A small volume, short-incubation-time method for measurement of photosynthesis as a function of incident irradiance

Lewis, Elfed; Smith, J. C.

doi:10.3354/meps013099

Cited by 414 publications

(278 citation statements)

References 11 publications

Supporting

Mentioning

277

Contrasting

Order By: Relevance

“…Chlorophyll a concentrations were determined on acetone-extracted Whatman GF/C filters (250 m1 filtrat i o n~) using the fluorometric method of Yentsch & Menzel (1963). Photosynthesis versus irradiance measurements were made on water samples from the first 3 depths, using the photosynthetron (Lewis & Smith 1983). Parameters aB, P : , , Ik and I, of the photosynthesis versus irradiance curves were estimated using the model of Platt et al (1980).…”

Section: Field Measurementsmentioning

confidence: 99%

An in vivo fluorescence method for the continuous in situ estimation of phytoplankton photosynthetic characteristics

Demers¹,

Therriault²,

Legendre³

et al. 1985

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

This study proposes a simple method to continuously measure, in situ, an in vivo fluorescence characteristic of a natural phytoplankton population, using 2 flow-through fluorometers and a strong light source. By this method, phytoplankton fluorescence before (F,) and after (F,) strong illumination (2250 ~E i n m-2 S-') was measured simultaneously with parameters of the photosynthesis vs irradiance curves. Data from 3 depths in the mixed layer, at 3 stations in the St. Lawrence Estuary, showed a strong relation between the ratio FA/FB and the photosynthetic parameters aB, Pi,,, I, and I, . The relation was particularly good with I, and I,, 2 possible indices of physiological adaptation. These results suggest that the ratio F,/FB can be used as an instantaneous in vivo index of photosynthetic characteristics. This simple measurement might prove useful for continuous spatio-temporal investigations of phytoplankton responses to their light environment in the water column.

show abstract

Section: Field Measurementsmentioning

confidence: 99%

An in vivo fluorescence method for the continuous in situ estimation of phytoplankton photosynthetic characteristics

Demers¹,

Therriault²,

Legendre³

et al. 1985

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…The cell suspensions were washed repeatedly with FSSW until a clear supernatant was obtained; final cell densities were adjusted to 3-9 x 1 O5 ml-l for use in experiments. Duplicate C fixation determinations were performed in 20-ml glass liquid scintillation vials (Lewis and Smith 1983).…”

Section: Methodsmentioning

confidence: 99%

Ammonium enhancement of dark carbon fixation and nitrogen limitation in symbiotic zooxanthellae: Effects of feeding and starvation of the sea anemone Aiptasia pallida

Cook¹,

Muller‐Parker

D’Elia³

1992

Limnology & Oceanography

View full text Add to dashboard Cite

The nutrient status of algae symbiotic with marine invertebrates is controversial. We assessed the nitrogen status of zooxanthellae symbiotic with the sea anemone Aiptasia pallida using NH,+ enhancement of dark C fixation; enhancement increases with N limitation in other microalgae. Freshly isolated symbionts obtained from laboratory populations of known feeding history and from field populations were assayed: NH,+ enhancement depended on the nutritional history of the host anemones. Zooxanthellae from well-fed laboratory populations showed little enhancement of dark 14C fixation by NH,+, while mean dark enhancement ratios (NH,' rates: seawater rates) exceeded 2.3 for zooxanthellae from anemones unfed for l-6 weeks. Photosynthetic rates of the isolated algae declined with starvation. V,,, : V,, an index which includes both dark NH,+ enhancement and photosynthesis, increased markedly with time since feeding. Three of four field samples of A. pallida yielded zooxanthellae with dark enhancement ratios similar to those of fed laboratory populations; I',. : V, values were somewhat higher. Symbionts from anemones collected on a fourth date had a mean enhancement ratio of 2.88, and I',,. : V, values similar to those from laboratory anemones that had not fed for l-2 weeks, indicative of increased N limitation.

show abstract

“…Both instruments were mounted on the frame of the CTD. Primary production estimates and photosynthetic parameters were determined using a small-bottle •4C technique modified from Lewis and Smith [1983] and described by Mackey et al [1995]. Usually, six depths per station between the surface and 100 m were sampled using Niskin bottles fitted with silicon rubber O-rings and tubing.…”

Section: Sampling On Rv Discoverermentioning

confidence: 99%

Oceanographic context of the First Aerosol Characterization Experiment (ACE 1): A physical, chemical, and biological overview

Griffiths¹,

Bates²,

Quinn³

et al. 1999

J. Geophys. Res.

View full text Add to dashboard Cite

A small volume, short-incubation-time method for measurement of photosynthesis as a function of incident irradiance

Cited by 414 publications

References 11 publications

An in vivo fluorescence method for the continuous in situ estimation of phytoplankton photosynthetic characteristics

An in vivo fluorescence method for the continuous in situ estimation of phytoplankton photosynthetic characteristics

Ammonium enhancement of dark carbon fixation and nitrogen limitation in symbiotic zooxanthellae: Effects of feeding and starvation of the sea anemone Aiptasia pallida

Oceanographic context of the First Aerosol Characterization Experiment (ACE 1): A physical, chemical, and biological overview

Contact Info

Product

Resources

About