A simple U-HPLC-MS/MS method for the determination of liensinine and isoliensinine in rat plasma

Peng, Lisheng; Jiang, Xiaoyan; Li, Zhongxin; Yi, Tiegang; Huang, Bin; Li, Huilin; Zeng, Zhen; Liu, Yu; Peng, Song-Ling; He, Jinsong; He, Li

doi:10.1016/j.jchromb.2015.03.027

Cited by 7 publications

(17 citation statements)

References 10 publications

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“…Peng et al developed a simple U-HPLC-MS/MS method for the determination of liensinine and isoliensinine in rat plasma, and its application to a pharmacokinetic study after intravenous administration of 5.0 mg/ kg liensinine and isoliensinine, the t 1/2 was 9.28 ± 3.25 h, CL was 3.25 ± 1.00 L/h kg for liensinine [6]. Hu et al developed a simple, sensitive, and robust analytical method based on UPLC-MS/MS for the determination of the three alkaloids in rat plasma using carbamazepine as IS.…”

Section: Discussionmentioning

confidence: 99%

“…To better understand the pharmacokinetics, an analytical method for the determination of liensinine in biological fluids is necessary. There have several literatures been reported for determination of liensinine in rat plasma and its pharmacokinetic study [3][4][5][6]. Therefore, it was necessary to develop a UPLC-MS/MS method for determination of liensinine in mouse blood and its application for the pharmacokinetics.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

Tong¹,

Zeng

et al. 2021

Acta Chromatographica

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Liensinine is a bisbenzyltetrahydroisoquinoline alkaloid extracted from lotus (Nelumbo nucifera GAERTNER., Nelumbonaceae), especially in its embryo loti “Lien Tze Hsin” (green embryo of mature seed). A rapid and simple UPLC-MS/MS method was developed to determine liensinine in mouse blood and its application to a pharmacokinetic study. The blood samples were preprocessed by protein precipitation using acetonitrile. Midazolam (internal standard, IS) and liensinine were gradient eluted by mobile phase of methanol and water (0.1% formic acid) in a Waters UPLC BEH C18 column. The multiple reaction monitoring of m/z 611.3 → 206.1 for liensinine and m/z 326.2 → 291.1 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 400 ng/mL (r > 0.995). The accuracy ranged from 92.2 to 108.2%, the precision of intra-day and inter-day was less than 14%, and the matrix effect was between 100.0% and 109.6%, the recovery was better than 71.0%. The developed UPLC-MS/MS method was successfully used for a pharmacokinetic study of liensinine in mice after oral (5 mg/kg) and intravenous administration (1 mg/kg), and the absolute availability of liensinine was 1.8%.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

Tong¹,

Zeng

et al. 2021

Acta Chromatographica

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“…In comparison to conventional HPLC, ultra-high performance liquid chromatography (UHPLC) begins to dominate as the LC method of choice ( Gika et al, 2014 ) due to the much improved chromatographic resolution. Recently, UHPLC is gradually used in the metabolomic analysis of the lotus seeds ( Lv et al, 2015 ; Peng et al, 2015 ), while HPLC was a main method used before ( Chen et al, 2007 , 2012a ). Several modern chromatographic methods have also been developed for the analysis of metabolites in lotus seed.…”

Section: Metabolomics Study On the Lotus Seedsmentioning

confidence: 99%

“…The resolving power of the MS instrument is crucial for the identification of unknown compounds, and the metabolomic study on plant-derived food has entered a rapid development period with the widespread applications of HRMS ( Oms-Oliu et al, 2013 ). However, the metabolomic analysis of lotus seed selected LRMS as the major detection methods in the past, while the use of HRMS is far from satisfactory to date due to the expensive price and low penetration rate of HRMS ( Lin et al, 2013 ; Zhou et al, 2013 ; Li et al, 2014 ; Peng et al, 2015 ). In addition, in combination with spectrum detector such as UV or DAD, LC–MS can provide additional on-line structural information for each individual peak in a chromatogram, which has contributed to the identification of metabolites in lotus seeds.…”

Section: Metabolomics Study On the Lotus Seedsmentioning

confidence: 99%

“…As an important part of suffix omics, metabolomics is powerful in assessing safety and quality of food, assistant breeding, metabolism and pharmacokinetics of lotus seeds derived products, and so forth ( Oms-Oliu et al, 2013 ; Johanningsmeier et al, 2016 ). The wide application of metabolomics in lotus seeds has gained tremendous momentum in recent years ( Itoh et al, 2011 ; Chen et al, 2012a ; Li et al, 2014 ; Zhao et al, 2014 ; Hu et al, 2015 ; Jung et al, 2015 ; Peng et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Current Advances in the Metabolomics Study on Lotus Seeds

2016

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Lotus (Nelumbo nucifera), which is distributed widely throughout Asia, Australia and North America, is an aquatic perennial that has been cultivated for over 2,000 years. It is very stimulating that almost all parts of lotus have been consumed as vegetable as well as food, especially the seeds. Except for the nutritive values of lotus, there has been increasing interest in its potential as functional food due to its rich secondary metabolites, such as flavonoids and alkaloids. Not only have these metabolites greatly contributed to the biological process of lotus seeds, but also have been reported to possess multiple health-promoting effects, including antioxidant, anti-amnesic, anti-inflammatory, and anti-tumor activities. Thus, comprehensive metabolomic profiling of these metabolites is of key importance to help understand their biological activities, and other chemical biology features. In this context, this review will provide an update on the current technological platforms, and workflow associated with metabolomic studies on lotus seeds, as well as insights into the application of metabolomics for the improvement of food safety and quality, assisting breeding, and promotion of the study of metabolism and pharmacokinetics of lotus seeds; meanwhile it will also help explore new perspectives and outline future challenges in this fast-growing research subject.

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Development of CO2-switchable deep eutectic solvent-based liquid-liquid microextraction approach: Application in urinary excretion and tissue distribution studies

Yang,

Wang

et al. 2024

Analytica Chimica Acta

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A simple U-HPLC-MS/MS method for the determination of liensinine and isoliensinine in rat plasma

Cited by 7 publications

References 10 publications

Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

Current Advances in the Metabolomics Study on Lotus Seeds

Development of CO2-switchable deep eutectic solvent-based liquid-liquid microextraction approach: Application in urinary excretion and tissue distribution studies

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