. The results obtained show the dependency of the success of wound healing on tissue composition and the importance of the different biomechanical and biochemical factors. This could help to individuate the adequate concentration of growth factors to accelerate healing and also the best mechanical properties of the new skin substitute depending on the wound location in the body and

show abstract

A Snapshot of Direct Cell–Cell Communications in Wound Healing and Scarring

Cited by 28 publications

References 44 publications

Modulation of Foreign Body Reaction against PDMS Implant by Grafting Topographically Different Poly(acrylic acid) Micropatterns

Modulation of Foreign Body Reaction against PDMS Implant by Grafting Topographically Different Poly(acrylic acid) Micropatterns

Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomal MicroRNAs Suppress Myofibroblast Differentiation by Inhibiting the Transforming Growth Factor-β/SMAD2 Pathway During Wound Healing

Computational Modelling of Wound Healing Insights to Develop New Treatments

Contact Info

Product

Resources

About