A single polypeptide catalyzing the conversion of phytoene to zeta-carotene is transcriptionally regulated during tomato fruit ripening.

Pecker, Iris; Chamovitz, Daniel; Linden, Hartmut; Sandmann, Gerhard; Hirschberg, Joseph

doi:10.1073/pnas.89.11.4962

Cited by 183 publications

(168 citation statements)

References 24 publications

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“…Genes encoding this protein have been identified and obtained from plants like Arabidopsis, tomato, pepper, maize, daffodil, soybean, tobacco and some others [56,61,[77][78][79][80][81].…”

Section: Plantsmentioning

confidence: 99%

A comprehensive review on the colorless carotenoids phytoene and phytofluene

Meléndez-Martínez

Benítez-González

Stinco

2015

Archives of Biochemistry and Biophysics

152

112

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“…Genes encoding this protein have been identified and obtained from plants like Arabidopsis, tomato, pepper, maize, daffodil, soybean, tobacco and some others [56,61,[77][78][79][80][81].…”

Section: Plantsmentioning

confidence: 99%

A comprehensive review on the colorless carotenoids phytoene and phytofluene

Meléndez-Martínez

Benítez-González

Stinco

2015

Archives of Biochemistry and Biophysics

152

112

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“…First, CRT-I type of phytoene desaturases have been cloned from the bacteria Rhodobacter capsulatus [13], Erwinia uredovora and E. herbicola (crtl genes) [14,15] and from the fungus Neurospora crassa (aLl gene) [16] and sequenced. Then, the genes encoding phytoene desaturases from cyanobacteria [12,17], green algae and plants [11,18,19] have been isolated (pds genes). Sequence data indicate that the CRT-I type of desaturases and the PDS-type of desaturases belong to 2 separate groups of enzymes.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional expression in E. coli of a novel plant enzyme mediating ξ‐carotene desaturation

et al. 1995

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~bstract We have cloned a cDNA from the plant Capsicum annuum which encodes a novel enzyme mediating the dehydrogenation of ~-carotene and neurosporene to lycopene when expressed in E. coli cells accumulating ~-carotene or neurosporene. this enzyme is unable to dehydrogenate either phytoene or lycopene. The deduced amino acid sequence suggests that this cDNA encodes a polypeptide whose mature size is ca. 59 kDa and which is synthesized as a precursor with a NH2-terminal extension resembling transit peptides for plastid targeting. Sequence comparison reveals 33-35% similarity with previously cloned plant or cyanobacterial phytoene desaturases. In contrast, only limited sequence similarity is found with a ~-carotene desaturase from the cyanobacterium Anabaena.

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“…19. Blots were hybridized with dCTP-labeled gene fragments, crtB BamHI fragments from pRN2, the 1.6-kb pTOM5 cDNA (20), the 1.3-kb EcoRI fragment of phytoene desaturase (Pds) cDNA (21), and GGPP synthase as detailed (22). Analysis of gene expression by reverse transcription-PCR was carried out as described (23).…”

Section: Transformation Of Tomato Plants and A Tumefaciensmentioning

confidence: 99%

Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

Fraser

Römer

Shipton

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

437

278

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Phytoene synthase from the bacterium Erwinia uredovora (crtB) has been overexpressed in tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary transformants (T 0) were 2-4-fold higher than the controls, whereas phytoene, lycopene, ␤-carotene, and lutein levels were increased 2.4-, 1.8-, and 2.2-fold, respectively. The biosynthetically related isoprenoids, tocopherols plastoquinone and ubiquinone, were unaffected by changes in carotenoid levels. The progeny (T 1 and T2 generations) inherited both the transgene and phenotype. Determination of enzyme activity and Western blot analysis revealed that the CRTB protein was plastid-located and catalytically active, with 5-10-fold elevations in total phytoene synthase activity. Metabolic control analysis suggests that the presence of an additional phytoene synthase reduces the regulatory effect of this step over the carotenoid pathway. The activities of other enzymes in the pathway (isopentenyl diphosphate isomerase, geranylgeranyl diphosphate synthase, and incorporation of isopentenyl diphosphate into phytoene) were not significantly altered by the presence of the bacterial phytoene synthase.carotenoids ͉ metabolic engineering

show abstract

A single polypeptide catalyzing the conversion of phytoene to zeta-carotene is transcriptionally regulated during tomato fruit ripening.

Cited by 183 publications

References 24 publications

A comprehensive review on the colorless carotenoids phytoene and phytofluene

A comprehensive review on the colorless carotenoids phytoene and phytofluene

Molecular cloning and functional expression in E. coli of a novel plant enzyme mediating ξ‐carotene desaturation

Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner

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