A single quantum dot-based biosensor for telomerase assay

Abstract

Telomerase is a promising biomarker and a therapeutic target due to its extensive expression in human tumors such as lung cancer and breast cancer. Here, we develop a single quantum dot (QD)-based biosensor for the sensitive detection of telomerase activity. This single QD-based biosensor has significant advantages of simplicity and high sensitivity, and it can be applied for the discrimination of tumor cells from normal cells as well as the screening of anticancer drugs.

Advances in optical assays for detecting telomerase activity

“…Therefore, the enhanced FRET was utilized for sensitive detection of telomerase activity. In 2015, Zhang and coworkers developed a QD‐based FRET platform (Figure ) . In the presence of telomerase, the TEP strand was assembled with multiple Cy5 molecules by using Cy5‐dATP.…”

“…Schematic illustration of a single quantum dot (QD)‐based biosensor for telomerase assay. Reprinted with permission from ref …”

An AIEgens and exonuclease III aided quadratic amplification assay for detecting and cellular imaging of telomerase activity

“…As an alternative to PCR, isothermal amplification based telomerase detection strategies which avert the PCR-induced problems and demand of gel analysis in TRAP assay have been developed in recent years [27][28][29][30][31][32][33][34]. Among them, florescence biosensors have been widely applied in telomerase activity detection, owing to their nature virtues of simple operation, high sensitivity, small volume of samples and easy reading [35][36][37][38][39][40][41][42][43][44][45][46][47].…”

Synthesis and multifunctional applications of quantum nanobeads for label-free and selective metal chemosensing

“…2 So a literature survey showed various applications of QDs for chemosensing and biosensing to detect multifarious analytes. They possess unique features, that are absent in many other nanoparticles, such as their unique size-dependent, optical and electronic properties.…”