A Simplified Method for Predicting the Effect of Temperature on the Separation Performance by Chromatography

Abstract

A simplified method for predicting the effect of temperature on the separation performance by chromatography was developed. As a model separation system, two polyphenol component separation by polymer-resin chromatography with ethanol-water mobile phase was chosen. HETP values as a function of mobile phase velocity for a wide range of temperatures 15-45℃ were well correlated by a single universal cur ve in the dimensionless HETP and the dimensionless velocity plots. A simplified equation that can predict the resolution was derived. The equation includes the temperature dependence of the distribution coefficients and the molecular diffusion coefficients. The relative separation performance (resolution) calculated by this equation showed that the existence of the maximum resolution depends on the temperature dependence of the distribution coefficients. Even when the resolution decreases with temperature, higher temperature operations may be advantageous as both the separation time and the separation volume decrease.

A Method for Determining the Optimum Temperature for a Polyphenol Separation Process by Reversed Phase Chromatography

“…We have proposed a method for predicting the separation conditions based on the iso-resolution curve [8,9].…”

“…In our previous paper [8], a simple method for predicting the effect of temperature on the performance of LC was presented for a model separation system, two polyphenol separations by RPC with polymer-resins. Since both the retention of solutes and the peak widths are influenced not only by temperature but also by the mobile phase composition (ethanol-water mixture), finding the optimum condition is complicated.…”

“…In this study, a more elaborate method for determining the optimum temperature was developed based on the iso-resolution curve concept [9,10]. The same two-component model separation system as in our previous studies [8,9,10] was used. The model samples were two polyphenols, catechin and epigallocatechin gallate (EGCG).…”

Temperature Effect on the Separation Performance of Chromatography

“…With the dimensionless (reduced) HETP, h and the dimensionless (reduced) velocity v, the HETP equation ( 3) can be rewritten as follows (Carta and Jungbauer, 2010;Yamamoto et al, 1987;1988;Nozaki et al, 2021). (13)…”