A Simple Surface-Enhanced Raman Spectroscopic Method for on-Site Screening of Tetracycline Residue in Whole Milk

Dhakal, Shobhakar; Chao, Kuanglin; Huang, Qing; Kim, Moon J.; Schmidt, Walter F.; Qin, Jianwei; Broadhurst, C. Leigh

doi:10.3390/s18020424

Cited by 56 publications

(35 citation statements)

References 54 publications

(53 reference statements)

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“…When antibiotics are used, dairymen should follow specific protocols to prevent the prevalence of antibiotic residues in human milk. Despite that, reports show that there is still presence of antibiotics in milk, above the recommended values 1,6 . Their relative stability to the pasteurization processes and the high quantities of milk required to dilute milk from treated quarters, can cause monetary losses and technological problems to the dairy industry.…”

Section: Introductionmentioning

confidence: 95%

Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

Marques

Veigas

Araújo

et al. 2019

Sci Rep

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Throughout the last decade, the expansion of food testing has been gradually moving towards ordinary high throughput screening methods performed on-site. The demand for point-of-care testing, able to distinguish molecular signatures with high accuracy, sensitivity and specificity has been significantly increasing. This new requirement relies on the on-site detection and monitorization of molecular signatures suitable for the surveillance of food production and processing. The widespread use of antibiotics has contributed to disease control of livestock but has also created problems for the dairy industry and consumers. Its therapeutic and subtherapeutic use has increased the risk of contamination in milk in enough concentrations to cause economic losses to the dairy industry and have a health impact in highly sensitive individuals. This study focuses on the development of a simple Surface-Enhanced Raman Spectroscopy (SERS) method for fast high throughput screening of tetracycline (TET) in milk. For this, we integrate a paper-based low-cost, fully recyclable and highly stable SERS platform, with a minimal sample preparation protocol. A two-microliter sample of milk solutions spiked with TET (from 0.01 to 1000 ppm) is dried on a silver nanoparticle coated cardboard substrate and measured via a Raman spectrophotometer. The SERS substrate showed to be extremely stable with a shelf life of several months. A global spectrum principal component analysis approach was used to test all the detected vibrational modes and their correlation with TET concentration. Peak intensity ratios (455 cm−1/1280 cm−1 and 874 cm−1/1397 cm−1) were found to be correlated with TET concentrations in milk, achieving a sensitivity as low as 0.1 ppm. Results indicate that this SERS method combined with portable Raman spectrometer is a potential tool that can be used on-site for the monitoring of TET residues and other antibiotics.

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Section: Introductionmentioning

confidence: 95%

Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

Marques

Veigas

Araújo

et al. 2019

Sci Rep

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“…For instance, pesticide residues or illegal additives should be screened before exposure to consumers. [11][12][13][14] Explosives and drugs on luggage and clothing should be detected quickly and efficiently to avoid large numbers of passengers being stranded. [15,16] In the field of archaeological or cultural heritage studies, research on cultural heritage should be stringently noninvasive and nondestructive.…”

Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy for on‐site analysis: A review of recent developments

Gong

Wang

et al. 2020

Luminescence

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On‐site identification and quantification of chemicals is critical for promoting food safety, human health, homeland security risk assessment, and disease diagnosis. Surface‐enhanced Raman spectroscopy (SERS) has been widely considered as a promising method for on‐site analysis due to the advantages of nondestructive, abundant molecular information, and outstanding sensitivity. However, SERS for on‐site application has been restricted not only by the cost, performance, and portability of portable Raman instruments, but also by the sampling ability and signal enhancing performance of the SERS substrates. In recent years, the performance of SERS for on‐site analysis has been improved through portable Raman instruments, SERS substrates, and other combined technologies. In this review, popular commercial portable Raman spectrometers and the related technologies for on‐site analysis are compared. In addition, different types of SERS substrates for on‐site application are summarized. SERS combined with other technologies, such as electrochemical and microfluidics are also presented. The future perspective of SERS for on‐site analysis is also discussed.

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“…Additionally, due to narrow well-resolved bands characteristic of inelastic light scattering, SERS can provide abundant vibrational information of the adsorbed molecules and be applied to multiplex detection. In fact, this technique has been explored for the TC detection in several types of samples [23,24,25,26,27,28,29,30,31]. For instance, Li et al have applied Au hollow spheres to detect TC and the limit of detection reached 0.1 μg/L [31].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, Li et al have applied Au hollow spheres to detect TC and the limit of detection reached 0.1 μg/L [31]. Dhakal et al have reported a cyclodextrin-modified Ag colloid for on-site quantification of TC residues in whole milk, achieving a detection limit of 0.01 ppm, which is below the maximum residue limit (MRL) set by governmental authorities [27].…”

Section: Introductionmentioning

confidence: 99%

Magnetite-Supported Gold Nanostars for the Uptake and SERS Detection of Tetracycline

et al. 2018

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Magnetite nanoparticles (MNPs) decorated with gold nanostars (AuNSs) have been prepared by using a seed growth method without the addition of surfactants or colloidal stabilizers. The hybrid nanomaterials were investigated as adsorbents for the uptake of tetracycline (TC) from aqueous solutions and subsequent detection using surface-enhanced Raman scattering (SERS). Several parameters were investigated in order to optimize the performance of these hybrid platforms on the uptake and SERS detection of TC, including variable pH values and the effect of contact time on the removal of TC. The spatial distribution of TC and AuNS on the hybrid composites was accomplished by coupling SERS analysis with Raman imaging studies, allowing also for the determination of the detection limit for TC when dissolved in ultrapure water (10 nM) and in more complex aqueous matrices (1 μM). Attempts were also made to investigate the adsorption modes of the TC molecules at the surface of the metal NPs by taking into account the enhancement of the Raman bands in these different matrices.

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A Simple Surface-Enhanced Raman Spectroscopic Method for on-Site Screening of Tetracycline Residue in Whole Milk

Cited by 56 publications

References 54 publications

Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

Surface‐enhanced Raman spectroscopy for on‐site analysis: A review of recent developments

Magnetite-Supported Gold Nanostars for the Uptake and SERS Detection of Tetracycline

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