A single bout of moderate-intensity exercise increases vascular NO bioavailability and attenuates adrenergic receptor-dependent and -independent vasoconstrictor response in rat aorta

Bechara, Luiz R. G.; Tanaka, Leonardo Yuji; Santos, Adriana Marques dos; Jordão, Camila Paixão; Sousa, Luise Gustavo Oliveira de; Bartholomeu, Teresa; Ramires, P. R.

doi:10.1540/jsmr.44.101

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…Bechara et al ( 7 ) showed that improvements in the vasodilator response occurred immediately after a single exercise session performed on a treadmill at 60% of maximal exercise capacity, directly related to an increase in the bioavailability of endothelial nitric oxide and attenuation of the maximal contractile response ( 8 , 9 ). This post-exercise response model is relevant to the development of post-exercise hypotension in humans, in which both hypertensive and normotensive subjects experience a reduction in blood pressure during the first minute after a single exercise session ( 10 - 12 ), which may continue for hours after the exercise session ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Intensity of swimming exercise influences aortic reactivity in rats

Brito

Silva

Souza

et al. 2015

Braz J Med Biol Res

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Exercise is known to cause a vasodilatory response; however, the correlation between the vasorelaxant response and different training intensities has not been investigated. Therefore, this study evaluated the vascular reactivity and lipid peroxidation after different intensities of swimming exercise in rats. Male Wistar rats (aged 8 weeks; 250-300 g) underwent forced swimming for 1 h whilst tied to loads of 3, 4, 5, 6, and 8% of their body weight, respectively (groups G3, G4, G5, G6 and G8, respectively; n=5 each). Immediately after the test, the aorta was removed and suspended in an organ bath. Cumulative relaxation in response to acetylcholine (10−12-10−4 M) and contraction in response to phenylephrine (10−12-10−5 M) were measured. Oxidative stress was estimated by determining malondialdehyde concentration. The percentages of aorta relaxation were significantly higher in G3 (7.9±0.20), G4 (7.8±0.29), and G5 (7.9±0.21), compared to the control group (7.2±0.04), while relaxation in the G6 (7.4±0.25) and G8 (7.0±0.06) groups was similar to the control group. In contrast, the percentage of contraction was significantly higher in G6 (8.8 ±0.1) and G8 (9.7±0.29) compared to the control (7.1±0.1), G3 (7.3±0.2), G4 (7.2±0.1) and G5 (7.2±0.2%) groups. Lipid peroxidation levels in the aorta were similar to control levels in G3, G4 and G5, but higher in G6 and G8, and significantly higher in G8 (one-way ANOVA). These results indicate a reduction in vasorelaxing activity and an increase in contractile activity in rat aortas after high-intensity exercise, followed by an increase in lipid peroxidation.

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Section: Introductionmentioning

confidence: 99%

Intensity of swimming exercise influences aortic reactivity in rats

Brito

Silva

Souza

et al. 2015

Braz J Med Biol Res

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“…However, in the current research, participants were obliged to continue moderate intensity training until they get exhausted. The average duration of running with moderate intensity in the current research was approximately 5.15 minutes (21).…”

Section: Discussionmentioning

confidence: 73%

“…More recently, it has been shown that the activity and the expression of the eNOS enzyme is increased by acute exposure of both endothelial cells and mice aorta exposed to shear stress and high levels of adrenergic agonists. In addition, this training was also able to increase endothelial secretion of calcium and release of nitric oxide, dependent on the calcium (21).…”

Section: Discussionmentioning

confidence: 90%

The Effect of Acute and Chronic Moderate and High Intensity Aerobic Exercise on Serum Nitric Oxide in Untrained Overweight Males

Nikbakht

Nasab

Shakerian

2017

Jentashapir J Helath Res

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“…Vascular homogenates were prepared using liquid N 2 , centrifuged at 5,000 rpm for 5 min, and 10-μL aliquots of the supernatant were injected into a Sievers chemiluminescence analyzer (model 280; Sievers Instruments, Inc., Boulder, Colorado, USA) using VCl3 and HCl (at 95°C) as reductants as previously described 30,31. The results were normalized to the protein concentration.…”

Section: Methodsmentioning

confidence: 99%

Aerobic Swim Training Restores Aortic Endothelial Function by Decreasing Superoxide Levels in Spontaneously Hypertensive Rats

Jordão

Fernandes

Tanaka

et al. 2017

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OBJECTIVE:We aimed to determine whether aerobic training decreases superoxide levels, increases nitric oxide levels, and improves endothelium-dependent vasodilation in the aortas of spontaneously hypertensive rats.METHODS:Spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) were distributed into 2 groups: sedentary (SHRsd and WKYsd, n=10 each) and swimming-trained (SHRtr, n=10 and WKYtr, n=10, respectively). The trained group participated in training sessions 5 days/week for 1 h/day with an additional work load of 4% of the animal’s body weight. After a 10-week sedentary or aerobic training period, the rats were euthanized. The thoracic aortas were removed to evaluate the vasodilator response to acetylcholine (10-10 to 10-4 M) with or without preincubation with L-NG-nitro-L-arginine methyl ester hydrochloride (L-NAME; 10-4 M) in vitro. The aortic tissue was also used to assess the levels of the endothelial nitric oxide synthase and nicotinamide adenine dinucleotide oxidase subunit isoforms 1 and 4 proteins, as well as the superoxide and nitrite contents. Blood pressure was measured using a computerized tail-cuff system.RESULTS:Aerobic training significantly increased the acetylcholine-induced maximum vasodilation observed in the SHRtr group compared with the SHRsd group (85.9±4.3 vs. 71.6±5.2%). Additionally, in the SHRtr group, superoxide levels were significantly decreased, nitric oxide bioavailability was improved, and the levels of the nicotinamide adenine dinucleotide oxidase subunit isoform 4 protein were decreased compared to the SHRsd group. Moreover, after training, the blood pressure of the SHRtr group decreased compared to the SHRsd group. Exercise training had no effect on the blood pressure of the WKYtr group.CONCLUSIONS:In SHR, aerobic swim training decreased vascular superoxide generation by nicotinamide adenine dinucleotide oxidase subunit isoform 4 and increased nitric oxide bioavailability, thereby improving endothelial function.

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A single bout of moderate-intensity exercise increases vascular NO bioavailability and attenuates adrenergic receptor-dependent and -independent vasoconstrictor response in rat aorta

Cited by 10 publications

References 35 publications

Intensity of swimming exercise influences aortic reactivity in rats

Intensity of swimming exercise influences aortic reactivity in rats

The Effect of Acute and Chronic Moderate and High Intensity Aerobic Exercise on Serum Nitric Oxide in Untrained Overweight Males

Aerobic Swim Training Restores Aortic Endothelial Function by Decreasing Superoxide Levels in Spontaneously Hypertensive Rats

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