2023
DOI: 10.1002/jcsm.13292
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A single‐cell atlas of bovine skeletal muscle reveals mechanisms regulating intramuscular adipogenesis and fibrogenesis

Abstract: BackgroundIntramuscular fat (IMF) and intramuscular connective tissue (IMC) are often seen in human myopathies and are central to beef quality. The mechanisms regulating their accumulation remain poorly understood. Here, we explored the possibility of using beef cattle as a novel model for mechanistic studies of intramuscular adipogenesis and fibrogenesis.MethodsSkeletal muscle single‐cell RNAseq was performed on three cattle breeds, including Wagyu (high IMF), Brahman (abundant IMC but scarce IMF), and Wagyu/… Show more

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Cited by 10 publications
(7 citation statements)
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“…Furthermore, genes linked to the immune system such as AZU1 ( Xu et al, 2018 ; Verardo et al, 2021 ) and ELANE ( Cassatella et al, 2019 ; Verardo et al, 2021 ) were also identified. The CFD gene was also previously associated with fat accumulation ( Wang et al, 2023 ) and overlapped with the region cited above.…”
Section: Discussionmentioning
confidence: 72%
“…Furthermore, genes linked to the immune system such as AZU1 ( Xu et al, 2018 ; Verardo et al, 2021 ) and ELANE ( Cassatella et al, 2019 ; Verardo et al, 2021 ) were also identified. The CFD gene was also previously associated with fat accumulation ( Wang et al, 2023 ) and overlapped with the region cited above.…”
Section: Discussionmentioning
confidence: 72%
“…In beef cattle, there is a positive correlation between the content of intramuscular fat and capillary abundance. 2 , 3 Our 3D culture model facilitates the study of developmental interactions between blood vessels and adipocytes. During the angiogenic phase, blood vessels form, and preadipocytes are situated around these vessels.…”
Section: Before You Beginmentioning
confidence: 99%
“…Other agriculturally important traits in ruminants such as growth and fat deposition were investigated in cattle by performing scRNA-seq and scATAC-seq in skeletal muscle [14,15], scRNA-seq of cultured bovine satellite cells [22,23], and snRNA-seq of adipose tissue [7], which uncovered previously unrealized cellular heterogeneity. Both scRNA-seq and scATAC-seq were employed in the skeletal muscle of cattle at fetal, postnatal, and adult stages of development [15].…”
Section: Cellular Heterogeneity Within Tissues Important For Food And...mentioning
confidence: 99%
“…Furthermore, the application of single-cell genomics in livestock research has the potential to accelerate the pace of genetic improvement by facilitating the identification of cell-type-specific gene expression, along with gene regulatory elements and networks associated with various traits important to livestock production . Adipose Nuclei RNA GEO GSE211707 [7] Embryo (8-and 16-cell stage) Cell RNA GEO GSE99210 [8] Embryo (peri-implantation) Cell RNA GEO GSE234335 [9] Embryo (SCNT) Cell RNA SRA PRJNA727165 [10] Embryo (trophectoderm) Cell RNA GEO GSE200216 [11] Fetal Gonads Cell RNA GEO GSE162952 [12] Milk Somatic Cells Cell RNA ENA PRJEB73560 [13] Muscle Cell RNA GEO GSE205347 [14] Muscle Cell RNA, ATAC GSA CRA006626 [15] PBMC * Cell RNA, ATAC GEO GSE225962 [16] PBMC * Cell RNA, ATAC GEO GSE166473 [17] Placenta (developing) Cell RNA GEO GSE234524 [18] Placenta (mature) Nuclei RNA GEO GSE214407 [19] Primary Mammary Epithelial Cells Cell RNA FAANG PRJEB41576 [20] Ruminal epithelial cells Cell RNA GEO GSE166473 [21] Satellite cells Cell RNA GEO GSE184128 [22] Satellite cells Cell RNA GEO GSE211428 [23] Despite the potential benefits, the application of sc/snRNA-seq and sc/snATAC-seq in ruminant livestock species presents challenges. Standardized computational analysis pipelines that are species-agnostic are essential, and the discovery and annotation of cell populations can be difficult in non-model organisms and for tissues such as the rumen, which are unique to these species [44][45][46].…”
Section: Introductionmentioning
confidence: 99%