A Simple Simultaneous Colorimetric Determination of Primary and Secondary Amines with Fluorescamine

Fluorometric determination of secondary amines based on their reaction with fluorescamine

“…4 nmol (14). The present fluorometric assay permits the determination of 0.5 nmol of secondary amines and is more sensitive than the previous methods (4,14).…”

“…Fluorescamine is known to react almost instantaneously with primary and secondary amines to give fluorescent pyrrolinones (FI) (1, 2) and nonfluorescent aminoenones (FII) (3,4), respectively (Scheme I). Fluorescamine itself is rapidly hydrolyzed to nonfluorescent products under reaction conditions (1).…”

“…DISCUSSION Two methods are available for the analysis of secondary amines by their reaction with fluorescamine. One is the colorimetric method consisting of measurement of the absorption of the aminoenone chromophores (FII) which have an absorption maximum at 310-330 nm (e 16000-18000) (4).…”

“…In the ultratrace analysis, lasers are very useful as the exciting sources for the fluorometric analysis, since the fluorescence intensity is proportional to the intensity of the exciting source. However, laser fluorometry is so sensitive that the detection limit is not always determined by the sensitivity of the apparatus but determined by the background signal from contaminant fluorescence (4). In order to reduce this solution blank, the temporal separation of the components has been achieved for the samples with a long fluorescence lifetime such as pyrene (30) and polycyclic aromatic hydrocarbons (31), since the emission lifetime of the background signal is relatively short.…”

Fluorometric determination of secondary amines with 2-methoxy-2,4-diphenyl-3(2H)-furanone

“…Fluorescamine (FLA) reacts with primary and secondary amines to produce fluorescent pyrrolinone (FI) and aminodienone-type chromophores (FII), respectively (1)(2)(3)(4). Recently, we have found that FII is easily converted by primary amines to FI (Scheme I), and developed a thin-layer chromatographic method for the fluorescence detection of secondary amines (5) and a fluorometric method for the determination of them (6) .…”