A single copy of apolipoprotein B-48 is present on the human chylomicron remnant

Phillips, Martin; Pullinger, Clive R.; Kroes, Ivo; Kroes, J.G.; Hardman, David A.; Chen, G; Curtiss, Linda K.; Gutiérrez, Mónica; Kane, John P.; Schumaker, Verne N.

doi:10.1016/s0022-2275(20)37199-6

Cited by 92 publications

(19 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ApoB48 is associated with CM particles and plays a critical role in the formation and secretion of CM particles (Lo et al 2008). It has been demonstrated that one molecule of apoB-48 is associated with one CM particle (Phillips et al 1997). Accordingly, the mass of lymphatic apoB48 secretion was used as a surrogate measure to evaluate the relative number of CM particles produced by the gut (Hayashi et al 1990a).…”

Section: Figure 7 Comparison Of the Total Recovery Of A [ 3 H]-tag (A...mentioning

confidence: 99%

Sexual dimorphism in intestinal absorption and lymphatic transport of dietary lipids

Liu

Shen

Yang

et al. 2021

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

Section: Figure 7 Comparison Of the Total Recovery Of A [ 3 H]-tag (A...mentioning

confidence: 99%

Sexual dimorphism in intestinal absorption and lymphatic transport of dietary lipids

Liu

Shen

Yang

et al. 2021

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…Apolipoprotein (apo) B -48 is the lower molecular weight form of apoB which, in humans, is produced only by the intestine (12). One molecule of apoB-48 is present per TG-rich lipoprotein (13). It remains associated with intestinal lipoproteins throughout the lipolytic cascade and is not exchanged with other lipoproteins (12,14).…”

mentioning

confidence: 99%

Apolipoprotein B-48 and retinyl palmitate are not equivalent markers of postprandial intestinal lipoproteins

Lemieux

Fontani

Uffelman

et al. 1998

Journal of Lipid Research

View full text Add to dashboard Cite

This study compared retinyl palmitate and apolipoprotein (apo) B-48 as markers of postprandial triglyceride-rich lipoproteins. Nine non-diabetic men received an oral vitamin A-containing fat load. We measured retinyl palmitate, apoB-48, apoB-100, and triglyceride levels in S f Ͼ 400, S f 60-400 and S f 20-60 lipoproteins. The peak retinyl palmitate concentration was delayed compared to the peak apoB-48 concentration in each fraction. The discrepancy between retinyl palmitate and apoB-48 was further investigated in another study of 12 men. In that study, a fat load was given and 5 h later, lipolysis was stimulated in vivo with heparin (60 U/kg, i.v.) and the same parameters were followed. Thirty minutes after heparin, triglyceride levels decreased significantly in the three triglyceride-rich lipoprotein fractions (S f Ͼ 400, S f 60-400 and S f 20-60). ApoB-48 levels also fell significantly in the three triglyceride-rich lipoprotein fractions. In contrast, retinyl palmitate concentrations did not change significantly in S f Ͼ 400 and S f 60-400 fractions and increased significantly in the S f 20-60 fraction. Our results indicate that retinyl palmitate and apolipoprotein B-48 do not mark the same properties of postprandial intestinal lipoproteins. The metabolic pattern of apolipoprotein B-48 parallels that of triglyceride. One possible explanation for these obser vations is that the apoB-48-containing triglyceride-rich lipoproteins are metabolically heterogeneous and that older particles, those in circulation for a longer period of time, may be clear ed more rapidly than newer ones.-

show abstract

“…ApoB-48, serving as a lipid acceptor and structural protein of CMs, plays a critical role in the formation and secretion of CM particles [ 25 ]. It has been clearly demonstrated that one molecule of apoB-48 is associated with one CM particle [ 26 ]. Accordingly, the mass of lymphatic apoB-48 secretion has been used as a surrogate measure to evaluate the relative number of CM particles produced by the gut [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Sequential Lipid Meals on Lymphatic Lipid Absorption and Transport in Rats

Zhu

Yang

Shen

et al. 2022

Genes

View full text Add to dashboard Cite

The sequential meal pattern has recently received more attention because it reflects a phycological diet style for human beings. The present study investigated the effects of the second lipid meal on lymphatic lipid absorption and transport in adult rats following a previous lipid meal. Using the well-established lymph fistula model, we found that the second lipid meal significantly increased the lymphatic output of triglycerides, cholesterol, phospholipids, and non-esterified fatty acids compared with a single lipid meal. Besides that, the time reaching the peak of each lipid output was significantly faster compared with the first lipid meal. Additionally, the second lipid meal significantly increased the lymphatic output of apolipoprotein A-IV (ApoA-IV), but not apolipoprotein B-48 (ApoB-48) or apolipoprotein A-I (ApoA-I). Interestingly, the triglyceride/apoB-48 ratio was significantly increased after the second lipid meal, indicating the increased chylomicron size in the lymph. Finally, the second lipid meal increased the lymphatic output of rat mucosal mast cell protease II (RMCPII). No change was found in the expression of genes related to the permeability of lymphatic lacteals, including vascular endothelial growth factor-A (Vegfa), vascular endothelial growth factor receptor 1 (Flt1), and Neuropilin1 (Nrp1). Collectively, the second lipid meal led to the rapid appearance of bigger-sized chylomicrons in the lymph. It also increased the lymphatic output of various lipids and apoA-IV, and mucosal mast cell activity in the intestine.

show abstract

A single copy of apolipoprotein B-48 is present on the human chylomicron remnant

Cited by 92 publications

References 21 publications

Sexual dimorphism in intestinal absorption and lymphatic transport of dietary lipids

Sexual dimorphism in intestinal absorption and lymphatic transport of dietary lipids

Apolipoprotein B-48 and retinyl palmitate are not equivalent markers of postprandial intestinal lipoproteins

Impact of Sequential Lipid Meals on Lymphatic Lipid Absorption and Transport in Rats

Contact Info

Product

Resources

About