A Skin‐graft Enhancing Antiserum Which Recognizes Two New B‐cell Alloantigens Determined by the Major Histo‐compatibility Locus of the Mouse

Abstract

SUMMARY A mouse alloantiserum, raised by immunizing B10.D2/n mice with B10.M spleen, has been found to retain cytotoxic activity against a proportion of lymph node cells even after exhaustive absorption with B10.M red blood cells. Titration and absorption with a number of strains showed that the absorbed serum retained antibodies against two new specificities. The absorbed serum gave significant enhancement of B10.M skin grafts on B10.D2 and reacted mainly with B cells.

The dominant role of la antibodies in the passive enhancement of H‐2 incompatible skin grafts

“…Titration against B10.D2 lymph node cells of BIO.A anti-B10.D2 antisera (S117) raised against different cell types. Individual curves represent the activity of serum pools from groups of mice that all received two sequential skin grafts followed by one of the following: spleen cells ( 0 ) ; thymus cells (=); whole blood (v); blood leukocytes (0); purified RBC (12); purified blood platelets ( X I ) . a) Antisera (S117), illustrated also in Fig.…”

The Detection of Ia Antibodies in Polyspecific H‐2 Alloantisera Absorbed with Erythrocytes

“…H-2. 58, 59 have Ia like properties and are not expressed on RBC (Archer et al 1974), but 59 and Ia.3 are probably absent because of no reaction against the same H-2b and H-2' 4 cells. The serum thus We have noted that H-2' -+ H-2d sera frequently react with H-2' lymphocytes with a characteristically low release or biphasic cytotoxicity profile.…”

“…The serum thus We have noted that H-2' -+ H-2d sera frequently react with H-2' lymphocytes with a characteristically low release or biphasic cytotoxicity profile. This reaction was assumed to be due to H-2.45 and, of course, may now involve the Ia-like specificities 58 and 59 (Archer et al 1974). An anti-H-2' serum (S69) was absorbed with I/St RBC.…”

“…Following the findings of Davies & Alkins (1974) that Ia-like, erythrocyte-absorbed Ag-B sera would enhance auxiiliary heart grafts in the rat, we examined the specificity of antisera which passively enhance the survival of skin allografts in the mouse and have found that enhancing antisera can be absorbed with red blood cells (RBC) to remove the K and D antibodies without changing their enhancing efficiency , Archer et al 1974. Such absorbed antisera contain residual cytotoxic antibodies having all the serological properties of Ia antibodies.…”

Tich: a mutant causing disproportional growth in the mouse

“…The presence of H-2" was sought using serum A175 (A.SW anti-B10.BR spleen-enlarging lymphoma; H-2' anti-H-2 k ) by a standard complement-mediated 51 Cr-release cytotoxicity test (Archer et al 1974). This combination assumes that antibodies to the H-2" class II region, derived from both A.TL and C3H, are distinguishable in the backcross from H-2 k class I (C3H only).…”