A single dose immunization with rabbit haemorrhagic disease virus major capsid protein produced in Saccharomyces cerevisiae induces protection.

Boga, José Antonio; Alonso, José-María; Casáis, Rosa; Parra, Francisco

doi:10.1099/0022-1317-78-9-2315

Cited by 44 publications

(32 citation statements)

References 15 publications

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“…Viruses used include VV (38), avipoxviruses (20), raccoon poxvirus (21), capripoxvirus (53), swinepox virus (58), and myxoma virus (4,27). Second, the expression of RHDV VP60 capsid protein in several heterologous systems has been shown to induce protective immunity (4,5,8,9,20,28). Remarkably, no indications of toxicity or side effects associated to the expression of VP60 have been reported.…”

Section: Discussionmentioning

confidence: 99%

“…The VP60 gene from RHDV AST/89 strain (nucleotides 5305 to 7125) was previously cloned in plasmid pMA91 (35), yielding expression vector pMAVP60 (9). In this construct, the VP60 gene is flanked by BglII restriction sites.…”

Section: Cells and Virusesmentioning

confidence: 99%

“…The recombinant VP60 obtained in all these systems has been shown to induce protection of rabbits against a lethal challenge with RHDV. In some cases the recombinant VP60 protein selfassembled to form virus-like particles which were highly immunogenic (9,28,41,56). Both virus-like particles (50) and a recombinant vaccinia virus-VP60 virus (5) have been shown to confer protection against RHDV by the oral route.…”

mentioning

confidence: 99%

“…In recent years, the RHDV capsid protein gene has been successfully expressed in several heterologous systems. To date, VP60 protein has been produced in Escherichia coli (8); Saccharomyces cerevisiae (9), recombinant virus-based systems such as baculovirus (28,32,41,56) and poxvirus (4,5,20), and plants (12). The recombinant VP60 obtained in all these systems has been shown to induce protection of rabbits against a lethal challenge with RHDV.…”

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confidence: 99%

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Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus

Bárcena¹,

Morales²,

Vázquez³

et al. 2000

J Virol

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We have developed a new strategy for immunization of wild rabbit populations against myxomatosis and rabbit hemorrhagic disease (RHD) that uses recombinant viruses based on a naturally attenuated field strain of myxoma virus (MV). The recombinant viruses expressed the RHDV major capsid protein (VP60) including a linear epitope tag from the transmissible gastroenteritis virus (TGEV) nucleoprotein. Following inoculation, the recombinant viruses induced specific antibody responses against MV, RHDV, and the TGEV tag. Immunization of wild rabbits by the subcutaneous and oral routes conferred protection against virulent RHDV and MV challenges. The recombinant viruses showed a limited horizontal transmission capacity, either by direct contact or in a flea-mediated process, promoting immunization of contact uninoculated animals.

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Section: Discussionmentioning

confidence: 99%

Section: Cells and Virusesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus

Bárcena¹,

Morales²,

Vázquez³

et al. 2000

J Virol

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“…Until now, the VP60 protein has been derived from diverse sources, such as recombinant virus based systems (Sibilia et al, 1995), which included baculoviruses (Nagesha et al, 1995), Escherichia coli (Bertagnoli et al, 1996), Saccharomyces cerevisiae (Boga et al, 1997) and pox viruses (Fischer et al, 1997), and even from plant sources (Castañón et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Clinical chemistry, haematology, immune response and histological evaluation of rabbits after immunisation and challenge with rabbit haemorrhagic disease (RHD) virus

et al. 2017

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Following their immunisation and infection with a VSHI-CN-6 viral strain, a group of 15 rabbits were examined in a study of Rabbit Haemorrhagic Disease (RHD). Serum samples were collected from the external ear vein at 0, 15, 30 and 60 days post-immunisation. The recorded platelet numbers were closer to the lower physiological limit, indicating a mild thrombocytopenia, with values ranging from 26.6 to 30.43×10 4 /mm 3 . The phagocytic index revealed significant differences (P<0.001) between the mean values obtained before vaccination (day 0) and the 3 post-vaccination measurements, confirming the increase in phagocytic capacity after immunisation. Additionally, the serum lysozyme average value equalled 9.14 mg/mL post-vaccination. The analysis of variance revealed significant statistical differences (P<0.05) between the average values obtained before vaccination (0) and the post-vaccination values, measured on day 14 and 30, respectively. The morphology of the samples collected from the main organs involved in immune protection, spleen and gastric and portal lymph nodes highlighted changes corresponding to the post-vaccination immunological response. The white pulp of the spleen appeared as a diffuse lymphoid tissue, presenting with primary and secondary lymphoid follicles. The ratio of white/red pulp was in favour of the white pulp and multiple lymphoid follicles were present, indicating their reactivation. In the medullary area of gastric and portal lymph nodes, narrow lymphoid cords, circumscribed by relatively large lymphatic sinuses, and well defined lymphocytolysis were observed. Moreover, the exudate and lymphoid follicles during activation were noted in the cortical area. Furthermore, the inflammatory processes were identified, morphologically manifested by the thickening of connective tissue in the lymph node capsule, dilacerations of the connective fibres and the presence of light acidophilic serous exudate with rare inflammatory cells (serous lymphoreticulitis).

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Versatile RHDV virus‐like particles: Incorporation of antigens by genetic modification and chemical conjugation

Peacey

Wilson

Baird

et al. 2007

Biotech & Bioengineering

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Virus-like particles have proved to be excellent molecular scaffolds, yet the individual characteristics and immune responses generated against each VLP requires the development of a wide range of capsids for use as vaccines, molecular delivery vessels, and nanoscale templates. Here we describe the development of Rabbit haemorrhagic disease virus (RHDV)-like particles as a rapidly versatile molecular workbench, overcoming limitations imposed by established genetic antigen incorporation procedures with chimeric VLP. Production of the RHDV capsid protein in a baculovirus system led to the self-assembly of VLP which were recovered at over 99% purity and manipulated both genetically and chemically. Fusion of small peptide sequences to RHDV VLP was well tolerated, forming chimeric capsids that enhanced the presentation of foreign peptide to hybridoma T helper cells 700-fold. Rapid and simple conjugation techniques employing the hetero-bifunctional chemical linker sulfo-SMCC enabled both small peptides and whole proteins to be conjugated to the surface of RHDV VLP, overcoming limitations imposed on VLP formation and yield experienced with chimeric VLP. Administration of VLP/ovalbumin conjugate provoked high titre ovalbumin-specific antibody in mice, demonstrating the immune stimulatory properties of the capsid were conferred to conjugated foreign antigen. VLP facilitated delivery of conjugated antigen to dendritic cells, eliciting proliferative responses in naïve TCR transgenic T helper cells that were at least 10-fold greater than ovalbumin antigen delivered alone.

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A single dose immunization with rabbit haemorrhagic disease virus major capsid protein produced in Saccharomyces cerevisiae induces protection.

Cited by 44 publications

References 15 publications

Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus

Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus

Clinical chemistry, haematology, immune response and histological evaluation of rabbits after immunisation and challenge with rabbit haemorrhagic disease (RHD) virus

Versatile RHDV virus‐like particles: Incorporation of antigens by genetic modification and chemical conjugation

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