A Single-Column Procedure on Bond Elut Certify for Systematic Toxicological Analysis of Drugs in Plasma and Urine

Chen, X H; Wijsbeek, J.; Franke, Jp; Zeeuw, Rokus A. de

doi:10.1520/jfs13211j

Cited by 66 publications

(18 citation statements)

References 11 publications

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“…There are numerous publications on SPE for relatively clean biological samples such as plasma, serum or urine [3][4][5][6][7][8][9][10][11][12]. Until now, only a few publications have described SPE methods for whole blood [13][14][15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Determination of several psychiatric drugs in whole blood using capillary gas–liquid chromatography with nitrogen phosphorus detection: comparison of two solid phase extraction procedures

Torre

Martínez

Almarza

2005

Forensic Science International

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Section: Introductionmentioning

confidence: 99%

Determination of several psychiatric drugs in whole blood using capillary gas–liquid chromatography with nitrogen phosphorus detection: comparison of two solid phase extraction procedures

Torre

Martínez

Almarza

2005

Forensic Science International

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“…Recently, Suzuki et al [2] reported a rapid extraction method for some barbiturates in plasma, whole blood and urine using a Sep-Pak C18 cartridge. Chen et al [3] developed a systematic extraction method for drugs in plasma and urine using a Bond Elut Certify column.…”

Section: Discussionmentioning

confidence: 99%

“…When a Bond Elut Certify column was used for extraction, the procedure reported by Chen et al [3] was used.…”

Section: Methodsmentioning

confidence: 99%

A rapid method for detecting barbiturates in serum using EI-SIM

et al. 1994

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A simple and rapid method for analysis of barbiturates in serum has been developed. In order to extract and clean barbiturates in serum, a separation column packed with Extrelut and Florisil was used, and the eluate was directly analyzed by means of electron impact selected ion monitoring (EI-SIM). Selected ions used were base peak ions of 10 barbituartes, and the internal standard used was allobarbital or secobarbital. The calibration curves were linear over the range 0.5-5 ng. Extraction of replicate serum samples containing 20 micrograms/1.5 ml and 5 micrograms/1.5 ml resulted in a recovery of 87.2-105.2% and 81.6-104.6%, respectively, with the exception of phenobarbital, which was 151.9% and 172.1%, respectively. Secobarbital was also analyzed in the serum of 13 patients who had been given secobarbital intravenously. In 3 out of 10 cases, secobarbital levels greater than 1 micrograms/ml were detected more than 72 h after administration. This method seems to have possibilities for clinical use.

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“…Firstly, the initial dilution was increased twofold (method B) to modify the contact time between the analyte and the extraction sorbent. Dilution of the sample is also a common procedure for reducing the protein binding problem [18][19][20]. On MP3-plus, with this method, the retention ratio was 8 % higher than with method A (retention ratio = 61.8 %, RSD = 5.2 %; n = 6).…”

Section: Preparation Of the Serummentioning

confidence: 94%

Evaluation of procedures for solid-phase extraction of [125I]-methodone from serum on to discs and cartridges

Rudaz¹,

Haerdi²,

Veuthey³

1997

Chromatographia

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Biological samples must be purified before chromatography to eliminate interfering compounds. Discs have recently been developed to enhance the performance of solid-phase extraction (SPE) and reduce solvent consumption. This type of support is available in all the bonded-phases typically found in conventional packed SPE devices. The process for solid-phase extraction of [125I]-methadone from water and serum (i.e. retention and losses of [125I]-methadone during sample loading, washing, elution, etc.) has been studied to determine the behaviour of this compound on several extracting supports. For serum solutions we observed significant matrix effects on discs because of their low capacity. This resulted in loss of [125I]-methadone during the loading step because the contact time between the analyte and the functional groups of the support was too short. Although mixed-mode discs, which have both hydrophobic and cation-exchange extraction mechanisms, do not perform as well as conventional cartridges, they can, nevertheless, be used before liquid chromatographic analysis because of their several mechanical and economical advantages in comparison with cartridges

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A Single-Column Procedure on Bond Elut Certify for Systematic Toxicological Analysis of Drugs in Plasma and Urine

Cited by 66 publications

References 11 publications

Determination of several psychiatric drugs in whole blood using capillary gas–liquid chromatography with nitrogen phosphorus detection: comparison of two solid phase extraction procedures

Determination of several psychiatric drugs in whole blood using capillary gas–liquid chromatography with nitrogen phosphorus detection: comparison of two solid phase extraction procedures

A rapid method for detecting barbiturates in serum using EI-SIM

Evaluation of procedures for solid-phase extraction of [125I]-methodone from serum on to discs and cartridges

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