2010
DOI: 10.1186/1743-422x-7-197
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A single dose of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1 influenza virus

Abstract: BackgroundHighly pathogenic avian influenza virus subtype H5N1 infects humans with a high fatality rate and has pandemic potential. Vaccination is the preferred approach for prevention of H5N1 infection. Seasonal influenza virus infection has been reported to provide heterosubtypic immunity against influenza A virus infection to some extend. In this study, we used a mouse model pre-exposed to an H1N1 influenza virus and evaluated the protective ability provided by a single dose of DNA vaccines encoding conserv… Show more

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Cited by 11 publications
(7 citation statements)
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References 40 publications
(44 reference statements)
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“…Vaccine preparation. Plasmid pCAGGSP7/M1(p7/M1) was constructed by cloning PCR products of M gene from the A/Chicken/Jiangsu/7/2002 (H9N2) influenza virus strain into the eukaryotic expression vector pCAGGSP7(p7), as described previously 41 .…”
Section: Methodsmentioning
confidence: 99%
“…Vaccine preparation. Plasmid pCAGGSP7/M1(p7/M1) was constructed by cloning PCR products of M gene from the A/Chicken/Jiangsu/7/2002 (H9N2) influenza virus strain into the eukaryotic expression vector pCAGGSP7(p7), as described previously 41 .…”
Section: Methodsmentioning
confidence: 99%
“…Thereafter, 1% chicken red blood cells were added and incubated at room temperature for 30 minutes. The HAI titer is the reciprocal of the highest serum dilution that completely inhibits hemagglutination [ 22 ].…”
Section: Methodsmentioning
confidence: 99%
“…Despite this, studies have indicated that DNA vaccines may have a clinical application in pandemic settings. Chang et al ( 108 ) demonstrated that mice which had been pre-exposed to H1N1 were significantly protected from lethal H5N1 challenge after DNA vaccination with H5N1 NP- and M1-expressing plasmids. Given the commonality of H1N1 exposure amongst the public, this suggests DNA vaccines could be rapidly deployed to protect a large susceptible population against H5N1 outbreaks.…”
Section: Prime-boost Strategiesmentioning
confidence: 99%