A simple specific radioenzymatic assay for the simultaneous measurement of picogram quantities of norepinephrine, epinephrine, and dopamine in plasma and tissues

Sole, Michael J.; Hussain, M.

doi:10.1016/0006-2944(77)90064-3

Cited by 172 publications

(36 citation statements)

References 11 publications

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“…The catecholamines were extracted according to the method of Sole and Hussain (31) and separated by thin-layer chromatography according to Peuler and Johnson (32). The minimum detectable level of each catecholamine was 10 pg.…”

Section: Catecholamine Assaymentioning

confidence: 99%

Regulation of Episodic Growth Hormone Secretion by the Central Epinephrine System

Terry

Crowley

Johnson³

1982

J. Clin. Invest.

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A B S T R A C T Catecholamines are postulated to regulate growth hormone (GH) secretion by their influence on the release of two hypothalamic substances, somatostatin, which inhibits GH release, and GH-releasing factor, as yet unidentified. Extensive pharmacologic studies in man and animals indicate a stimulatory effect of central norepinephrine and dopamine on GH, but the function of epiphephrine (EPI) is uncertain. Furthermore, many of the agents used to study the role of catecholamines in GH regulation are not selective in that they affect adrenergic as well as noradrenergic and/or dopaminergic neurotransmission. In the present investigation, central nervous system (CNS) EPI

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Section: Catecholamine Assaymentioning

confidence: 99%

Regulation of Episodic Growth Hormone Secretion by the Central Epinephrine System

Terry

Crowley

Johnson³

1982

J. Clin. Invest.

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“…13 Elevated cardiac enzymes have also been found in patients with stroke compared with a variety of control populations. In a recent study, 4 Norris et al observed that 44 percent of 230 patients admitted to a Stroke Intensive Care Unit had elevated total CK levels and 11 percent had the cardiac isoenzyme CK-MB present when the CK was fractionated.…”

mentioning

confidence: 98%

Plasma norepinephrine in stroke.

Mg¹,

Norris²,

Hachniski³

et al. 1981

Stroke

247

122

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SUMMARY Cardiac arrhythmias, myocardial necrosis and ECG abnormalities in stroke may result from abnormally high levels of sympathetic activity. To examine this possibility, plasma norepinephrine, epinephrine and dopamine were measured in 74 patients with cerebral infarction, 18 with transient ischemie attacks and 33 non-stroke controls.Mean norepinephrine, epinephrine and dopamine values (pg/ml) in cerebral infarction (433.2, 81.6, 57.6) were higher (p < 0.01) than in controls (281.1, 60.1, 40.5, respectively). Transient ischemie attacks produced values intermediate to these two groups (319.3, 80.9, 54.9). The elevated catecholamine concentrations in cerebral infarction could not be explained by differences in age, blood pressure, heart rate, stress, type or severity of stroke. The high plasma norepinephrine in the stroke group is consistent with an increase in peripheral sympathetic activity which could produce the cardiac abnormalities of cerebral infarction.

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“…To each sample, 10 Al of EGTA was added as anticoagulant. Within an hour ofblood sampling, the blood samples were centrifuged and the plasma removed and deproteinized with 2 N HC104 and stored at -70 C. Analysis was performed by a radioenzymatic assay (1,26 Response to exercise. As seen in Fig.…”

Section: Methodsmentioning

confidence: 99%

Regulation of glucose turnover during exercise in pancreatectomized, totally insulin-deficient dogs. Effects of beta-adrenergic blockade.

Björkman¹,

Miles²,

Wasserman³

et al. 1988

J. Clin. Invest.

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To examine whether glucose metabolic clearance increases and whether catecholamines influence glucose turnover during exercise in total insulin deficiency, 24-h fasted and insulin-deprived pancreatectomized dogs were studied before and during exercise (60 min; 100 m/min; 10% slope) with (a = 8) and without (a = 8) propranolol infusion (PI, 5 jsg/kg-min). Exercise with or without PI was accompanied by four and fivefold increments in norepinephrine and epinephrine respectively, while glucagon (extrapancreatic) fell slightly. Basal plasma glucose and FFA concentrations and rates of tracer-determined (3[3Hjglucose) hepatic glucose production (R.) and total glucose clearance (including urinary glucose loss) were 459±24 mg/dl, 1.7±0.5 mmol/liter, 7.8±0.9 mg/kg-min and 1.6±0.1 ml/kg-min, respectively. When corrected for urinary glucose excretion, basal glucose metabolic clearance rate (MCR) was 0.7±0.1 ml/kg-min and rose twofold (P < 0.0001) during exercise. Despite lower lactate (3.3±0.6 vs. 6.6±1.3 mmol/liter, P < 0.005) and FFA levels (1.1±0.2 vs. 2.2±0.2 mmol/liter, P < 0.0001) with PI, PI failed to influence MCR during exercise. R. rose by 3.7±1.7 mg/kg-min during exercise (P < 0.02) while with PI the increase was only 1.9±0.7 mg/kg-min (P < 0.002). Glucose levels remained unchanged during exercise alone but fell slightly with PI (P < 0.0001). Therefore, in total insulin deficiency, MCR increases marginally with exercise (13% of normal); the beta adrenergic effects of catecholamines that stimulate both FFA mobilization and muscle glycogenolysis do not regulate muscle glucose uptake. The exercise-induced rise in hepatic glucose production does not require an increase in glucagon levels, but is mediated partially by catecholamines. Present and previous data in normal and alloxandiabetic dogs, suggest that (a) in total insulin deficiency, control of hepatic glucose production during exercise is shifted from glucagon to catecholamines and that this may involve catecholamine-induced mobilization of peripheral substrates for gluconeogenesis and/or hepatic insensitivity to glucagon, and (b) insulin is not essential for a small exercise-induced increase in muscle glucose uptake, but normal insulin levels are

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A simple specific radioenzymatic assay for the simultaneous measurement of picogram quantities of norepinephrine, epinephrine, and dopamine in plasma and tissues

Cited by 172 publications

References 11 publications

Regulation of Episodic Growth Hormone Secretion by the Central Epinephrine System

Regulation of Episodic Growth Hormone Secretion by the Central Epinephrine System

Plasma norepinephrine in stroke.

Regulation of glucose turnover during exercise in pancreatectomized, totally insulin-deficient dogs. Effects of beta-adrenergic blockade.

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