A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

Chen, Shee‐Uan; Ho, Hong Nerng; Chao, Kuang Han; Huang, Senlin; Lee, Tzu Yao; Yang, Yu Shih

doi:10.1007/bf02766133

Cited by 5 publications

(3 citation statements)

References 20 publications

(19 reference statements)

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“…Similar results have previously been reported on two-needle biopsied embryos in the mouse (Santalo et al, 1995) and human . The in vitro viability data are also in line with the experience of Chen et al (1997), where a single-needle approach was tested in an in vitro mouse system. A small but significant reduction of developmental capacity was, however, observed when compared with nonmanipulated control embryos, indicating some damage caused by the in vitro manipulations.…”

Section: Discussionsupporting

confidence: 75%

Application of single-needle blastomere biopsy in human preimplantation genetic diagnosis

Inzunza

Iwarsson

Fridström³

et al. 1998

Prenat. Diagn.

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We have tested and subsequently successfully applied a single‐needle approach to obtain blastomere biopsies from human preimplantation embryos for preimplantation genetic diagnosis (PGD). The method was first evaluated in a mouse system and shown to be compatible with a high degree of in vitro and in vivo development of biopsied mouse embryos. Furthermore, we showed that biopsied mouse embryos after transfer to recipient mice underwent implantation, normal development and delivery. Litters were followed through puberty and adulthood and shown to be normal with regard to sexual function and also a panel of biochemical and morphological parameters including organ histology. Successful human preimplantation diagnosis, followed by pregnancies and birth of healthy babies, was established with two out of three couples carrying a risk to transmit chromosomal abnormalities leading to severe disease. This is the first report of the successful use of a single‐needle approach in human PGD. Considering its simplicity, we conclude that the single‐needle approach is an attractive alternative for biopsies in PGD. Copyright © 1998 John Wiley & Sons, Ltd.

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Section: Discussionsupporting

confidence: 75%

Application of single-needle blastomere biopsy in human preimplantation genetic diagnosis

Inzunza

Iwarsson

Fridström³

et al. 1998

Prenat. Diagn.

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“…This is an advantageous feature in the practice, as only a simple, inexpensive control unit is needed for the holder if the inverted microscope has a movable stage. Unlike the method of Chen et al (1997), the capillary does not need to be refilled with AT solution after each biopsy, thus the work can be done much faster.…”

Section: Characterisation Of Progenymentioning

confidence: 99%

A simplified biopsy method for precompacted mouse embryos: A technical report

Bodó

Laczkó

Horváth

2002

Acta Veterinaria Hungarica

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This article presents a new, simple and rapid embryo biopsy method. The blastomere for genetic analysis can be separated from a precompacted mouse embryo after a partial zona digestion with the use of a holding pipette. For the micromanipulation only two microcapillaries and micromanipulators are needed. The development of the biopsied embryos was studied during in vitro culture and in utero following embryo transfer. There was no significant difference between the treated and the control groups in the ratio of embryos that developed to the blastocyst stage, although the biopsied embryos were delayed in their development because they contained significantly fewer cells compared to the control ones at the same stage. Although there was no difference in the ratio of implantation, the development of the biopsied embryos in utero was also delayed 12-24 hours on the 9th day of pregnancy. No difference in development was visible from the 13th day of pregnancy. Statistically, no differences were found in the developmental ratio (number of developed fetuses/transferred embryos) of the control and treated embryos during gastrulation (9th day of pregnancy), at the beginning of organogenesis (13th day of pregnancy) and before birth (19th day of pregnancy). The embryo biopsy method presented here can be a new and useful tool for preimplantation genetic diagnosis.

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“…Zona dissection refers to making an opening in the ZP to allow the biopsy micropipette to go through and extract some sample material for genetic tests. The opening can be created by controlling a micropipette to penetrate the cell [145], releasing the acid Tyrode's solution around the target zone [146], or firing the laser once the ablation zone is at the spot [147]. In recent decades, laser zona dissection has gradually become the most popular option for zona dissection as it is more e cient, accurate, and safe [124].…”

Section: Image Processing For Selecting Optimal Dissection Positionmentioning

confidence: 99%

Vision-guided robotic micromanipulation for cell rotation control

Cui

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Ang Wei Tech, for his invaluable guidance and insightful suggestions throughout my Ph.D. study at Nanyang Technological University. He always encouraged me and provided generous support every time I got lost, not only in my research but also in daily life. His enthusiasm and optimism for research and work keep inspiring me to live a meaningful life, both now and in the future.Secondly, I would like to sincerely express my appreciation to my thesis advisory committee, Prof. Lum Guo Zhan, for his warm help in my exploration of several research projects and valuable sharing of study and life, and Prof. Hu Guoqiang for his kind advice on my research progress.Many thanks also go to my fellow teammates,

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A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

Cited by 5 publications

References 20 publications

Application of single-needle blastomere biopsy in human preimplantation genetic diagnosis

Application of single-needle blastomere biopsy in human preimplantation genetic diagnosis

A simplified biopsy method for precompacted mouse embryos: A technical report

Vision-guided robotic micromanipulation for cell rotation control

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