A single-step O-glycosylation of azidothymidine in bioreactor catalysed by filamentous fungi

Arruda, Evilanna Lima; Nunes, Elaine S.; Souza, Paula Letícia de Melo; Japiassu, Kamila Bohne; Carvalho, Thays Colletes de; Vaz, Boniek G.; Lião, Luciano M.; Oliveira, Valéria de

doi:10.1016/j.tetlet.2016.08.050

Cited by 4 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we observed that the percentage of mixed cell count (%MXD) remained relatively stable across the various treatment groups. This finding aligns with the previous study conducted by (40), further corroborating the consistency of our results.…”

Section: Figuresupporting

confidence: 94%

See 1 more Smart Citation

O3fa Alleviates Inflammation in Challenged Animal Models and Exerts Inhibitory Effect on Leukocyte Differential Parameters in Carrageenan-Induced Peritonitis

Omale,

Egu

2024

Preprint

View full text Add to dashboard Cite

Introduction : Anti-inflammatory action of dietary omega-3-polyunsaturated fatty acid (O3FA) supplementation in animal models was studied using standard methods. The therapeutic safety of anti-inflammatory supplements utilizing natural forms, such as omega -3 fatty acids (n-3 fatty acids), is important in addition to its effectiveness. Methods: The nutritional intervention of omega-3 fish oil extract in experimental animals for acute inflammatory models was investigated using standard methods (Carrageenan, Arachidonic acid, and Prostaglandin E2 analyses). Total leukocyte count and differential parameters in carrageenan-induced peritonitis were also determined by standard methods. Results: Results obtained demonstrated a notable reduction in inflammation caused by the O3FA. For groups 1, 2, 3, 4, and 5, the percentage inhibition of the carrageenan-induced paw oedema after 24 hours was 78.57 %, 57.14 %, 50.00 %, 100.00 % and 92.85 %, respectively. Arachidonic acid-induced ear oedema percentage inhibition was 30, 24, 27, 37, and 40 %, while the PGE-2 test showed inhibition of by 44.16, 52.48, 61.34, 74, and 80.05 %. The novelty of the work is demonstrated in the use of dietary supplements as anti- inflammatory compounds as opposed to the usual non- steroidal anti-inflammatories. The results also show O3FA exerting an inhibitory effect on leukocyte parameters. As the dose of the extract increased, there was a trend of decreasing total leukocyte count. The groups administered with a 20mg dose of celecoxib (5.6 ± 0.23 x 10 3) and a combination of 300mg O3FA with 20mg celecoxib (5.9 ± 0.18 x 10 3) exhibited the lowest overall leukocyte count, notably lacking statistically significant variance. Conclusion: This outcome underscores the extract's heightened inhibitory impact on leukocyte migration amid peritonitis. Inhibition of carrageenan-induced peritonitis was 26.47, 18.63, 30.39, 42.16 and 45.09 %. The control group showed an ANC of 6.74 x 10 3/µL, an ALC of 3.09 x 10 3/µL, and an AMXD of 0.37 x 10 3/µL. Absolute Neutrophil Count (ANC) for all the treatment groups fall between 3080 - 6740µl which is within the acceptable limit of 2500 - 7000µl for ANC. Also, for ALC, which is 2300 - 3090µl from the table has an acceptable limit of 1000 - 5000µl while that for MXD from the table is from 220 - 370µl which is well within the limit of 200 – 1090µl acceptability.

show abstract

Section: Figuresupporting

confidence: 94%

“…The differential leukocyte count analysis demonstrates the extract's ability to decrease neutrophil percentage while promoting a slight increase in lymphocyte percentage. Additionally, the consistent percentage of mixed cell count across the different groups further strengthens the validity of our findings, consistent with the work of (40).…”

Section: Figuresupporting

confidence: 89%

O3fa Alleviates Inflammation in Challenged Animal Models and Exerts Inhibitory Effect on Leukocyte Differential Parameters in Carrageenan-Induced Peritonitis

Omale,

Egu

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The condensation reaction between À COOH and À OH is extensively employed for the synthesis of protein macromolecules or large steric hindrance molecules. [18] For instance, Yang et al reviewed in detail the synthesis of novel active esters from carboxylic acids and nucleophilic hydroxyl components in the presence of activators for protein synthesis. In addition, the synthesis of biologically active macrolides with large steric hindrance involves the use of carbon diimide condensation reagents.…”

Section: Introductionmentioning

confidence: 99%

−COOH & −OH Condensation Reaction Utilization for Biomass FDCA‐based Polyesters

Yi,

Dai,

et al. 2024

ChemSusChem

View full text Add to dashboard Cite

A green and sustainable ‐COOH & ‐OH condensation solution polymerization method was hereby reported for FDCA‐based polyesters to avoid discoloration and toxic solvents. First, taking poly(ethylene 2,5‐furandicarboxylate) (PEF) as the representative of FDCA‐based polyester, enabling good white appearance PEF with Mn = 6.51×103 g mol‐1 from FDCA and ethylene glycol in green solvent γ‐valerolactone (GVL), catalyzed by 4‐dimethylaminopyridine (DMAP) and N‐(3‐dimethylaminopropyl)‐N’‐ethylcarbodiimide hydrochloride (EDC). Additionally, the molecular weight of PEF was rapidly improved (Mn > 2.5 × 104 g mol‐1) via remelting polycondensation within minutes, with the dispersity still kept relatively low dispersity (Đ <1.40). Importantly, the ‐COOH & ‐OH condensation solution polymerization method was successfully applied for the synthesis of various FDCA‐based polyesters, including diols with varying carbon chain lengths (3 to 11 carbons) and cycloalkyl diols, especially the applicability of this method to diols containing C=C double bonds, which was found to exhibit low heat resistance. Lastly, assisting with 13C labeled 1,4‐succinic acid and in‐situ 13C‐NMR, an in‐depth study of the possible catalytic mechanism was proposed, by which, EDC activated FDCA, and then DMAP catalyzed it with diol to yield macromolecular chain of polyester. Overall, the results provided a green and sustainable strategy for the synthesis of FDCA‐based polyesters.

show abstract

Stereoselective biosynthesis of 3′-azido-3′-deoxythymidine 5′-O-β-D-ribopyranoside and in vitro evaluation as potential antileishmanial with in silicoADME prediction

Arruda

Nunes

Japiassu

et al. 2019

Process Biochemistry

View full text Add to dashboard Cite

A single-step O-glycosylation of azidothymidine in bioreactor catalysed by filamentous fungi

Cited by 4 publications

References 17 publications

O3fa Alleviates Inflammation in Challenged Animal Models and Exerts Inhibitory Effect on Leukocyte Differential Parameters in Carrageenan-Induced Peritonitis

O3fa Alleviates Inflammation in Challenged Animal Models and Exerts Inhibitory Effect on Leukocyte Differential Parameters in Carrageenan-Induced Peritonitis

−COOH & −OH Condensation Reaction Utilization for Biomass FDCA‐based Polyesters

Stereoselective biosynthesis of 3′-azido-3′-deoxythymidine 5′-O-β-D-ribopyranoside and in vitro evaluation as potential antileishmanial with in silicoADME prediction

Contact Info

Product

Resources

About