A small indel mutation in an anthocyanin transporter causes variegated colouration of peach flowers

Cheng, Jun; Liao, Liao; Zhou, Hui; Gu, Chao; Wang, Lu; Han, Yuepeng

doi:10.1093/jxb/erv419

Cited by 77 publications

(66 citation statements)

References 72 publications

(102 reference statements)

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“…The involvement of GSTs in trafficking and accumulation of anthocyanins have been verified in maize (Marrs et al, 1995), petunia (Mueller et al, 2000), Arabidopsis (Sun et al, 2012), cyclamen (Kitamura et al, 2012), perilla , grape (Conn et al, 2008), apple (Jiang et al, 2019) and litchi (Hu et al, 2016). In this study, we found a GST (PpGST1), with GeneID (Prupe.3G013600, also previously as ppa011307m) later found to be identical to that of an anthocyanin transporter (Riant) related to the variegated coloration of peach flowers (Cheng et al, 2015), was differentially expressed in 'HJ' and 'YL' throughout developmental stages, which may account for the distinct anthocyanin accumulation in the fruit of two cultivars. Also, the transcription of PpGST1 was consistent with anthocyanin contents of peel and flesh in different cultivars.…”

Section: Discussionsupporting

confidence: 69%

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

Zhao

Dong

Zhu

et al. 2019

Plant Biotechnology Journal

118

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Summary Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar ‘Hujingmilu’ and lightly pigmented cultivar ‘Yulu’. The difference was not fully explained by accumulation of total flavonoids and expression of anthocyanin biosynthetic genes. Expression analysis was conducted on a glutathione S‐transferase gene (PpGST1), and it was found that the expression correlated well with anthocyanin accumulation in peach fruit tissues. Functional complementation of the Arabidopsis tt19 mutant indicated that PpGST1 was responsible for transport of anthocyanins but not proanthocyanidins. PpGST1 was localized in nuclei and the tonoplast, including the sites at which anthocyanin vacuolar sequestration occurred. Transient overexpression of PpGST1 together with PpMYB10.1 in tobacco leaves and peach fruit significantly increased anthocyanin accumulation as compared with PpMYB10.1 alone. Furthermore, virus‐induced gene silencing of PpGST1 in a blood‐fleshed peach not only resulted in a reduction in anthocyanin accumulation but also a decline in expression of anthocyanin biosynthetic and regulatory genes. Cis‐element analysis of the PpGST1 promoter revealed the presence of four MYB binding sites (MBSs). Dual‐luciferase assays indicated that PpMYB10.1 bound to the promoter and activated the transcription of PpGST1 by recognizing MBS1, the one closest to the ATG start codon, with this trans‐activation being stronger against the promoter of deep coloured ‘Hujingmilu’ compared with lightly coloured cultivar ‘Yulu’. Altogether, our data provided molecular evidence supporting coordinative regulatory roles of PpGST1 and PpMYB10.1 in anthocyanin accumulation in peach.

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Section: Discussionsupporting

confidence: 69%

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

Zhao

Dong

Zhu

et al. 2019

Plant Biotechnology Journal

118

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“…This suggests that PpUFGT encodes a rate-limiting enzyme that determines the overall rate of anthocyanin biosynthesis, which supports the previous finding of PpMYB10.1 activating UFGT in tobacco 57 . Besides PpUFGT, an anthocyanin transporter gene PpGST was also significantly activated, which is consistent with previous reports that GST acts downstream of anthocyanin-related MYB gene MYB10.1 59,60 . These results demonstrate that our newly developed transgenic root system has applications in functional characterization of genes and genetic engineering in peach or its related fruit trees of the Rosaceae family.…”

Section: Discussionsupporting

confidence: 92%

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

Lai

Zhao

et al. 2020

Sci Rep

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Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated transformation system in peach. Various explants, including leaf, hypocotyl and shoot, were all able to induce transgenic hairy roots, with a transformation efficiency of over 50% for hypocotyl. Composite plants were generated by infecting shoots with A. rhizogenes to induce transgenic adventitious hairy roots. The composite plant system was successfully used to validate function of an anthocyanin-related regulatory gene PpMYB10.1 in transgenic hairy roots, and two downstream genes, PpUFGT and PpGST, were strongly activated. Our stable and reproductive A. rhizogenes-mediated transformation system provides an avenue for gene function assay, genetic engineering, and investigation of root-rhizosphere microorganism interaction in peach. open Scientific RepoRtS | (2020) 10:2836 | https://doi.

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“…A TT2-like R2R3-MYB gene, designated Peace , regulates petal pigmentation in flowering peach ‘Genpei’ bearing variegated and fully pigmented flowers although it is unclear whether the Peace gene is able to activate anthocyanin and/or proanthocyanin pathway structural genes ( Uematsu et al, 2014 ). However, our study shows that a small indel mutation in an anthocyanin transporter, which is not the result of a transposon insertion or excision, causes variegated colouration of flowers of ornamental peach, ‘Hongbaihuatao’ ( Cheng et al, 2015 ). These results suggest a complex mechanism regulating anthocyanin accumulation in peach flower.…”

Section: Introductionmentioning

confidence: 71%

Multiple R2R3-MYB Transcription Factors Involved in the Regulation of Anthocyanin Accumulation in Peach Flower

Zhou¹,

Peng²,

Zhao³

et al. 2016

Front. Plant Sci.

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Anthocyanin accumulation is responsible for flower coloration in peach. Here, we report the identification and functional characterization of eight flavonoid-related R2R3-MYB transcription factors, designated PpMYB10.2, PpMYB9, PpMYBPA1, Peace, PpMYB17, PpMYB18, PpMYB19, and PpMYB20, respectively, in peach flower transcriptome. PpMYB10.2 and PpMYB9 are able to activate transcription of anthocyanin biosynthetic genes, whilst PpMYBPA1 and Peace have a strong activation on the promoters of proanthocyanin (PA) biosynthetic genes. PpMYB17-20 show a strong repressive effect on transcription of flavonoid pathway genes such as dihydroflavonol 4-reductase. These results indicate that anthocyanin accumulation in peach flower is coordinately regulated by a set of R2R3-MYB genes. In addition, PpMYB9 and PpMYB10.2 are closely related but separated into two groups, designated MYB9 and MYB10, respectively. PpMYB9 shows a strong activation on the PpUGT78A2 promoter, but with no effect on the promoter of PpUGT78B (commonly called PpUFGT in previous studies). In contrast, PpMYB10.2 is able to activate the PpUFGT promoter, but not for the PpUGT78A2 promoter. Unlike the MYB10 gene that is universally present in plants, the MYB9 gene is lost in most dicot species. Therefore, the PpMYB9 gene represents a novel group of anthocyanin-related MYB activators, which may have diverged in function from the MYB10 genes. Our study will aid in understanding the complex mechanism regulating floral pigmentation in peach and functional divergence of the R2R3-MYB gene family in plants.

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A small indel mutation in an anthocyanin transporter causes variegated colouration of peach flowers

Abstract: HighlightAn anthocyanin transporter gene that contains a high frequency of non-transposable element-related mutations is responsible for variegated colouration of flowers in peach.

Cited by 77 publications

References 72 publications

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach

Development of a fast and efficient root transgenic system for functional genomics and genetic engineering in peach

Multiple R2R3-MYB Transcription Factors Involved in the Regulation of Anthocyanin Accumulation in Peach Flower

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