A simple technique for quantifying apoptosis in 96-well plates

Ribble, Deborah; Goldstein, Nathaniel B.; Norris, David A.; Shellman, Yiqun G.

doi:10.1186/1472-6750-5-12

Cited by 641 publications

(294 citation statements)

References 27 publications

(13 reference statements)

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“…Determination of apoptosis and necrosis by acridine orange/ethidium bromide double staining -fluorescence microscopy analyses Acridine orange/ethidium bromide (AO/EB) double staining was performed according to Ribble et al [37]. AO and EB are DNA-binding fluorescent dyes.…”

Section: Assessment Of Mitochondrial Membrane Potential (δψM)mentioning

confidence: 99%

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Łaźniewska

Miłowska

Katir

et al. 2013

Cellular and Molecular Biology Letters

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Dendrimers containing viologen (derivatives of 4,4'-bipyridyl) units in their structure have been demonstrated to exhibit antiviral activity against human immunodeficiency virus (HIV-1). It has also recently been revealed that novel dendrimers with both viologen units and phosphorus groups in their structure show different antimicrobial, cytotoxic and hemotoxic properties, and have the ability to influence the activity of cholinesterases and to inhibit α-synuclein fibrillation. Since the influence of viologen-phosphorus structures on basic cellular processes had not been investigated, we examined the impact of such macromolecules on the murine neuroblastoma cell line (N2a). We selected three water-soluble viologen-phosphorus (VPD) dendrimers, which differ in their core structure, number of viologen units and number and type of surface groups, and analyzed several aspects of the cellular response. These included cell viability, generation of reactive oxygen species (ROS), alterations in mitochondrial activity, morphological modifications, and the induction of apoptosis and necrosis. The MTT assay results suggest that all of the tested dendrimers are only slightly cytotoxic. Although some changes in ROS formation and mitochondrial function were detected, the three compounds did not induce apoptosis or necrosis. In light of these results, we can assume that the tested VPD are relatively safe for mouse neuroblastoma cells. Although more research on their safety is needed, VPD seem to be promising nanoparticles for further biomedical investigation.

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Section: Assessment Of Mitochondrial Membrane Potential (δψM)mentioning

confidence: 99%

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Łaźniewska

Miłowska

Katir

et al. 2013

Cellular and Molecular Biology Letters

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“…10 l of each sample was dotted on a glass slide, covered with a coverslip, and counted using ϫ180 magnification on a Nikon SM1500 fluorescent microscope per Ref. 19. A minimum of 200 cells were counted per point, and grouped into four categories based on nuclear morphology and stain: viable (green with intact nuclei), necrotic (orange with intact nuclei), early apoptotic with intact membrane (green with fragmented nuclei), and late apoptotic with damaged membrane (orange with fragmented nuclei).…”

Section: Apoptosis Assays and Caspase Inhibitor Treatmentmentioning

confidence: 99%

Yaf2 Inhibits Caspase 8-mediated Apoptosis and Regulates Cell Survival during Zebrafish Embryogenesis

Stanton

McReynolds

Evans

et al. 2006

Journal of Biological Chemistry

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Rybp (DEDAF) is a member of the Rybp/Yaf2 protein family and has been shown to encode pro-apoptotic functions and to be essential for mouse embryogenesis. The related Yaf2 protein has not been studied extensively at the cellular or organismal levels. Here we describe zebrafish yaf2 (zyaf2) and show that it is widely expressed during early embryogenesis, with subsequent enrichment of transcripts in the anterior head region. Depletion of zYaf2 during embryogenesis using specific morpholinos activates a wide-spread program of apoptosis and causes developmental arrest before the one somite stage. Partial depletion of Yaf2, achieved by injecting lower dosages of morpholino, circumvents the early arrest but leads to CNS degeneration associated with excessive apoptosis. These phenotypes can be rescued by co-injection of human YAF2 mRNA with the morpholinos or by treatment with a pan-caspase inhibitor or a caspase 8-specific inhibitor. Finally, the observed activation of caspase 8 in the morphants is in accord with the ability of Yaf2 to inhibit caspase 8-mediated apoptosis in cultured cells. Our findings implicate Yaf2 as a survival factor during early zebrafish development and organogenesis. This may suggest that Yaf2 and Rybp can encode opposing functions in the regulation of apoptosis.

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“…Cell viability staining assay of the pneumococcal biofilm by acridine orange and ethidium bromide: To assess the effects of potential compounds on bacterial cell viability, an acridine orange/ethidium bromide staining assay was done according to the standard method [37,38]. The acridine orange/ethidium bromide reagent was made by mixing of 100 µg/mL of ethidium bromide and 100 µg/mL of acridine orange in PBS.…”

Section: Antimicrobial Activity By Agar Well Diffusion Methodmentioning

confidence: 99%

Synthesis of Some New Chrysin Derivatives and Their Biological Assessment as Antibacterial, Antibiofilm and Antifungal Agent

Bhowmik¹,

Daş²,

Ghosh³

et al. 2018

Asian J. Chem.

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Some new derivatives of natural chrysin have been synthesized and evaluated for antibacterial, antibiofilm and antifungal activities. Three compounds, namely 2a, 2e, 2i showed excellent activities (MIC 14.0-18.3 µg/mL) against six bacterial and two fungal type strains while most of the other compounds showed comparatively low to moderate values of such activities. Significant antibiofilm and bactericidal activities (MBC ranges from 17-20 µg/mL) were also observed for 2a, 2e, 2i against pneumonia causing two bacterial strains. This study further indicated that the chrysin compounds with 7-O-alkyl type substitution were much more effective than corresponding aryl substituted congeners which signifies that 7-O position of chrysin is crucial for antimicrobial activities. Thus, this article would contribute to further design and development of potential antimicrobial agents via structural modulation of natural chrysin, which is a potential molecular skeleton with diverse biological activities.

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A simple technique for quantifying apoptosis in 96-well plates

Cited by 641 publications

References 27 publications

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

Yaf2 Inhibits Caspase 8-mediated Apoptosis and Regulates Cell Survival during Zebrafish Embryogenesis

Synthesis of Some New Chrysin Derivatives and Their Biological Assessment as Antibacterial, Antibiofilm and Antifungal Agent

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