A simplified chromatographic method for quantitative determination of coenzyme Q10 in dog plasma

Total Coenzyme Q10 Concentrations in Asian Men Following Multiple Oral 50-mg Doses Administered as Coenzyme Q10 Sustained Release Tablets or Regular Tablets.

“…The determination method was established by improving the extraction recoveries. [5][6][7] The uses of methanol to precipitate protein in plasma and n-hexane to extract the CoQ 10 and CoQ 9 improved the extraction efficiency. CoQ 10 and the internal standard CoQ 9 are unstable under the light, thus all the samples were wrapped with aluminium foil and treatments were carried out under the illumination of yellow light or in the dark.…”

Analysis of coenzyme Q10 in human plasma by column-switching liquid chromatography

“…Quantitative determination of CoQ 10 in the solventextracted plasma without any purification was found unsuitable for routine determinations according to some authors [11,18] owing to the large solvent front, the dirty injected sample and the short column lifetime. TLC [14] had been used as an effective tool to purify the extraction of plasma, but a poor recovery and accuracy were obtained.…”

“…Many analytical methods have been reported for quantitative determination of CoQ 10 in human plasma [5][6][7][8][9][10][11][12][13][14][15], such as spectrophotometric [5,6], voltammetric [7] and chemiluminescent [8]. Among these publications, the most popular tools used for assaying are HPLC [9][10][11][12][13][14][15], because of the complexity of biological fluid specimens, they usually required samples purified before injection. Thin layer chromatography (TLC) and solid phase extraction (SPE) pretreatment method have been described in several literatures [11][12][13][14], those manual purification methods are time and money consuming, unsuitable for routine determination, especially for clinical chemistry laboratories.…”

“…Among these publications, the most popular tools used for assaying are HPLC [9][10][11][12][13][14][15], because of the complexity of biological fluid specimens, they usually required samples purified before injection. Thin layer chromatography (TLC) and solid phase extraction (SPE) pretreatment method have been described in several literatures [11][12][13][14], those manual purification methods are time and money consuming, unsuitable for routine determination, especially for clinical chemistry laboratories. In this paper, a procedure of determining CoQ 10 with on-line sample purification is investigated based on column-switching HPLC, resulting in an automatic analysis method, in the meantime, the CoQ 10 levels in plasma of healthy subjects and the thyroid patients were determined.…”

Validated HPLC method for the quantitative determination of CoQ10 in dog plasma and its application to a pharmacokinetic study

“…Many analytical procedures have been reported to quantify CoQ 10 in biosamples: HPLC with ultraviolet detection (UV; Vadhanavikit et al, 1984;Grossi et al, 1992;Kommuru et al, 1998;Lu et al, 2003;Jiang et al, 2004;Karpinska et al, 2006;Li et al, 2006;Hatanaka et al, 2008;Balakrishnan et al, 2009;Paliakov et al, 2009), HPLC with electrochemical detection (ECD; Edlund, 1988;Okamoto et al, 1988;Yamashita and Yamamoto. 1997;Menke et al, 2000;Tang et al, 2001Tang et al, , 2004Mosca et Galinier et al, 2004;Miles et al, 2008), and LC coupled with mass spectrometry (MS) (Hansen et al, 2004;Li et al, 2008;Prosek et al, 2008).…”