A Simplified Protocol for the Histological Preparation of Cortical Bone Samples for Light Microscopy: A Revision of Garcia-Donas et al. (2017)

Maggio, Ariane; Flavel, Ambika; Franklin, Daniel

doi:10.5744/fa.2020.1003

Cited by 4 publications

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“…The slides were prepared following Maggio et al (2020). The bone fragments were gently cleaned with a soft toothbrush to remove any adherent soil.…”

Section: Slide Preparationmentioning

confidence: 99%

Differentiating human from non‐human bone fragments through histomorphological assessment of remains from Camposanto cemetery, Italy

et al. 2022

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When bones are found within archaeological contexts it is important for site interpretation that they are identified by species, or at the very least as human or non‐human. However, key landmarks and characteristics typically used for such an assessment are often destroyed when bones are highly fragmented; thus methods including DNA or histology are amongst the few remaining options. Histological analysis of cortical tissue can be performed to discriminate human from non‐human origin. A set of 40 bone fragments recovered during excavation of the Camposanto cemetery on the island of Lazzaretto Nuovo (Venice, Italy), used to accommodate mass plague deaths from 1468 ce, were histologically determined to be of non‐human origin. Histomorphometric analysis of secondary osteons and Haversian canals, and non‐metric assessment of plexiform, osteonal and non‐plexiform fibrolamellar tissue, were applied to determine the most probable species origin. Discriminant function analysis established by Martiniaková and co‐workers in 2006, was applied, resulting in 36 samples classified as pig and four as cattle. This is consistent with the island’s use as a quarantine station during this period when imported cattle were housed to avoid the spread of disease. These results further enhance the understanding of the Lazzaretto Nuovo site.

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“…The slides were prepared following Maggio et al (2020). The bone fragments were gently cleaned with a soft toothbrush to remove any adherent soil.…”

Section: Slide Preparationmentioning

confidence: 99%

Differentiating human from non‐human bone fragments through histomorphological assessment of remains from Camposanto cemetery, Italy

et al. 2022

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The professional practice of forensic anthropology: Contemporary developments and cross‐disciplinary applications

Franklin

Marks

2021

WIREs Forensic Science

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The modern practice of forensic anthropology is focused on the quantification of skeletal variation based upon a qualitative prowess developed according to theory and method in the parent discipline of biological anthropology. Such morphological variances allow estimates of biological attributes, for example, age, sex, population affinity, stature, pathology of disease processes-among others, that collectively may resolve the identification of unknown forensic human remains.The latter is a basic human right under International Humanitarian Law (IHL), and International Human Rights Law (IHRL) afforded to descendants who go missing in armed conflicts and other violence-base situations. The specific traditional methods applied by the forensic anthropologist are well established in the published literature and routinely supported by global research that guides graduate programs designed to train the next generation of practitioners. Important in that cornerstone literature and graduate training regimen are aspects of our practice that are increasingly cross-disciplinary, especially those grounded in the molecular, chemical, and histological sciences. These upper-tier endeavors supplement and many times, validate, the traditional approaches as dictated by case context. This review focuses on evolving cross-disciplinary developments and novel research trends in the analysis of human remains that involve the specific expertise of the forensic anthropologist.

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Species: Human vs. Non-Human

Franklin

Marks²

2023

Encyclopedia of Forensic Sciences, Third Edition

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A Simplified Protocol for the Histological Preparation of Cortical Bone Samples for Light Microscopy: A Revision of Garcia-Donas et al. (2017)

Cited by 4 publications

References 0 publications

Differentiating human from non‐human bone fragments through histomorphological assessment of remains from Camposanto cemetery, Italy

Differentiating human from non‐human bone fragments through histomorphological assessment of remains from Camposanto cemetery, Italy

The professional practice of forensic anthropology: Contemporary developments and cross‐disciplinary applications

Species: Human vs. Non-Human

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