A simultaneous extraction/derivatization strategy coupled with liquid chromatography–tandem mass spectrometry for the determination of free catecholamines in biological fluids

Chen, Di; Zhang, Jingxian; Cui, Weiqi; Zhang, Junwei; Wu, Deqiao; Yu, Xinrui; Luo, Yang; Jiang, Xingyi; Zhu, Fang; Hussain, Dilshad; Xu, Xia

doi:10.1016/j.chroma.2021.462474

Cited by 33 publications

(6 citation statements)

References 30 publications

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“…Индивидуальные исходные растворы (stock solution, 1 мг/мл) моноаминовых НТ готовили на метаноле (J.T. Backer, Польша) путём растворения в мерных колбах объёмом 50 мл 50 мг субстанции каждого аналита в пересчёте на основание [15]. Использовали аналитические стандарты Sigma Aldrich: серотонина гидрохлорид (CAS: 153-98-0), норэпинефрина гидрохлорид (CAS: 55-27-6), эпинефрина гидрохлорид (CAS: 329-63-5), дофамина гидрохлорид (CAS: 62-31-7), гистамин (CAS: 51-45-6), в качестве внутреннего стандарта (IS) применяли сальбутамол (CAS: 18559-94-9).…”

Section: материалы и методы / Materials and Methodsunclassified

Quantitative determination of monoamine neurotransmitters in rat brain homogenates using HPLC-MS/MS

Popov

Gavrilenko

Balabanyan

et al. 2023

Fk&Fd

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Relevance. Evaluation of the effect of drugs on neurotransmitter processes is an important component of pharmacodynamic studies. The quantitative determination of monoamine neurotransmitters in the brain structures of laboratory animals is an urgent task of pharmacology and physiology.Purpose of the study. Development of a method for the quantitative determination of serotonin, dopamine, norepinephrine, histamine and epinephrine in rat brain homogenates using HPLC-MS/MS.Methods. The isolation of neurotransmitters from the brain of rats was carried out by homogenizing the biomaterial with acetonitrile and hydrochloric acid. The extraction was purified by liquid-liquid extraction with chloroform and isopropanol. Monoamines were detected using an AB Sciex QTrap 3200MD mass spectrometer, chromatography was performed using an Agilent Technologies 1260 Infinity II HPLC. Methanol and deionized water were used as eluent.Results. Sample preparation consisted of centrifugation of the resulting homogenate, drying of the supernatant in a stream of nitrogen, dissolution of the precipitate in the mobile phase, and purification of the solution using a mixture of chloroform and isopropanol. An Agilent InfinityLab Poroshell 120 EC-C18 4.6×100 mm, 2.7 μm analytical column was used to separate monoamine neurotransmitters. The total time of the chromatographic analysis was 12 minutes, the retention time of norepinephrine, epinephrine, dopamine, serotonin, histamine was 2.8; 3.2; 5.4; 7.9; and 2.2 minutes, respectively. The analytical range of the technique was 25.0–5000.0 ng/g for epinephrine, histamine, and dopamine; 5.0–5000.0 ng/g for serotonin and 50.0–5000.0 for norepinephrine. To test the technique, we analyzed monoamine neurotransmitters in the striatum of intact Wistar rats.Conclusion. The developed bioanalytical HPLC-MS/MS method for the quantitative determination of monoamine neurotransmitters in the rat brain fully complies with the validation requirements. The metrological characteristics of the technique make it possible to estimate the content of norepinephrine, epinephrine, dopamine, serotonin, and histamine in the brain structures of rats with high accuracy.

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Section: материалы и методы / Materials and Methodsunclassified

Quantitative determination of monoamine neurotransmitters in rat brain homogenates using HPLC-MS/MS

Popov

Gavrilenko

Balabanyan

et al. 2023

Fk&Fd

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“…Among them, the electrochemical sensor has the advantages of good portability, strong operability, short response time, low cost, low detection limits, and high sensitivity, and has been widely applied to detect and continuously monitor various metabolites in body biofluids. 24,25 Therefore, it was considered to construct an electrochemical sensor for the simultaneous detection of three substances. It has been shown that the electrochemical REDOX peak of a substance can be used for its specific identification when an electrochemical sensor is used to detect the substance.…”

Section: Introductionmentioning

confidence: 99%

An amorphous zinc/copper double transition metal hydroxides for electrochemical simultaneous detection of dopamine, serotonin, and melatonin

et al. 2023

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“…HPLC methods are more preferable than GC because biogenic amines are low volatile and have the functional groups to be derivatized for spectrophotometric detections. The application of derivatization in chromatography is the subject of many studies [ 21 , 22 , 23 ]. This process consists of converting the analytes into appropriate derivatives with properties enabling their chromatographic separation.…”

Section: Introductionmentioning

confidence: 99%

Development of a High Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD) Method for Determination of Biogenic Amines in Ripened Cheeses

et al. 2022

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Biogenic amines (BAs) are organic, basic nitrogenous compounds formed during the decarboxylation of amino acids. A method for the determination of eight biogenic amines (tryptamine, 2-phenyletylamine, putrescine, cadaverine, histamine, tyramine, spermidine, spermine) in ripened cheeses was developed and validated. Cheese samples with the addition of internal standards were extracted with 0.2 M perchloric acid and pre-column derivatized with dansyl chloride at 60 °C for 15 min, purified with toluene and dried under a stream of nitrogen. The samples were analyzed using high performance liquid chromatography with diode array detector (HPLC-DAD). The method was validated with the BAs at three concentration levels: 50, 100, and 200 mg/kg, respectively. The obtained values of correlation coefficient (R2) ranged at 0.9997–0.9998 for all of compounds. The limits of detection (LOD) and quantification (LOQ) were in ranges 1.53–1.88 and 5.13–6.28 mg/kg, respectively. The recovery for all of biogenic amines ranged from 70 to 120% and the precision (RSDr) value were <20%. The validated method was applied to analysis of 35 real ripened cheese samples purchased in Poland.

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A simultaneous extraction/derivatization strategy coupled with liquid chromatography–tandem mass spectrometry for the determination of free catecholamines in biological fluids

Cited by 33 publications

References 30 publications

Quantitative determination of monoamine neurotransmitters in rat brain homogenates using HPLC-MS/MS

Quantitative determination of monoamine neurotransmitters in rat brain homogenates using HPLC-MS/MS

An amorphous zinc/copper double transition metal hydroxides for electrochemical simultaneous detection of dopamine, serotonin, and melatonin

Development of a High Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD) Method for Determination of Biogenic Amines in Ripened Cheeses

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