A single NLR gene confers resistance to leaf and stripe rust in wheat

Sharon, Amir; Sharma, Davinder; Avni, Raz; Gutierrez-Gonzalez, Juan; Kumar, Rakesh; Sela, Hanan; Prusty, Manas; Cohen, Arava; Molnar, Istvan; Holušová, Kateřina; Said, Mahmoud; Doležel, Jaroslav; Millet, Eitan; Khazan, Sofia; Landau, Udi; Bethke, Gerit; Ezrati, Smadar; Ronen, Moshe; Maatuk, Oxana; Eilam, Tamar; Manisterski, Jacob; Ben-Yehuda, Pnina; Anikster, Yehoshua; Matny, Oadi; Steffenson, Brian; Mascher, Martin; Brabham, Helen; Moscou, Matthew; Liang, Yong; Yu, Guotai; Wulff, Brande; Muehlbauer, Gary; Minz-Dub, Anna

doi:10.21203/rs.3.rs-3146908/v1

Cited by 3 publications

(2 citation statements)

References 106 publications

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“…A small number of genes of different classes have been shown to confer resistance to multiple pathogens in wheat or its progenitors. These include the NLRs Mla7 and Mla8 42 and the receptor-like kinase TuRLK1 43 , which confer resistance to wheat powdery mildew and wheat yellow rust, in addition to NLRs Lr548 and Yr548, which confer resistance to leaf and stripe rust, respectively 44 .…”

Section: Discussionmentioning

confidence: 99%

The wheat powdery mildew resistance gene Pm4 also confers resistance to wheat blast

O’Hara,

Steed,

Goddard

et al. 2024

Nat. Plants

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Wheat blast, caused by the fungus Magnaporthe oryzae, threatens global cereal production since its emergence in Brazil in 1985 and recently spread to Bangladesh and Zambia. Here we demonstrate that the AVR-Rmg8 effector, common in wheat-infecting isolates, is recognized by the gene Pm4, previously shown to confer resistance to specific races of Blumeria graminis f. sp. tritici, the cause of powdery mildew of wheat. We show that Pm4 alleles differ in their recognition of different AVR-Rmg8 alleles, and some confer resistance only in seedling leaves but not spikes, making it important to select for those alleles that function in both tissues. This study has identified a gene recognizing an important virulence factor present in wheat blast isolates in Bangladesh and Zambia and represents an important first step towards developing durably resistant wheat cultivars for these regions.

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Section: Discussionmentioning

confidence: 99%

The wheat powdery mildew resistance gene Pm4 also confers resistance to wheat blast

O’Hara,

Steed,

Goddard

et al. 2024

Nat. Plants

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“…sharonensis and Ae. longissima, which are closely related diploid species of the section Sitopsis (Sharon et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Fine mapping and characterization of rust resistance genes Lr53 and Yr35 introgressed from Aegilops species

Xu,

Shen,

Chen

et al. 2024

Preprint

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Wheat leaf rust and stripe rust are damaging fungal diseases of wheat worldwide. Breeding for resistance is a sustainable approach to control these two foliar diseases. In this study, we used SNP analysis, sequence comparisons and cytogenetic assays to determine that the chromosomal segment carrying Lr53 and Yr35 was originated from Ae. longissima or Ae. sharonensis or their derived species. In seedling tests, Lr53 conferred strong resistance against all five Chinese Pt races tested, and Yr35 showed effectiveness against Pst race CYR34 but susceptibility to race CYR32. Using a large population (3,892 recombinant gametes) derived from plants homozygous for the ph1b mutation obtained from the cross 98M71 × CSph1b, both Lr53 and Yr35 were successfully mapped to a 6.03 Mb telomeric region of chromosome arm 6BS in the Chinese Spring reference genome v1.1. Co-segregation between Lr53 and Yr35 was observed within this large mapping population. Within the candidate region, several nucleotide-binding leucine-rich repeat (NLR) genes and protein kinases were identified as candidate genes. Marker pku6B3127 was completely linked to both genes and accurately predicted the absence or presence of alien segment harboring Lr53 and Yr35 in 87 tetraploid and 149 hexaploid wheat genotypes tested. We developed a line with a smaller alien segment (< 6.03 Mb) to reduce any potential linkage drag and demonstrated that it conferred resistance levels similar to those of the original donor parent 98M71. The newly developed introgression line and closely linked PCR markers will accelerate the deployment of Lr53 and Yr35 in wheat breeding programs.

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Fine-mapping of LrN3B on wheat chromosome arm 3BS, one of the two complementary genes for adult-plant leaf rust resistance

Wang,

Li,

Qiu

et al. 2024

Theor Appl Genet

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A single NLR gene confers resistance to leaf and stripe rust in wheat

Cited by 3 publications

References 106 publications

The wheat powdery mildew resistance gene Pm4 also confers resistance to wheat blast

The wheat powdery mildew resistance gene Pm4 also confers resistance to wheat blast

Fine mapping and characterization of rust resistance genes Lr53 and Yr35 introgressed from Aegilops species

Fine-mapping of LrN3B on wheat chromosome arm 3BS, one of the two complementary genes for adult-plant leaf rust resistance

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