A Single Module Type I Polyketide Synthase Directs de Novo Macrolactone Biogenesis during Galbonolide Biosynthesis in Streptomyces galbus

Kim, Hyun-Ju; Karki, Suman; Kwon, Soyeon; Park, Si-Hyung; Nahm, Baek Hie; Kim, Yeon-Ki; Kwon, Hyung‐Jin

doi:10.1074/jbc.m114.602334

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…Intriguingly, the BIG-SCAPE analysis clustered regions containing rustmicin-like BGCs from the four S. violaceusniger strains we selected for comparative studies but did not add to this cluster the region 2 of AgN23 also annotated as rustmicin by antiSMASH (Table 2). Since the organisation of the five BGCs are very similar to the subcluster GalA-E of S. galbus responsible for the biosynthesis of the macrolactone ring of the compound, we propose that rustmicin biosynthesis is among core BGCs functions of the S. violaceusniger clade 60 (Figure 6). Given S. violaceusniger isolates are frequently isolated in interactions with plant roots, these data suggest that these specialised metabolites support their accommodation into the plant rhizosphere and root microbiota 36,61 .…”

Section: Resultsmentioning

confidence: 97%

“…Additional BGCs are likely involved in the regulation of the bacteria lifecycle such as spore pigment (region 30), hopene (region 31), and butyrolactone (region 36). BGCs encoded by regions 2, 18, 39 and 40 are similar to the biosynthesis pathways of the antifungal compounds rustmicin, also known as galbonolide A, mediomycin A, nigericin and niphimycins C-E respectively 42-45 . In addition, echoside (region 26), elaiophylin (region 38) and nigericin (region 39) are structural analogues of terfestatin, pteridic acid and monensin respectively, three compounds affecting plant immunity and development 46-50 .…”

Section: Resultsmentioning

confidence: 99%

“…Additional BGCs are likely involved in the regulation of the bacterium life cycle such as spore pigment (region 31), hopene (region 32), and butyrolactone (region 37). BGCs encoded in regions 2, 17, 41 and 42 are similar to the one belonging to the biosynthesis pathways of the antifungal compounds rustmicin, also known as galbonolide A, mediomycin A, nigericin and niphimycins C-E respectively [57][58][59][60].…”

Section: Agn23's Specialised Metabolism Targets Biological Functions ...mentioning

confidence: 99%

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Genomic adaptation in the CAZyome and specialised metabolism of the plant-associated Streptomyces violaceusniger clade

Gayrard

Veyssière

Adam

et al. 2021

Preprint

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Streptomycetes are Gram-positive actinobacteria largely represented in the plant root microbiota. The genetic determinants involved in the presence of Streptomyces in the rhizosphere are largely unknown and can rely on the ability to degrade plant-derived compounds such as cell-wall polysaccharides and on the production of specialised metabolites. To address whether Streptomyces strains recruited into root microbiota share genomic specificities related to these two functions, we engaged a comparative genomic analysis using a newly sequenced rhizospheric strain, Streptomyces sp. AgN23 and strains from the phylogenetically related S. violaceusniger clade. This analysis enlightens a shared prominent CAZyome potentially involved in plant polysaccharides degradation and a strong conservation of antimicrobials biosynthetic clusters (rustmicin, mediomycin, niphimycin, nigericin) as well as plant bioactive compounds (nigericin, echosides, elaiophylin). Taken together, our work supports the hypothesis that specific hydrolytic enzymes and specialised metabolites repertoires may play important roles in the development of Streptomyces strains in the rhizosphere.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Agn23's Specialised Metabolism Targets Biological Functions ...mentioning

confidence: 99%

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Genomic adaptation in the CAZyome and specialised metabolism of the plant-associated Streptomyces violaceusniger clade

Gayrard

Veyssière

Adam

et al. 2021

Preprint

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“…Even we used more homologues from streptomyces species and a non-streptomyces species for phylogenetic analysis and found they could be categorized into group III (Figure A in S1 File ). Blast search for the downstream and upstream genes of these homologues in their genomes supported that most of them might be related to the biosynthesis of secondary metabolites, though which structures remain unknown [ 33 – 36 ].…”

Section: Resultsmentioning

confidence: 99%

Functional Characterization of a Ketoreductase-Encoding Gene med-ORF12 Involved in the Formation of a Stereospecific Pyran Ring during the Biosynthesis of an Antitumor Antibiotic Medermycin

Yang

et al. 2015

PLoS ONE

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Medermycin, a polyketide antibiotic, possesses strong bioactivity against a variety of tumors through a novel mechanism and is structurally featured with a pyran ring containing two chiral centers (3S and 15R). By far the biosynthetic origin of such enantiomerical conformations still remains obscure. In the present study, we reported the functional characterization of a proposed ketoreductase Med-ORF12 encoded by medermycin biosynthetic cluster and revealed its involvement in the stereochemical control at C3 center of medermycin. Firstly, bioinformatics analysis of Med-ORF12 suggested that it belongs to a group of stereospecific ketoreductases. Next, a Med-ORF12-deficient mutant was obtained and LC/MS measurements demonstrated that medermycin production was completely abolished in this mutant. Meanwhile, it was found that two shunt products were accumulated at the absence of Med-ORF12. Finally, the reintroduction of Med-ORF12 into this mutant could restore the production of medermycin. In a conclusion, these data supported that Med-ORF12 is essential for the biosynthesis of medermycin and performs its role as a stereospecifc ketoreductase in the tailoring steps of medermycin biosynthetic pathway.

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“…PKS genes for L-155,175 synthesis are encoded in Scaffold03 and Scaffold21, but the gene cluster split into two scaffolds in the draft genome sequence. A galbonolide-synthetic type I PKS gene cluster ( 8 ) is present in Scaffold03. An NRRS gene cluster responsible for laspartomycin synthesis ( 9 ) is present in scaffold00021.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Streptomyces sp. SPMA113, a Prajinamide Producer

Komaki

Hosoyama²,

Ichikawa³

et al. 2016

Genome Announc

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A Single Module Type I Polyketide Synthase Directs de Novo Macrolactone Biogenesis during Galbonolide Biosynthesis in Streptomyces galbus

Cited by 13 publications

References 26 publications

Genomic adaptation in the CAZyome and specialised metabolism of the plant-associated Streptomyces violaceusniger clade

Genomic adaptation in the CAZyome and specialised metabolism of the plant-associated Streptomyces violaceusniger clade

Functional Characterization of a Ketoreductase-Encoding Gene med-ORF12 Involved in the Formation of a Stereospecific Pyran Ring during the Biosynthesis of an Antitumor Antibiotic Medermycin

Draft Genome Sequence of Streptomyces sp. SPMA113, a Prajinamide Producer

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