A simple system for biofilm potential monitoring in drinking water

Delahaye, E.; Lévi, Yves; Leblon, Gérard; Montiel, A.

doi:10.1002/jobm.200510005

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…The biological stability of drinking water in distribution systems is a major concern of water supply companies (14), consumers, regulators, and public health authorities alike (1,7). Bacterial regrowth within distribution systems is dependent upon a complex interaction of chemical, physical, operational, and engineering parameters (52).…”

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confidence: 99%

In Situ Monitoring of the Nascent Pseudomonas fluorescens Biofilm Response to Variations in the Dissolved Organic Carbon Level in Low-Nutrient Water by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy

Delille

Quilès

Humbert

2007

Appl Environ Microbiol

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Drinking water quality management requires early warning tools which enable water supply companies to detect quickly and to forecast degradation of the microbial quality of drinking water during its transport throughout distribution systems. This study evaluated the feasibility of assessing, in real time, drinking water biostability by monitoring in situ the evolution of the attenuated total reflectance-Fourier transform infrared (ATR-FTIR) fingerprint of a nascent reference biofilm exposed to water being tested. For this purpose, the responses of nascent Pseudomonas fluorescens biofilms to variations in the dissolved organic carbon (DOC) level in tap water were monitored in situ and in real time by ATR-FTIR spectroscopy. Nascent P. fluorescens biofilms consisting of a monolayer of bacteria were formed on the germanium crystal of an ATR flowthrough cell by pumping bacterial suspensions in Luria-Bertani (LB) medium through the cell. Then they were exposed to a continuous flow of dechlorinated sterile tap water supplemented with appropriate amounts of sterile LB medium to obtain DOC concentrations ranging from 1.5 to 11.8 mg/liter. The time evolution of infrared bands related to proteins, polysaccharides, and nucleic acids clearly showed that changes in the DOC concentration resulted in changes in the nascent biofilm ATR-FTIR fingerprint within 2 h after exposure of the biofilm to the water being tested. The initial bacterial attachment, biofilm detachment, and regrowth kinetics determined from changes in the areas of bands associated with proteins and polysaccharides were directly dependent on the DOC level. Furthermore, they were consistent with bacterial adhesion or growth kinetic models and extracellular polymeric substance overproduction or starvation-dependent detachment mechanisms.The biological stability of drinking water in distribution systems is a major concern of water supply companies (14), consumers, regulators, and public health authorities alike (1, 7). Bacterial regrowth within distribution systems is dependent upon a complex interaction of chemical, physical, operational, and engineering parameters (52). In particular, temperature, pH, disinfectant residual, hydrodynamic conditions, residence time, pipe materials, and above all the availability and composition of biodegradable organic matter are key factors in microorganism regrowth processes (37). Drinking water biostability is commonly assessed by biomass-based or biodegradability methods (37,45,47). Unfortunately, because of a slow response (usually more than 24 h) and/or batch processing these methods do not enable in situ real-time monitoring of microbiological quality. Moreover, culture-based methods do not reflect the actual dynamics of the attached bacterial films on pipe surfaces, which play a major role in postdisinfection biological regrowth (27,33). Consequently, in addition to these methods, managing finished water quality requires early warning devices which enable workers to detect quickly, or even to forecast, degradation of drink...

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confidence: 99%

In Situ Monitoring of the Nascent Pseudomonas fluorescens Biofilm Response to Variations in the Dissolved Organic Carbon Level in Low-Nutrient Water by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy

Delille

Quilès

Humbert

2007

Appl Environ Microbiol

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“…It is to be noted that, these biofilm reactors were reported to be highly adapted to industrial field conditions, for the monitoring of biofilm formation potentials in drinking water networks [14].…”

Section: Experimental Systemmentioning

confidence: 99%

Impact of orthophosphate addition on biofilm development in drinking water distribution systems

Gouider

Bouzid

Sayadi

et al. 2009

Journal of Hazardous Materials

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“…Toutefois, il est difficile d'étudier les biofilms formés dans le réseau de distribution en raison des contraintes industrielles occasionnées par le remplacement de sections de canalisation. Pour ce faire, des dispositifs expérimentaux ont été développés afin d'étudier la structure et la dynamique des biofilms formés sous l'action d'eau potable (BOE-HANSEN et al, 2003;DELAHAyE et al, 2006;KJELLERUP et al, 2004;MATHIEU et al, 2009;PARIS et al, 2007;PEREIRA et al, 2002;TORvINEN et al, 2007). Le principe de ces systèmes pilotes repose sur la colonisation de supports de même nature que les matériaux utilisés dans la construction des ouvrages de stockage et de distribution de l'eau potable.…”

Section: L'étude Des Biofilms Dans Le Système De Distibution D'eau Pounclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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L’émergence de pathogènes dans l’eau destinée à la consommation humaine représente une préoccupation majeure en matière de santé publique pour les industriels et les pouvoirs publics concernés. Parmi ces pathogènes, certains sont d’origine fécale (Cryptosporidium, Campylobacter ou bien les rotavirus), alors que d’autres vivent dans l’environnent naturel (Legionella, Pseudomonas, Aeromonas ou bien les mycobactéries). Dans l’optique de mettre en place une analyse des risques liés à la présence de ces pathogènes, il est important d’accroître nos connaissances sur l’écologie de ces microorganismes et de développer des outils d’analyse afin de réaliser une meilleure surveillance sanitaire. Par conséquent, l’écologie microbienne du réseau de distribution d’eau potable doit être étudiée en détail, particulièrement vis-à-vis des propriétés physiologiques et la diversité des espèces microbiennes présentes, afin de mieux comprendre les interactions entre les espèces communément rencontrées et celles pathogènes.The emergence of pathogens in water intended for human consumption is a major concern in terms of the public health industry and the public authorities concerned. Among these pathogens, some are of faecal origin (Cryptosporidium, Campylobacter, or rotavirus), while others live in the natural environment (Legionella, Pseudomonas, Aeromonas or mycobacteria). In order to establish a risk analysis related to the presence of these pathogens, it is important to increase our knowledge on the ecology of these microorganisms and to develop analytical tools to achieve better health monitoring. Therefore, the microbial ecology of drinking water distribution networks must be studied in detail, especially with respect to the properties and physiological diversity of the microbial species present, in order to better understand the interactions between species commonly encountered and those that are pathogens

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A simple system for biofilm potential monitoring in drinking water

Cited by 6 publications

References 17 publications

In Situ Monitoring of the Nascent Pseudomonas fluorescens Biofilm Response to Variations in the Dissolved Organic Carbon Level in Low-Nutrient Water by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy

In Situ Monitoring of the Nascent Pseudomonas fluorescens Biofilm Response to Variations in the Dissolved Organic Carbon Level in Low-Nutrient Water by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy

Impact of orthophosphate addition on biofilm development in drinking water distribution systems

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

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