A single-step DNAzyme sensor for ultra-sensitive and rapid detection of Pb2+ ions

Meng, Jiyu; Huang, Jiamei; Oueslati, Rania; Jiang, Yanting; Chen, Jiangang; Li, Shanshan; Dai, Shijie; He, Qiang; Cheng, Cheng

doi:10.1016/j.electacta.2020.137551

Cited by 24 publications

(10 citation statements)

References 51 publications

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“…At a higher frequency, ACET bulk electroconvection becomes obvious in the bulk fluid caused by Maxwell–Wagner structural polarization . Electric properties of separation buffer, involving conductivity and dielectric permittivity, will have an evident response for varying local temperatures , as a result of Joule heating in the buffer. A dielectric gradient generates in the buffer, giving rise to a volume force on the bulk liquid , where T indicates the temperature, and σ( T ) and ε( T ) are the fluid conductivity and permittivity at different temperatures, respectively.…”

Section: Theory Backgroundmentioning

confidence: 99%

Dielectric Characterization and Multistage Separation of Various Cells via Dielectrophoresis in a Bipolar Electrode Arrayed Device

et al. 2021

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Isolation of microalgal cells is as an indispensable part of producing biofuels for energy security and detecting toxic contaminants for marine routine monitoring. Microalgae live together with various microalgae naturally, and abundant samples need to be tackled in practical applications. Therefore, effective separation technologies need to be developed urgently to achieve high-throughput separation of various microalgae. Herein, we develop a reliable device to characterize the dielectric response of microalgae and sequentially separate various microalgae utilizing dielectrophoretic force in a bipolar electrode (BPE) arrayed device. First, by investigating the array width extension (AWE) effect on the electric- and flow-field distributions, we explore consequences of incidental electrohydrodynamic mechanisms and axial flow rate on the separation. Second, based on device performance on sample characterizations, we demonstrate this technology by separating microparticles in three- and five-channel devices. Third, we discriminate dead and live cells to explore its capability using the cell viability test and illustrate the AWE influence on the separation. Fourth, we characterize dielectric responses of different microalgae and separate C. vulgaris and Oocystis sp. Finally, we extended BPEs in length and developed an arrayed device for sequential separation of various microalgae, and this platform is successfully engineered in high-throughput isolation of C. vulgaris from complex samples. This technology presents good potential in addressing depleting fossil fuel and burgeoning environmental concerns due to its performance in the separation of microalgal strains from complex samples.

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Section: Theory Backgroundmentioning

confidence: 99%

Dielectric Characterization and Multistage Separation of Various Cells via Dielectrophoresis in a Bipolar Electrode Arrayed Device

et al. 2021

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“…To overcome these limitations for detection, ACEK effects were applied for molecule enrichment. Specifically, ACEK effects include dielectrophoresis (DEP) [23], AC electroosmosis (ACEO) [24] and AC electrothermal (ACET) effect [25]. For ACEO, the flow velocity diminished to close to zero when the conductivity of solution was increased to above 0.085 S/m [26].…”

Section: Acek Enrichment Mechanismmentioning

confidence: 99%

“…Because both were larger than the miRNA-16b tested here, the DEP effect was much more pronounced for enrichment. Thereby, it was reasonable to have a better response at 10 kHz for miRNA-16b detection due to the dominance of the ACET effect for miRNA enrichment [24,31].…”

Section: Frequency Optimizationmentioning

confidence: 99%

Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Wan

Jiang

Huang

et al. 2021

Sensors

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A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

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“…In the presence of the cation, the substrate is cut at the cleavage site (deoxyguanosine) and the fragments are released, leaving a part of the substrate strand immobilized on the sensor. In this regard, DNAzymes were exploited for fluorescent and electrochemical sensors for the determination of Pb(II) [9,28,45,63,158,166,167,[172][173][174][175]179,180], Cu(II) [133], Cd(II) [126], Hg(II) [40,135,138], Mg(II) [42], and Ni(II) [157]. In these procedures, substrate strands were labeled with electrochemical and fluorescent probes that showed responses after their disassembly from the platform.…”

Section: Affinitymentioning

confidence: 99%

An overview of Structured Biosensors for Metal Ions Determination

et al. 2021

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The determination of metal ions is important for nutritional and toxicological assessment. Atomic spectrometric techniques are highly efficient for the determination of these species, but the high costs of acquisition and maintenance hinder the application of these techniques. Inexpensive alternatives for metallic element determination are based on dedicated biosensors. These devices mimic biological systems and convert biochemical processes into physical outputs and can be used for the sensitive and selective determination of chemical species such as cations. In this work, an overview of the proposed biosensors for metal ions determination was carried out considering the last 15 years of publications. Statistical data on the applications, response mechanisms, instrumentation designs, applications of nanomaterials, and multielement analysis are herein discussed.

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A single-step DNAzyme sensor for ultra-sensitive and rapid detection of Pb2+ ions

Cited by 24 publications

References 51 publications

Dielectric Characterization and Multistage Separation of Various Cells via Dielectrophoresis in a Bipolar Electrode Arrayed Device

Dielectric Characterization and Multistage Separation of Various Cells via Dielectrophoresis in a Bipolar Electrode Arrayed Device

Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

An overview of Structured Biosensors for Metal Ions Determination

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