A simplified method of demonstrating Giemsa-Band pattern in human chromosomes

Chaudhuri, J. P.; Vogel, Walther; Voiculescu, I.; Wolf, U.

doi:10.1007/bf00273042

Cited by 22 publications

(4 citation statements)

References 4 publications

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“…Divalent cations, such as Ca 2 + and Mg 2 + play a significant role in maintaining the integrity of the 250 A chromatin fiber (STUBBLEFIELD 197 3 ). Chelation of these ions with phosphate, citrate or EDTA also results in G-bands of human chromosomes (RmLER 1971;CHAUDHURI et al 1971;SuMNER et al 1971;WARBURTON et al 1972). The characteristic banding patterns of human chromosomes produced by anti-adenosine antiserum correspond closely to the Q-or G-bands .…”

Section: Resultsmentioning

confidence: 99%

“…Subsequent modifications of techniques demonstrate the differential banding patterns throughout the entire length of individual chromosomes, enabling the identification of all human and mouse chromosomes. In some of these methods, the so-called SSC-techniques, salt solutions at higher temperature EvANS et al 1971;RIDLER 1971;CHAUDHURI et al 1971) are used, presumably to cause denaturation of native DNA. In others, chromosome preparations are exposed to either proteolytic enzymes (SEABRIGHT 1971(SEABRIGHT , 1972WANG andfEDOROFF 1971) urea (SHRAISHI andYosHIDA 1972), chelating agents, or solutions deficient in divalent cations CN+ and Mi+ in order to modify the chromosome structure prior to staining.…”

Section: Introductionmentioning

confidence: 99%

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Staining Method for the Banding Patterns of Human Mitotic Chromosomes

1974

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Staining Method for the Banding Patterns of Human Mitotic Chromosomes

1974

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“…QM staining [3], GBG staining [4] and trypsin-Giemsa staining (Manolov, personal communication) were used. For the exact identification of the breakpoints prometaphase chromosomes were studied using MTX incorporation [14], BrdU release and acridine orange staining, giving an R-banding pattern.…”

Section: Cytogenetic Studiesmentioning

confidence: 99%

Familial inversion translocation (8;13) with partial trisomy 13 in several family members

Pilgaard

Jørgensen

et al. 1983

Eur J Pediatr

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Abstract.A partial trisomy 13q was observed in siblings with hexadactylia, hypertelorism, haemangiomata and severe psychomotor retardation. It originated from a maternal inversion translocation 46,XX,inv(8)(q23q241),t(8;13)(q241;q32). The family showed a pedigree pattern typical for the segregation of a chromosomal translocation. In spite of this the diagnosis was delayed several years, because the bands involved from the two chromosomes were of great similarity. This stresses the importance of reinvestigating families with a clinical suspicion of a chromosomal syndrome, preferentially with prometaphase chromosomes. The identification of a chromosomal rearrangement is essential for genetic counselling and prenatal diagnosis. Key words:Chromosome inversion translocation -Partial trisomy 13 -Prometaphase chromosomes -Genetic counseling -Congenital malformations -Mental retardation second malformed child, was born and the chromosomes of the infant and the parents were examined with Q and R banding. When a partial trisomy 13 was found in the proband, the chromosomes of her elder brother (IV, l) were evaluated again on the stored microphotographs. He was found to have the same chromosomal aberration as the proband. The mother carried a complicated balanced (8;13) translocation, so did the maternal grandfather. In generation II, seven malformed children died in early infancy. In generation III, two malformed infants died and there was one abortion. Medical records were available for three individuals in generation II (II,3, II,7 and II,9) and for two individuals in generation III (III,1, III,4). The information on generations II and III was collected from hospital records, family records, midwife records and parish files. The data from the two affected children in generation IV, were recorded in detail.

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“…The chromosome analysis was carried out on cultured fibroblasts from a post-mortem skin biopsy, and included G-banding (Chaudhuri et al 1972 sen 1975) ( Fig. 2).…”

Section: Letters To the Editormentioning

confidence: 99%

Letters to the Editor

Pedersen¹

1976

Clinical Genetics

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A simplified method of demonstrating Giemsa-Band pattern in human chromosomes

Cited by 22 publications

References 4 publications

Staining Method for the Banding Patterns of Human Mitotic Chromosomes

Staining Method for the Banding Patterns of Human Mitotic Chromosomes

Familial inversion translocation (8;13) with partial trisomy 13 in several family members

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