A simplified method for cyanogen bromide activation of agarose for affinity chromatography

Identification of a new P1 residue mutation (444Arg→Ser) in a dysfunctional C1 inhibitor protein contained in a type II hereditary angioedema plasma

“…Anti-C1 inhibitor IgG was prepared from a rabbit anti-Cl inhibitor antiserum as described previously [18], and coupled to Sepharose CL-4B using the procedure of March et al [22].…”

In vivo treatment of Heymann's Nephritis using a cytotoxic protein‐toxin conjugate

“…|mmunonosuppressants like FK506 [20] and cyclosporin A [13], also have been used in the therapy of immunemediated diseases. However, their nonspecific and generalised action on the immune system, usually necessitates antibiotic therapy to ward of infections as occurring as a secondary phenomenon.…”

“…Sephacryl S-300 and Sepharose 4B were purchased from Pharmacia Biotech, Sweden. Lentil lectin was prepared according to the method of Hayman and Crumpton [12] and coupled to Sepharose 4B as described by March et al [13]. The sample was tested for purity by SDS-PAGE as described by Kerjaschki and Farquhar [7].…”

“…The IgG fraction was purified using Protein G-Sepharose. The purified IgG was coupled to Sepharose 4B according to the method of March et al [13].…”

Ca2+—calmodulin dependent myosin light‐chain phosphorylating activity in insulin‐secreting tissues

“…This was done to remove endogenous calmodulin which remained bound to the column. The eluate, which contained 90% of the initial protein was adjusted to 2 mM CaCl, and chromatographed on a 2 X 25 cm affinity column prepared by coupling 20 mg bovine brain calmodulin to 80 ml Sepharose 4B by the CNBr method [27].…”