A Small Peptide with Potential Ability to Promote Wound Healing

Abstract

Wound-healing represents a major health burden, such as diabetes-induced skin ulcers and burning. Many works are being tried to find ideal clinical wound-healing biomaterials. Especially, small molecules with low cost and function to promote production of endogenous wound healing agents (i.e. transforming growth factor beta, TGF-β) are excellent candidates. In this study, a small peptide (tiger17, c[WCKPKPKPRCH-NH2]) containing only 11 amino acid residues was designed and proved to be a potent wound healer. It showed strong wound healing-promoting activity in a murine model of full thickness dermal wound. Tiger17 exerted significant effects on three stages of wound healing progresses including (1) the induction of macrophages recruitment to wound site at inflammatory reaction stage; (2) the promotion of the migration and proliferation both keratinocytes and fibroblasts, leading to reepithelialization and granulation tissue formation; and (3) tissue remodeling phase, by promoting the release of transforming TGF-β1 and interleukin 6 (IL-6) in murine macrophages and activating mitogen-activated protein kinases (MAPK) signaling pathways. Considering its easy production, store and transfer and function to promote production of endogenous wound healing agents (TGF-β), tiger17 might be an exciting biomaterial or template for the development of novel wound-healing agents.

Antimicrobial endotoxin‐neutralizing peptides promote keratinocyte migration via P2X7 receptor activation and accelerate wound healing in vivo

“…Additionally, to promotion of cell migration in vitro , AMPs support wound healing in vivo by sustaining wound re‐epithelialization. The small peptide tiger 17, which promoted keratinocyte migration as well as proliferation in vitro , was capable of improving re‐epithelialization in vivo (Tang et al ., ). AH90, a peptide from frog skin, accelerated wound closure in vivo in non‐infected wounds and improved epidermal and dermal regeneration and granulation tissue formation (Liu et al ., ).…”

Cox-2 inhibition potentiates mouse bone marrow stem cell engraftment and differentiation-mediated wound repair

“…The rate of wound healing was represented as percentage of wound closure [(wound area of original wound-area of remaining wound)/wound area of original wound × 100] as described earlier [18].…”

Effects of Sidr honey on second-intention healing of contaminated full-thickness skin wounds in healthy dogs

“…Treatment of wounds using topically applied medications is a common therapeutic approach (22,20,25). The use of honey as a topical treatment of wounds or as a wound dressing was found to enhance healing (1).…”