A Simultaneous Determination of Daidzin and Puerarin and Determination of Daidzein in Oriental Pharmaceutical Decoctions Containing Puerariae Radix by Ion-Pair High-Performance Liquid Chromatography

Yasuda, Takaaki; Momma, Noriko; Ohsawa, Keisuke

doi:10.1248/yakushi1947.113.12_881

Cited by 8 publications

(1 citation statement)

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“…At present, many analytical methods have been used to analyze puerarin, including reversed-phase high-performance liquid chromatography (RP-HPLC) [6][7][8], capillary electrophoresis (CE) [9], and ion chromatography (IC) [10]. Hyphenated techniques, especially LC-tandem MS [11][12][13] has been increasingly used to enhance the sensitivity and provide a wealthy information for on-line compositional and structural analysis.…”

Section: Introductionmentioning

confidence: 99%

Quantification of puerarin in plasma by on-line solid-phase extraction column switching liquid chromatography–tandem mass spectrometry and its applications to a pharmacokinetic study

Wang

Dai

et al. 2008

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Quantification of puerarin in plasma by on-line solid-phase extraction column switching liquid chromatography–tandem mass spectrometry and its applications to a pharmacokinetic study

Wang

Dai

et al. 2008

Journal of Chromatography B

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Determination of 12 pueraria components by high‐performance liquid chromatography‐mass spectrometry

Lin¹,

Sheu

2005

J of Separation Science

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Puerariae radix, a commonly used Chinese herb drug derived from the dried root of legume plant, contains a series of isoflavones as its chief pharmacologically active constituents. Using 12 pueraria components as markers, an LC-UV-MS method requiring less than 60 min, was developed for estimating the quality of pueraria samples within 60 min. Extracts were analyzed using a Cosmosil 5C18-MS column, by gradient elution with an aqueous solution of acetic acid and methanol-ACN at a flow-rate of 1.0 mL/min. Peaks were detected at 254 nm and each peak was identified by LC/MS. The reproducibilities (RSD) of this proposed method, on the basis of peak-area ratios from six replicate injections, were 0.93-1.42% (intraday) and 1.05-1.63% (interday) at a detection limit of 0.12-0.78 microg/mL. Most of the markers used in this study can be classified, respectively, into three major categories, namely, isoflavones, O-glycosidic isoflavones, and C-glycosidic isoflavones. The structures of the compounds were determined from LC-MS fragmentation data and data from the literature.

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Toxicological evaluation of the isoflavone puerarin and its glycosides

Chung

Houng

et al. 2009

Eur Food Res Technol

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A Simultaneous Determination of Daidzin and Puerarin and Determination of Daidzein in Oriental Pharmaceutical Decoctions Containing Puerariae Radix by Ion-Pair High-Performance Liquid Chromatography

Cited by 8 publications

References 0 publications

Quantification of puerarin in plasma by on-line solid-phase extraction column switching liquid chromatography–tandem mass spectrometry and its applications to a pharmacokinetic study

Quantification of puerarin in plasma by on-line solid-phase extraction column switching liquid chromatography–tandem mass spectrometry and its applications to a pharmacokinetic study

Determination of 12 pueraria components by high‐performance liquid chromatography‐mass spectrometry

Toxicological evaluation of the isoflavone puerarin and its glycosides

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