A smart small molecule as specific fluorescent probe for sensitive recognition of mitochondrial DNA G-Quadruplexes

ON–OFF Fluorescent Cyanine Dye Based on a Benzothiophenyl Rotor Enables Selective Illumination of G-Quadruplexes in Mitochondria

“…The fluorescence intensity further increased alongside the incubation time and was saturated within 5 min. Compared to recently reported mitochondrial G4 probes, − our SN-Cy5-S probe enabled G4 imaging in a shorter time with a lower staining concentration (Table S5), indicating that the probe can quickly penetrate the cell membrane and fluoresce by binding to G4s in the cellular environment with an enhanced signal-to-noise ratio.…”

Structurally diverse G-quadruplexes as the noncanonical nucleic acid drug target for live cell imaging and antibacterial study

“…This special discrimination property of SPN may suggest a potential application for cancer diagnosis. 154 Furthermore, the cytotoxicity of SPN against HUVEC and HeLa cells was examined and the IC 50 was found to be higher than 20 μM regardless of short-term or long-term incubation with the cells. The result indicates that the ligand has moderate toxicity.…”

Two-Photon Nanoparticle Probe for Formaldehyde Detection via the AILE Luminescence Mechanism

“…23,24 Small molecule probes with high sensitivity and specificity. 25,26 However, detecting such probes depends on the design and response of the molecules, which rely on the reported luminous response mechanism [Forster resonance energy transfer, 27,28 intramolecular charge transfer (ICT), 29,30 photon-induced electron transfer, 31,32 aggregation induced emission, 33,34 and twisted intramolecular charge transfer 35,36 ], and the synthesis process is usually complex and has high toxicity. Therefore, developing a fluorescence molecular probe that can combine small molecules with large molecules via a new fluorescence response mechanism is an urgent problem that needs to be solved.…”

“…Fluorescent probes have the characteristics of high sensitivity and specificity and are mainly used for the detection of trace markers in vivo and vitro. − Generally, fluorescent probes can be divided into large molecular probes and small molecular probes. Macromolecular probes are mainly used in metal ion and bioimaging probes, which are easy to synthesize and have good biocompatibility. , However, this series of investigations has low sensitivity and a high defect detection line, which is unsuitable for in vitro diagnosis. , Small molecule probes with high sensitivity and specificity. , However, detecting such probes depends on the design and response of the molecules, which rely on the reported luminous response mechanism [Förster resonance energy transfer, , intramolecular charge transfer (ICT), , photon-induced electron transfer, , aggregation induced emission, , and twisted intramolecular charge transfer , ], and the synthesis process is usually complex and has high toxicity. Therefore, developing a fluorescence molecular probe that can combine small molecules with large molecules via a new fluorescence response mechanism is an urgent problem that needs to be solved.…”