A simple system for staining protein and nucleic acid electrophoresis gels

Raymer, Dorian M.; Smith, Douglas E.

doi:10.1002/elps.200600325

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…Current silver‐staining methods start with treatment of the gel with fixing solution to prevent diffusion of protein bands and remove interfering substances, followed by several washings to eliminate any trace of fixing solution, before treatment with the silver soaking solution . In the present protocol, nitric acid and ethanol are added to the silver impregnating solution, thus combining fixing and soaking into a single step.…”

Section: Procedures For Improved Silver‐staining Methods For Proteinmentioning

confidence: 99%

A rapid and simplified method for protein silver staining in polyacrylamide gels

Zhao

Sun

et al. 2012

Electrophoresis

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Silver staining is widely used to detect protein in polyacrylamide gels when high sensitivity is required. A simple and rapid protocol for silver staining of proteins following PAGE was developed in the present study. The number of steps was reduced compared to conventional protocol by combining fixing, rinsing, and soaking into a single impregnating step, thus achieving detection of proteins in 20 min. The present method is as sensitive as current protocols with the advantage of saving time and costs.

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Section: Procedures For Improved Silver‐staining Methods For Proteinmentioning

confidence: 99%

A rapid and simplified method for protein silver staining in polyacrylamide gels

Zhao

Sun

et al. 2012

Electrophoresis

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2007

Biotechnology Journal

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Research Spotlight: J. Sep. Sci. 10/2008

2008

J of Separation Science

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A 2-D finite-volume model is developed to simulate nonlinear IEF in complex microgeometries. This mathematical model is formulated based on the mass conservation and ionic dissociation relations of amphoteric macromolecules, charge conservation, and the electroneutrality condition. Based on the 2-D model, three different separation cases are studied: an IPG in a planar channel, an ampholytebased pH gradient in a planar channel, and an ampholyte-based pH gradient in a contraction-expansion channel. The numerical results reveal different focusing efficiencies and resolution in the narrow and wide sections of the contraction-expansion channel. To explain this, the expressions for separation resolution and peak concentrations of separands in the contraction-expansion channel are presented in terms of the channel shape factor. In a 2-D planar channel, a focused band remains straight all the time. However, in a contraction-expansion channel, initially straight bands take on a crescent profile as they pass through the trapezoidal sections joining the contraction and expansion sections.[1] Electrophoresis 2007, 28, 572 -586.The pH transients that occur during isoelectric trapping separations as a result of the removal of nonampholytic ionic components have been re-examined.Salts containing strong electrolyte anions and cations, both with equal and dissimilar mobilities, have been studied using anodic and cathodic buffering membranes whose pH values were both equidistant and nonequidistant from pH 7. The direction and magnitude of the pH transient (acidic or basic) was found to depend on both the mobilities of the anion and cation (l anion /l cation ) and the pH difference between pH 7 and the pH of the buffering membranes (|pH memb anodic -7|/|7 -pH memb cathodic |). By selecting appropriate anodic and cathodic buffering membranes to adjust the |pH memb anodic -7|/|7 -pH memb cathodic | value, pH transients caused by dissimilar anion and cation mobilities can be avoided.[2] Electrophoresis 2007, 28, 587 -594.To study the molecular mass distribution and number of species in narrow-range (2-pH-unit wide, in the nominal pI 2-4 or 3-5 interval) carrier ampholytes from four commercial sources, a 2-D technique was adopted, consisting of a preparative focusing step in a Rotofor instrument, followed by analysis of every other collected fraction (10 out of 20) by CE-MS. It was found that Ampholine pH 3.5-5 contains 105 different molecular mass (M r ) compounds, in the Mr interval 205-965 Da, for a total of 446 isoforms. Bio-Lyte pH 3-5 consists of 84 different M r species, in the Mr range 216-965 Da, for a total of 383 isoforms. Servalyt pH 2-4 is made of 227 different M r compounds, in the Mr interval 204-929 Da, for a total of 1201 isoforms. Pharmalyte pH 2.5-5 comprises 245 amphoteres, in the M r range 203-857 Da, for a total of 857 isoforms. Pharmalyte appears to be the best brand, with the vast majority of species focusing sharply at their pI position and almost no , poor' species, distributed along the entire pH gradient, d...

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A simple system for staining protein and nucleic acid electrophoresis gels

Cited by 5 publications

References 2 publications

A rapid and simplified method for protein silver staining in polyacrylamide gels

A rapid and simplified method for protein silver staining in polyacrylamide gels

News & views: Paper watch

Research Spotlight: J. Sep. Sci. 10/2008

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