A Single Instillation of Amorphous Silica Nanoparticles Induced Inflammatory Responses and Tissue Damage until Day 28 after Exposure

Park, Eun-Jung; Roh, Jinkyu; Kim, Younghun; Choi, Kyunghee

doi:10.1248/jhs.57.60

Cited by 18 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we cannot rule out that some effects may occur following a longer exposure to SAS NMs, as suggested by liver toxicity detected in mice following a 10‐week oral exposure to 30 nm SAS [So et al, ]. In addition, induction of inflammation with increased release of cytokines in blood has also been observed following SAS administration intraperitoneally [Park and Park, ; Nemmar et al, ], intratracheally [Park et al, ] and intravenously [Cho et al, ; Nishimori et al, ]. Inflammation cell foci were also observed in liver [Cho et al, ].…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity of synthetic amorphous silica nanoparticles in rats following short‐term exposure. Part 1: Oral route

Tarantini

Huet

Jarry

et al. 2014

Environ and Mol Mutagen

View full text Add to dashboard Cite

Synthetic amorphous silica (SAS) in its nanosized form is now used in food applications although the potential risks for human health have not been evaluated. In this study, genotoxicity and oxidative DNA damage of two pyrogenic (NM-202 and 203) and two precipitated (NM-200 and -201) nanosized SAS were investigated in vivo in rats following oral exposure. Male Sprague Dawley rats were exposed to 5, 10, or 20 mg/kg b.w./day for three days by gavage. DNA strand breaks and oxidative DNA damage were investigated in seven tissues (blood, bone marrow from femur, liver, spleen, kidney, duodenum, and colon) with the alkaline and the (Fpg)-modified comet assays, respectively. Concomitantly, chromosomal damage was investigated in bone marrow and in colon with the micronucleus assay. Additionally, malondialdehyde (MDA), a lipid peroxidation marker, was measured in plasma. When required, a histopathological examination was also conducted. The results showed neither obvious DNA strand breaks nor oxidative damage with the comet assay, irrespective of the dose and the organ investigated. Similarly, no increases in chromosome damage in bone marrow or lipid peroxidation in plasma were detected. However, although the response was not dose-dependent, a weak increase in the percentage of micronucleated cells was observed in the colon of rats treated with the two pyrogenic SAS at the lowest dose (5 mg/kg b.w./day). Additional data are required to confirm this result, considering in particular, the role of agglomeration/aggregation of SAS NMs in their uptake by intestinal cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Genotoxicity of synthetic amorphous silica nanoparticles in rats following short‐term exposure. Part 1: Oral route

Tarantini

Huet

Jarry

et al. 2014

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…As a second nanoparticle model, 50 nm plain silica nanoparticles (SiO 2 ) were selected. These are generally well tolerated by cells (Shapero et al 2011;Lesniak et al 2012;Ye et al 2017) and are often considered biocompatible, although some studies suggest that also these nanoparticles can interfere with the physiological cellular behavior (Mohamed et al 2011) and others indicate that they can elicit inflammatory responses in phagocytes (Park et al 2011;Kusaka et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Time-resolved characterization of the mechanisms of toxicity induced by silica and amino-modified polystyrene on alveolar-like macrophages

et al. 2019

View full text Add to dashboard Cite

Macrophages play a major role in the removal of foreign materials, including nano-sized materials, such as nanomedicines and other nanoparticles, which they accumulate very efficiently. Because of this, it is recognized that for a safe development of nanotechnologies and nanomedicine, it is essential to investigate potential effects induced by nano-sized materials on macrophages. To this aim, in this work, a recently established model of primary murine alveolar-like macrophages was used to investigate macrophage responses to two well-known nanoparticle models: 50 nm amino-modified polystyrene, known to induce cell death via lysosomal damage and apoptosis in different cell types, and 50 nm silica nanoparticles, which are generally considered non-toxic. Then, a time-resolved study was performed to characterize in detail the response of the macrophages following exposure to the two nanoparticles. As expected, exposure to the amino-modified polystyrene led to cell death, but surprisingly no lysosomal swelling or apoptosis were detected. On the contrary, a peculiar mitochondrial membrane hyperpolarization was observed, accompanied by endoplasmic reticulum stress (ER stress), increased cellular reactive oxygen species (ROS) and changes of metabolic activity, ultimately leading to cell death. Strong toxic responses were observed also after exposure to silica, which included mitochondrial ROS production, mitochondrial depolarization and cell death by apoptosis. Overall, these results showed that exposure to the two nanoparticles led to a very different series of intracellular events, suggesting that the macrophages responded differently to the two nanoparticle models. Similar time-resolved studies are required to characterize the response of macrophages to nanoparticles, as a key parameter in nanosafety assessment.

show abstract

“…In short term inhalation studies nonsurface modified amorphous SiO 2 was able to induce slight or marked inflammation [8,9]. Pulmonary inflammation and lung tissue damage was also detected in instillation studies in mice [10]. Skin penetration of SiO 2 NP was detected in vivo and in vitro [11,12].…”

Section: Introductionmentioning

confidence: 99%

Size and Cell Type Dependent Uptake of Silica Nanoparticles

Hsiao

Gramatke

Joksimovic

et al. 2014

J Nanomed Nanotechnol

View full text Add to dashboard Cite

As silica nanoparticles (SiO 2 NP) gain increasing interest for medical applications it is important to understand their potential adverse effects for humans. Here we prepared well-defined core-shell fluorescently labelled SiO 2 NP of 15, 60 and 200 nm diameter and analyzed their cytotoxicity in THP-1 derived macrophages, A549 epithelial cells, HaCaT keratinocytes and NRK-52E kidney cells. We observed a size-dependent cytotoxicity in all cell types in serumfree conditions. HaCaT cells were least and macrophages or lung derived A549 cells were highly sensitive towards SiO 2 NP treatment. Differences in cytotoxicity could be correlated with different uptake rates. By using flow cytometry and confocal microscopy we quantified the uptake. Furthermore we used specific inhibitors for clathrin-and caveolinmediated endocytosis to elucidate the uptake mechanisms, which were found to be dependent on the NP size and the cell type. Clathrin-mediated endocytosis was involved in the uptake of SiO 2 NP of all sizes and was the major pathway for 60 nm or 200 nm SiO 2 NP. Caveolin-mediated endocytosis contributed to the uptake of 60 and 200 nm SiO 2 NP in THP-1 macrophages but only to uptake of 200 nm SiO 2 NP in A549. However, in the presence of serum all SiO 2 NP were non-toxic. The presence of serum furthermore could alter the uptake mechanism. In summary, this study demonstrates size-and cell type dependent differences in SiO 2 NP uptake and toxicity.

show abstract

A Single Instillation of Amorphous Silica Nanoparticles Induced Inflammatory Responses and Tissue Damage until Day 28 after Exposure

Cited by 18 publications

References 45 publications

Genotoxicity of synthetic amorphous silica nanoparticles in rats following short‐term exposure. Part 1: Oral route

Genotoxicity of synthetic amorphous silica nanoparticles in rats following short‐term exposure. Part 1: Oral route

Time-resolved characterization of the mechanisms of toxicity induced by silica and amino-modified polystyrene on alveolar-like macrophages

Size and Cell Type Dependent Uptake of Silica Nanoparticles

Contact Info

Product

Resources

About