A simplified method for inorganic selenium and selenoaminoacids speciation based on HPLC–TR–HG–AFS

Sánchez-Rodas, Daniel; Mellano, Fernanda; Morales, E.; Giráldez, Inmaculada

doi:10.1016/j.talanta.2012.11.005

Cited by 35 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Se efectuaron un total de 13 aplicaciones de 50 µg de Se por planta. La metodología para la optimización de los parámetros desarrollados en el análisis de las especies de selenio utilizadas en este ensayo, mediante un equipo de absorción de fluorescencia atómica, ha sido descrita por Sánchez-Rodas et al (2013).…”

Section: Materials Y Métodosunclassified

Efectos Del Enriquecimiento Con Selenio en Spad, Calidad De La Fruta Y Parámetros De Crecimiento De Plantas De Fresa en Un Sistema De Cultivo Sin Suelo

et al. 2016

View full text Add to dashboard Cite

EfEctos dEl EnriquEcimiEnto con sElEniorEsumEn -El selenio es un elemento traza esencial no solo para animales, plantas y microorganismos, sino también para la salud de las personas. Por ello, parece que aumentar las cantidades de selenio en plantas puede tener implicaciones positivas. El objetivo de este trabajo fue determinar en un sistema de cultivo sin suelo con fibra de coco el efecto del selenito (Se IV) y selenato (Se VI) en el peso, calidad de las frutas de fresa, así como en variables de crecimiento y contenido de clorofilas de las hojas jóvenes y adultas de la planta de fresa de la variedad 'Splendor'. Los tratamientos fueron: T1 Se(IV)F (foliar), T2 Se(IV)S (sustrato), T3 Se(VI)F, T4 Se(VI)S y T5 control (sin aporte de Se). Se aplicaron semanalmente las mismas concentraciones de Se(IV) y Se(VI). Los resultados del análisis mostraron que el efecto de los tratamientos sobre firmeza, sólidos solubles totales y acidez titratable en frutas fue significativo. Los valores más altos de firmeza y sólidos solubles fueron mostrados por el tratamiento T4. Los mayores valores para el pH se producen en los tratamientos con T2 y T4. Los resultados indican que el Se tiene un efecto positivo sobre los valores de SPAD en hoja vieja. Las plantas con tratamiento de selenio presentaron un mayor número de hojas en relación a las plantas control. términos para indexación: Fragaria x ananassa Duch. Sólidos solubles totales. Cultivo sin suelo. Ácido titratable. ºBrix. EffEcts of sE-EnricHmEnt on sPAd, fruit quAlity And GroWtH PArAmEtErs of strAWBErry PlAnts in soillEssGroWinG systEm ABstrAct -Selenium (Se) is an essential trace element for animals, plants, microorganisms and human health. Thus, it appears that increasing amounts of Se in plants could have positive implications. The aim of our study was to evaluate the influence of foliar and substrate application of selenite (Se IV) and selenate (Se VI) on weight, fruit quality, growth parameters and content of chlorophyll from old and new leaves of strawberry plants (Fragaria x ananassa Duch.) cv. 'Splendor' in a soilless growing system with coir fiber as substrate. The treatments were: T1 Se(IV)F (foliar), T2 Se(IV)S (substrate), T3 Se(VI)F, T4 Se(VI)S and T5 control (Without application of Se). The same concentrations of Se(IV) and Se(VI) were applied weekly.The results of analysis showed that the effect of treatments on firmness, total soluble solids and titratable acidity in fruit were significant. The highest values of firmness and total soluble solids were showed by treatment T4 Se(VI)S. T2 and T4 treatments showed the highest pH values. The results indicate that Se has a positive effect on SPAD values on old leaf. The strawberry plants with selenium tretaments has higher number of leaves than control plants. index terms: Fragaria x ananassa Duch. Total Soluble Solid. Soilless. Titratable acidity. ºBrix.

show abstract

Section: Materials Y Métodosunclassified

Efectos Del Enriquecimiento Con Selenio en Spad, Calidad De La Fruta Y Parámetros De Crecimiento De Plantas De Fresa en Un Sistema De Cultivo Sin Suelo

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Separation techniques herein could be classified into chromatographic methods and non-chromatographic methods. Among them, gas chromatography (GC) [10,11], high performance liquid chromatography (HPLC) [12,13] and capillary electrophoresis (CE) [14,15] are the most efficient separation techniques. Comparatively, non-chromatographic schemes are simple, sensitive and low cost.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous speciation of inorganic arsenic, selenium and tellurium in environmental water samples by dispersive liquid liquid microextraction combined with electrothermal vaporization inductively coupled plasma mass spectrometry

Liu

Chen

et al. 2015

Talanta

View full text Add to dashboard Cite

“…Aqua regia (nitric acid/hydrochloric acid = 1/3 ( v / v )) was added to the precipitate suspensions, mixed well, and neutralized with sodium hydroxide. Selenium and tellurium in the samples were determined by HG-AFS (Millennium Excalibur, PSA, Orpington, UK) as described previously [ 96 ]. The analysis conditions were as follows: injection volume, 100 μL; acid carrier, 50% ( v / v ) HCl; and reductant, 0.7% ( w / v ) NaBH 4 in 0.1 M NaOH.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Novel Porin-Like Protein, ExtI, from Geobacter sulfurreducens and Its Implication in the Reduction of Selenite and Tellurite

Jahan

Tobe

Mihara

2018

IJMS

View full text Add to dashboard Cite

The extI gene in Geobacter sulfurreducens encodes a putative outer membrane channel porin, which resides within a cluster of extHIJKLMNOPQS genes. This cluster is highly conserved across the Geobacteraceae and includes multiple putative c-type cytochromes. In silico analyses of the ExtI sequence, together with Western blot analysis and proteinase protection assays, showed that it is an outer membrane protein. The expression level of ExtI did not respond to changes in osmolality and phosphate starvation. An extI-deficient mutant did not show any significant impact on fumarate or Fe(III) citrate reduction or sensitivity to β-lactam antibiotics, as compared with those of the wild-type strain. However, extI deficiency resulted in a decreased ability to reduce selenite and tellurite. Heme staining analysis revealed that extI deficiency affects certain heme-containing proteins in the outer and inner membranes, which may cause a decrease in the ability to reduce selenite and tellurite. Based on these observations, we discuss possible roles for ExtI in selenite and tellurite reduction in G. sulfurreducens.

show abstract

A simplified method for inorganic selenium and selenoaminoacids speciation based on HPLC–TR–HG–AFS

Cited by 35 publications

References 34 publications

Efectos Del Enriquecimiento Con Selenio en Spad, Calidad De La Fruta Y Parámetros De Crecimiento De Plantas De Fresa en Un Sistema De Cultivo Sin Suelo

Efectos Del Enriquecimiento Con Selenio en Spad, Calidad De La Fruta Y Parámetros De Crecimiento De Plantas De Fresa en Un Sistema De Cultivo Sin Suelo

Simultaneous speciation of inorganic arsenic, selenium and tellurium in environmental water samples by dispersive liquid liquid microextraction combined with electrothermal vaporization inductively coupled plasma mass spectrometry

Characterization of a Novel Porin-Like Protein, ExtI, from Geobacter sulfurreducens and Its Implication in the Reduction of Selenite and Tellurite

Contact Info

Product

Resources

About