A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth

Bakkar, Mohammed; Liu, Younan; Fang, Dongdong; Stegen, Camille; Su, Xiu; Ramamoorthi, Murali; Lin, Li-Chieh; Kawasaki, Tatsuya; Makhoul, Nicholas; Pham, Huan; Sumita, Yoshinori; Tran, Simon D.

doi:10.1007/978-1-4939-6756-8_15

Cited by 26 publications

(18 citation statements)

References 12 publications

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“…Dental pulp mesenchymal stem/stromal cells (DP-MSCs) are of particular interest because of their neurotropic character, which makes DP-MSCs and their exosomes particularly attractive as a new therapeutic tool for the alleviation of symptoms of neurodegenerative diseases and many other difficultly treatable maladies. Dental tissue-derived stem cells besides DP-MSCs include multiple types such as stem cells from exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP), periodontal ligament stem cells (PDLSCs), and dental follicle progenitor cells (DFPCs) [ 4 ]. All of them can be isolated from a single tooth and behave as mesenchymal stem/stromal cells.…”

Section: Introductionmentioning

confidence: 99%

Dental Mesenchymal Stem/Stromal Cells and Their Exosomes

Stanko

Altanerova²,

Jakubechova³

et al. 2018

Stem Cells International

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Stem cells derived from human dental pulp tissue (DP-MSC) differ from the other mesenchymal stem cells prepared from bone marrow or adipose tissue due to their embryonic origin from the neural crest and are of special interest because of their neurotropic character. Furthermore, the therapeutic potential of DP-MSCs is realized through paracrine action of extracellularly released components, for which exosomes play an important role. In this review, we intend to explore the properties of these cells with an emphasis on exosomes. The therapeutic applicability of these cells and exosomes in dental practice, neurodegenerative diseases, and many other difficultly treatable diseases, like myocardial infarction, focal cerebral ischemia, acute lung or brain injury, acute respiratory distress syndrome, acute inflammation, and several others is concisely covered. The use of cellular exosomes as an important diagnostic marker and indicator of targeted cancer therapies is also discussed, while the importance of stem cells from human exfoliated deciduous teeth as a source of evolutionally young cells for future regenerative therapies is stressed. We conclude that exosomes derived from these cells are potent therapeutic tools for regenerative medicine in the near future as clinical administration of DP-MSC-conditioned medium and/or exosomes is safer and more practical than stem cells.

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Section: Introductionmentioning

confidence: 99%

Dental Mesenchymal Stem/Stromal Cells and Their Exosomes

Stanko

Altanerova²,

Jakubechova³

et al. 2018

Stem Cells International

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“…Stromal cells used for this study were harvested from periodontal ligament of 31-year-old female, according to the protocol described (Bakkar et al 2017 ) (Institutional Review Board protocol nr 1072.6120.253.2017). PDLSC (periodontal ligament stromal cells) were seeded directly onto material foils or tissue culture plastic as a control (TCP, Nest) at a density of 2 × 10 4 /cm 2 in 1 mL of medium (84% Alpha-MEM Gibco, 15% FBS Gibco, 1% ZellShield Minerva Biolabs).…”

Section: Methodsmentioning

confidence: 99%

Design, characterization and in vitro evaluation of thin films enriched by tannic acid complexed by Fe(III) ions

et al. 2020

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Materials based on carbohydrate polymers may be used for biomedical application. However, materials based on natural polymers have weak physicochemical properties. Thereby, there is a challenge to improve their properties without initiation of toxicity. The alternative method compared to toxic chemical agents’ addition is the use of metal complexation method. In this study, chitosan/tannic acid mixtures modified by Fe(III) complexation are proposed and tested for potential applications as wound dressings. Thereby, surface properties, blood compatibility as well as platelet adhesion was tested. In addition, the periodontal ligament stromal cells compatibility studies were carried out. The results showed that the iron(III) addition to chitosan/tannic acid mixture improves properties due to a decrease in the surface free energy and exhibited a reduction in the hemolysis rate (below 5%). Moreover, cells cultured on the surface of films with Fe(III) showed higher metabolic activity. The current findings allow for the medical application of the proposed materials as wound dressings.

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“…Increasing viability and expansion range of DP-SCs requires use of mallet (alternatively chisel) and cutback of frictional heat in the first place when isolating dental pulp from a single tooth [17]. First investigation to obtain DPSCs through explant culture of dental pulp resulted in harvesting heterogeneous subpopulation of cells [18].…”

Section: Culture and Isolationmentioning

confidence: 99%

Human Dental Pulp Stem Cells: recent findings and current research

Janowicz

Mozdziak

Bryja

et al. 2019

Medical Journal of Cell Biology

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Prevalence of neurodegenerative diseases, most of which are life threatening and incurable, is an increasing clinical problem. To date, studies have demonstrated a superior proliferation rate of dental pulp stem cells (DPSCs) compared to other mesenchymal stem cells in vitro. DPSCs has recently been recognized as a novel treatment strategy for neurodegenerative disease, due to their advanced potential for neurogenic differentiation. The oral cavity has been described as a promising source of dental pulp stem cells. DPSCs are widely used in regenerative dentistry holding alternative capacity for osteogenic differentiation and therefore new promises for tissue and whole tooth regeneration. Dental stem cell banking offers a plentiful source of stem cells representing great potential for cell reprogramming and thus cell therapy. Recently, the association of pulp stem cells with three – dimensional scaffold templates allows for building up naturally derived implants. This review introduces to unique properties of DPSCs and biological factors influencing mineralization, proliferation and differentiation of pulp stem cells. Latest research studies are compared in terms of effectiveness and limitations of techniques for the isolation of pulp stem cells, including the enzymatic digestion and the explant culture methods. Moreover, a short overview of most recent findings and clinical application of DPSCs is proffered including progress of current research and limitations still to be addressed in the nearest future. Finally, the article presents new advances in the area of regenerative dentistry and regenerative medicine, including three dimensional printing and three dimensional analysis, emerged to deepen studies under procedures to replace the non patient specific artificial implants.Running title: DPSCs - review

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A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth

Cited by 26 publications

References 12 publications

Dental Mesenchymal Stem/Stromal Cells and Their Exosomes

Dental Mesenchymal Stem/Stromal Cells and Their Exosomes

Design, characterization and in vitro evaluation of thin films enriched by tannic acid complexed by Fe(III) ions

Human Dental Pulp Stem Cells: recent findings and current research

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