A simple spectrophotometric streptavidin–biotin binding assay utilizing biotin-4-fluorescein

Waner, Mark J.; Mascotti, David P.

doi:10.1016/j.jbbm.2007.06.001

Cited by 23 publications

(20 citation statements)

References 14 publications

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“…The number of avidin molecules bonded on each EP was 9.44 × 10 3 . 34,35 To prepare AuNP-coated EPs, 2.64 mg of the avEPs were mixed with various amounts of biotin-bonded AuNPs (bAuNPs). The avidin-biotin reaction allowed for tight binding of the AuNPs on the surface of the avEPs; the dissociation constant of avidin-biotin binding is approximately 10 -15 .…”

Section: Preparation Of Aunp-bound Epsmentioning

confidence: 99%

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

et al. 2017

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Herein, we propose a concept for sensing based on density changes of microparticles (MPs) caused by a biochemical reaction. The MPs are levitated by a combined acoustic-gravitational force at a position determined by the density and compressibility. Importantly, the levitation is independent of the MPs sizes. When gold nanoparticles (AuNPs) are bound on the surface of polymer MPs through a reaction, the density of the MPs dramatically increases, and their levitation position in the acoustic-gravitational field is lowered. Because the shift of the levitation position is proportional to the number of AuNPs bound on one MP, we can determine the number of molecules involved in the reaction. The avidinbiotin reaction is used to demonstrate the effectiveness of this concept. The number of molecules involved in the reaction is very small because the reaction space is small for an MP; thus, the method has potential for highly sensitive detection.

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Section: Preparation Of Aunp-bound Epsmentioning

confidence: 99%

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

et al. 2017

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“…Indeed, the BIOEGOFET platform, also matches the performances of customary fluorimetric and spectrophotometric assays which need labeled reagents. In particular, the LOD lies at the lower edge of the range of assays based on fluorescent biotin analogues (LOD ∼30 nM),36, 37 that allow, however, for a much narrower dynamic range.…”

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confidence: 99%

Electrolyte‐Gated Organic Field‐Effect Transistor Sensors Based on Supported Biotinylated Phospholipid Bilayer

et al. 2013

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“…The affinity of mSA was measured by FPS using biotinylated fluorescein as ligand. The ligand contains a 15 atom linker between biotin and fluorescein to minimize interference from the fluorophore, which may occur with a ligand containing a shorter linker, such as biotin‐4‐fluorescein (Aslan et al, 2005; Waner and Mascotti, 2008). The measured affinity, K d = 2.8 nM, is the highest among all non‐tetrameric streptavidin (Fig.…”

Section: Resultsmentioning

confidence: 99%

Novel heat resistant methyl‐tri(phenylethynyl)silane resin: Synthesis, characterization and thermal properties

Zhou

Feng

et al. 2006

J of Applied Polymer Sci

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Methyl-tri(phenylethynyl)silane (MTPES) was successfully synthesized by the reaction of lithium phenylacetylide with methyltrichlorosilane. The structure was characterized by HRMS, FTIR, Si-NMR, and elementary analysis. Thermal cure process was monitored by DSC, DMA, and FTIR. MTPES was heated to free flowing liquid around 1308C and thermally polymerized at 327-3778C to form thermoset. Thermal and oxidative properties were evaluated by TGA analysis. Thermoset exhibits extremely high heat-resistance and TGA curve in nitrogen shows the temperature of 5% weight loss (Td 5 ) of 6958C and total weight loss at 8008C of 7.1%. TGA shows a high Td 5 of 5658C even in air, although the total weight loss at 8008C was 56.1% of the initial weight, much higher than that in nitrogen. The high heat resistance of MTPES was ascribed to crosslinking reaction concerning ethynyl groups. Aging studies performed at elevated temperatures in air on a thermoset showed that MTPES is oxidatively stable to 3008C.

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A simple spectrophotometric streptavidin–biotin binding assay utilizing biotin-4-fluorescein

Cited by 23 publications

References 14 publications

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

Acoustic Sensing Based on Density Shift of Microspheres by Surface Binding of Gold Nanoparticles

Electrolyte‐Gated Organic Field‐Effect Transistor Sensors Based on Supported Biotinylated Phospholipid Bilayer

Novel heat resistant methyl‐tri(phenylethynyl)silane resin: Synthesis, characterization and thermal properties

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