A small non-coding RNA facilitates Brucella melitensis intracellular survival by regulating the expression of virulence factor

Wang, Yufei; Yuan, Ke; Duan, Cuijuan; Ma, Xueping; Hao, Qinfang; Song, Lijie; Guo, Xiaojie; Sun, Tao; Zhang, Wei; Zhang, Jing; Zhao, Yichao; Zhong, Zhijun; Yang, Xiaoli; Chen, Zeliang

doi:10.1016/j.ijmm.2019.04.002

Cited by 14 publications

(9 citation statements)

References 35 publications

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“…Studies on another bacterial strain, Brucella melitensis, provided evidence that Bsr1141 (75 nt), an abundant Hfq-dependent sRNA, modulates the expression of virB2 gene, which is one of the 12 genes (virB1-12) of the VirB type IV secretion (T4SS) of Brucella. Thanks to Bsr1141 Brucella could withstand different environmental conditions during their long-term residence in host macrophages [91]. The major virulence factors reported in Brucella pathogenesis are often lipopolysaccharide (LPS), T4SS, and the BvrR/BvrS system [92,93].…”

Section: Gram-negative Bacteriamentioning

confidence: 99%

RNA-Sequencing Analyses of Small Bacterial RNAs and their Emergence as Virulence Factors in Host-Pathogen Interactions

Diallo

Provost

2020

IJMS

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Proteins have long been considered to be the most prominent factors regulating so-called invasive genes involved in host-pathogen interactions. The possible role of small non-coding RNAs (sRNAs), either intracellular, secreted or packaged in outer membrane vesicles (OMVs), remained unclear until recently. The advent of high-throughput RNA-sequencing (RNA-seq) techniques has accelerated sRNA discovery. RNA-seq radically changed the paradigm on bacterial virulence and pathogenicity to the point that sRNAs are emerging as an important, distinct class of virulence factors in both gram-positive and gram-negative bacteria. The potential of OMVs, as protectors and carriers of these functional, gene regulatory sRNAs between cells, has also provided an additional layer of complexity to the dynamic host-pathogen relationship. Using a non-exhaustive approach and through examples, this review aims to discuss the involvement of sRNAs, either free or loaded in OMVs, in the mechanisms of virulence and pathogenicity during bacterial infection. We provide a brief overview of sRNA origin and importance and describe the classical and more recent methods of identification that have enabled their discovery, with an emphasis on the theoretical lower limit of RNA sizes considered for RNA sequencing and bioinformatics analyses.

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Section: Gram-negative Bacteriamentioning

confidence: 99%

RNA-Sequencing Analyses of Small Bacterial RNAs and their Emergence as Virulence Factors in Host-Pathogen Interactions

Diallo

Provost

2020

IJMS

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“…The bacteria solution was sonicated three times on ice using a high intensity ultrasonic processor in lysis buffer (8 M urea, 1% protease Inhibitor Cocktail) (protease Inhibitor Cocktail, Beyotime, China, P1005). The remaining cell debris was removed by centrifugation at 12,000 g at 4°C for 10 min ( Wang et al, 2019 ). The supernatant was collected, and the protein concentration was determined using a BCA kit (Beyotime, China, P0012) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Lysine Acylation Modification Landscape of Brucella abortus Proteome and its Virulent Proteins

Zhang

Chen

Dong

et al. 2022

Front. Cell Dev. Biol.

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The myriad of posttranslational modifications (PTMs) of proteins that occur in all living cells are crucial to all kinds of biological processes. Brucella is an intracellular parasitic bacterium that can cause chronic diseases in both humans and livestock. To reveal the relationship between PTMs and the virulence and survival of Brucella, we described the first comprehensive multiple PTM-omics atlas of B. abortus 2308. Five PTMs involving lysine, namely 2-hydroxyisobutyrylation, succinylation, crotonylation, acetylation, and malonylation were identified. Nearly 2,000 modified proteins were observed, and these proteins took part in many biological processes, with a variety of molecular functions. In addition, we detected many significant virulence factors of Brucella among the modified proteins. 10 of the 15 T4SS effector proteins were detected with one or more PTMs. Moreover, abundant PTMs were detected in other typical virulence factors. Considering the role of PTMs in various biological processes of Brucella virulence and survival, we propose that the virulence of Brucella is associated with the PTMs of proteins. Taken together, this study provides the first global survey of PTMs in Brucella. This is a prospective starting point for further functional analysis of PTMs during the survival of Brucella in hosts, interpretation of the function of Brucella proteins, and elucidation of the pathogenic mechanism of Brucella.

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“…The GEM medium was used for starvation treatment of B. abortus. The components of the GEM medium are as follows: MgSO 4 7H 2 O (0.2 g/L), citric acidH 2 O (2.0 g/L), K 2 HPO 4 (10.0 g/L), NaNH 4 HPO 4 4H 2 O (3.5 g/L), and glucose (20 g/L; Wang et al, 2019). The cells harvested from the exponential or stationary growth stage were resuspended in the GEM medium and then incubated at 37 • C by shaking the incubator for 1.5 h. The pH values of all media in this study were adjusted to 7.0.…”

Section: Brucella Strains and Experimental Designmentioning

confidence: 99%

Proteomic Analysis of Stationary Growth Stage Adaptation and Nutritional Deficiency Response of Brucella abortus

Yang

Liu²,

Liu³

et al. 2020

Front. Microbiol.

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Brucellosis, an important bacterial zoonosis caused by Brucella species, has drawn increasing attention worldwide. As an intracellular pathogen, the ability of Brucella to deal with stress within the host cell is closely related to its virulence. Due to the similarity between the survival pressure on Brucella within host cells and that during the stationary phase, a label-free proteomics approach was used to study the adaptive response of Brucella abortus in the stationary stage to reveal the possible intracellular adaptation mechanism in this study. A total of 182 downregulated and 140 upregulated proteins were found in the stationary-phase B. abortus. B. abortus adapted to adverse environmental changes by regulating virulence, reproduction, transcription, translation, stress response, and energy production. In addition, both exponential- and stationary-phase B. abortus were treated with short-term starvation. The exponential B. abortus restricted cell reproduction and energy utilization and enhanced material transport in response to nutritional stress. Compared with the exponential phase, stationary Brucella adjusted their protein expression to a lesser extent under starvation. Therefore, B. abortus in the two growth stages significantly differed in the regulation of protein expression in response to the same stress. Overall, we outlined the adaptive mechanisms that B. abortus may employ during growth and compared the differences between exponential- and stationary-phase B. abortus in response to starvation.

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A small non-coding RNA facilitates Brucella melitensis intracellular survival by regulating the expression of virulence factor

Cited by 14 publications

References 35 publications

RNA-Sequencing Analyses of Small Bacterial RNAs and their Emergence as Virulence Factors in Host-Pathogen Interactions

RNA-Sequencing Analyses of Small Bacterial RNAs and their Emergence as Virulence Factors in Host-Pathogen Interactions

Lysine Acylation Modification Landscape of Brucella abortus Proteome and its Virulent Proteins

Proteomic Analysis of Stationary Growth Stage Adaptation and Nutritional Deficiency Response of Brucella abortus

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