A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS

Lu, Chao; Cheng, Kai; Li, Jiaomeng; Sun, Xiaochu; Figeys, Daniel; Jiang, Jifa; Yang, Yue; Liu, Jia

doi:10.1016/j.aca.2021.339016

Cited by 5 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SIL is a commonly used strategy in LC-MS-based metabolomic studies. Previously, we have established a SIL-LC-MS approach and realized the simultaneous identification and quantification of sulfhydryl-containing metabolites . However, there have been no publications of SIL strategies used in MALDI MSI studies to date.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we have established a SIL-LC-MS approach and realized the simultaneous identification and quantification of sulfhydryl-containing metabolites. 6 However, there have been no publications of SIL strategies used in MALDI MSI studies to date. In the current study, we combined the SIL concept with MALDI probe for the first time.…”

Section: Mai-mada Obviously Enhances the Number Of Thiols Detected In...mentioning

confidence: 99%

“…The database establishment was based on our previously published method with some modifications. 6 Briefly, a mixed solution of commercially available thiol working solution and liver tissue homogenates were derivatized with a pair D 0 /D 3 -MaI-MADA to serve as reference samples. The…”

Section: Tissue Section Preparation and Hematoxylin And Eosin (Hande)...mentioning

confidence: 99%

“…It is well-known that the exact localization and relative abundance of thiols in tissues are essential to reveal their specific functions in the occurrence and progression of diseases. The conventional analytical procedure for detecting biological metabolites in tissues has irreversible damage to the structural integrity of tissue samples, which thus could not provide specific content information on thiols in certain locations anymore . Over the past few decades, in situ imaging, a nonstructural destroyed analysis technology, has been applied to make up for this shortcoming.…”

Section: Introductionmentioning

confidence: 99%

“…The conventional analytical procedure for detecting biological metabolites in tissues has irreversible damage to the structural integrity of tissue samples, which thus could not provide specific content information on thiols in certain locations anymore. 6 Over the past few decades, in situ imaging, a nonstructural destroyed analysis technology, has been applied to make up for this shortcoming. Among these techniques, fluorescence imaging (FI) and mass spectrometry imaging (MSI) are two commonly used methods to analyze thiols.…”

Section: ■ Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Quaternized Acridine Maleimide MALDI Probe Enables Mass Spectrometry Imaging of Thiols

Yuan

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Thiols are essential metabolites associated with redox imbalances and metabolic disorders in diseases. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) facilitates imaging of metabolites in tissue, but imaging of thiols remains challenging. Here we developed a method to visualize thiols using a stable isotope-labeled (SIL) MALDI probe, a mixture of unlabeled and deuterium-labeled reagents that provided adduct signals at [M] + and [M + 3] + , to identify endogenous thiols in tissue. A series of MALDI probe candidates were rationally designed, and the structure−effect relationships were determined. First, the reactivity of different warheads toward the thiol group was evaluated, and maleimide was the best for in situ derivatization. Second, an acridine fragment showed the best improvement in MS responses. Third, a permanent charge was introduced for detection improvement in the positive mode. Finally, the hydrogens of methyl group were replaced by deuterium atoms, obtaining the novel SIL MALDI probe and thus facilitating significantly the annotation of thiols. The finally obtained D 0 /D 3 -9-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)-10-methylacridin-10-ium iodide (D 0 /D 3 -MaI-MADA) enabled direct MSI of thiols in the fine structures of human liver tumors without a reduction procedure. Our work built a SIL MALDI probe for the first time and provided a strategy for the rational design of MALDI probes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Mai-mada Obviously Enhances the Number Of Thiols Detected In...mentioning

confidence: 99%

Section: Tissue Section Preparation and Hematoxylin And Eosin (Hande)...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quaternized Acridine Maleimide MALDI Probe Enables Mass Spectrometry Imaging of Thiols

Yuan

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

show abstract

Effects of quinoa flour (Chenopodium Quinoa Willd) substitution on wheat flour characteristics

Gong³

et al. 2023

Current Research in Food Science

View full text Add to dashboard Cite

Determination of chlordimeform and its metabolite residue in milk by gas chromatography–tandem mass spectrometry

Wei,

Huang,

Chen

et al. 2024

Food Research International

View full text Add to dashboard Cite

A simultaneous identification and quantification strategy for determination of sulfhydryl-containing metabolites in normal- and high-fat diet hamsters using stable isotope labeling combined with LC-MS

Cited by 5 publications

References 46 publications

Quaternized Acridine Maleimide MALDI Probe Enables Mass Spectrometry Imaging of Thiols

Quaternized Acridine Maleimide MALDI Probe Enables Mass Spectrometry Imaging of Thiols

Effects of quinoa flour (Chenopodium Quinoa Willd) substitution on wheat flour characteristics

Determination of chlordimeform and its metabolite residue in milk by gas chromatography–tandem mass spectrometry

Contact Info

Product

Resources

About