A single ortholog of teashirt and tiptop regulates larval pigmentation and adult appendage patterning in Bombyx mori

Zhang, Ru; Zhang, Zhongjie; Huang, Yongping; Qian, Airong; Tan, Anjiang

doi:10.1016/j.ibmb.2020.103369

Cited by 8 publications

(1 citation statement)

References 34 publications

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“…Previous studies integrating CRISPR/Cas9 components into lepidopterans are limited to the highly domesticated model species Bombyx mori (silkworm). There, using the IE1 promoter to drive Cas9 resulted in somatic editing of transheterozygotes [39][40][41] , while using the B. mori nanosP promoter resulted in both somatic mosaicism of transheterozygotes 30,[42][43][44][45][46][47][48][49][50][51][52][53] and some germline activity [54][55][56] . However, these studies provide no rates for these activities and due to their experimental design, it is often difficult to disentangle the effects of germline edits from somatic mosaicism.…”

Section: Discussionmentioning

confidence: 99%

Towards CRISPR/Cas9-based gene drive in the diamondback moth Plutella xylostella

Harvey‐Samuel

Siddiqui

et al. 2021

Preprint

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Promising to provide powerful genetic control tools, gene drives have been constructed in multiple dipterans, yeast and mice, for the purposes of population elimination or modification. However, it remains unclear whether these techniques can be applied to lepidopterans. Here, we used endogenous regulatory elements to drive Cas9 and sgRNA expression in the diamondback moth, (Plutella xylostella), and test the first split-drive system in a lepidopteran. The diamondback moth is an economically important global agriculture pest of cruciferous crops and has developed severe resistance to various insecticides, making it a prime candidate for such novel control strategy development. A very high level of somatic editing was observed in Cas9/sgRNA transheterozygotes, although no significant homing was revealed in the subsequent generation. Although heritable, Cas9-medated germline cleavage, as well as maternal and paternal Cas9 deposition was observed, rates were far lower than for somatic cleavage events, indicating robust somatic but limited germline activity of Cas9/sgRNA under the control of selected regulatory elements. Our results provide valuable experience, paving the way for future construction of gene drive-based genetic control strategies in DBM or other lepidopterans.

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