A Simplified and Efficient Method for Production of Manganese Ferrite Magnetic Nanoparticles and Their Application in DNA Isolation

Abstract

A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe2O4) magnetic nanoparticles (MNPs). In addition to the wide applicability of MnFe2O4 MNPs, this work also reports their application in DNA isolation for the first time. An ultrasonic-cavitation-assisted combustion method was applied in the synthesis of MnFe2O4 MNPs at different furnace temperatures (573 K, 623 K, 673 K, and 773 K) to optimize the particles’ properties. It was shown that MnFe2O4 nanoparticles synthesized at 573 K consist of a spinel phase only with adequate size and zeta potential distributions and superparamagnetic properties. It was also demonstrated that superparamagnetic manganese ferrite nanoparticles bind DNA in buffer with a high NaCl concentration (2.5 M), and the DNA desorbs from the MNPs by decreasing the NaCl concentration of the elution buffer. This resulted in a DNA yield comparable to that of commercial DNA extraction products. Both the DNA concentration measurements and electrophoresis confirmed that a high amount of isolated bacterial plasmid DNA (pDNA) with adequate purity can be extracted with MnFe2O4 (573 K) nanoparticles by applying the DNA extraction method proposed in this article.

Manganese Nanoparticles: Synthesis, Mechanisms of Influence on Plant Resistance to Stress, and Prospects for Application in Agricultural Chemistry

“…53 55 Biocompatible Mn NPs associated with zinc (Zn) and iron (Fe) molecules have been created to obtain various derivatives of Mn 0.5 Zn 0.5 ErxY x Fe 2 -2xO 4 NPs using an ultrasonic approach. 56 MnFe 2 O 4 NPs can be also synthesized by ultrasonic cavitation combustion in a furnace at a temperature of 573 K. 57 Both methods for the synthesis of Mn NPs (chemical and physical) described above require high energy, use of toxic chemicals for recovery and packaging, and are not easily scaled up. The most important factor is that these methods are compromised by the use of toxic chemicals in the synthesis process, as these chemicals remain on the surface of the NPs even after repeated washing.…”

“…Biocompatible Mn NPs associated with zinc (Zn) and iron (Fe) molecules have been created to obtain various derivatives of Mn 0.5 Zn 0.5 ErxY x Fe 2 -2xO 4 NPs using an ultrasonic approach . MnFe 2 O 4 NPs can be also synthesized by ultrasonic cavitation combustion in a furnace at a temperature of 573 K …”

Simplified Synthesis of the Amine-Functionalized Magnesium Ferrite Magnetic Nanoparticles and Their Application in DNA Purification Method

“…The pDNA isolation procedure was carried out based on the protocol developed in our previous research on the use of manganese ferrite magnetic nanoparticles in DNA isolation applications [ 53 ]. An E. coli bacterial cell suspension was used for the isolation of a low-copy-number plasmid DNA called pBAD24.…”

Fabrication and characterization of rubidium ferrites by the solution‐based combustion method using diverse organic fuels

“…[3][4][5][6] Moreover, metal ferrites have garnered considerable attention due to their utility in high-density storage devices, humidity sensors, DNA separation, magnetic resonance imaging (MRI), and hyperthermia. [7][8][9][10][11][12][13] In recent times, nanosized alkali metal ferrites have attracted increasing interest, as they exhibit distinct properties compared to their bulk counterparts with the same composition. [14][15][16][17] The synthesis of nanoparticles with controlled size, shape, and composition holds immense scientific and technological significance, promising groundbreaking advancements in various nanotechnology and medical fields.…”